Characterization of cold-associated microRNAs in the freeze-tolerant gall fly Eurosta solidaginis using high-throughput sequencing

Lyons, Pierre J.; Govaere, Louise; Crapoulet, Nicolas; Storey, Kenneth B.; Morin, Pier Jr

doi:10.1016/j.cbd.2016.08.007

Cited by 36 publications

(18 citation statements)

References 41 publications

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“…MiR-1-3p was previously shown by deep sequencing to be abundant in the tick Hyalomma anatolicum anatolicum, the pinewood (Wei et al, 2009;Huang et al, 2010;Luo et al, 2015). Whereas earlier reports presented miR-1-3p up-regulation in cold-exposed models including the pinewood nematode (Huang et al, 2010) and the freeze-tolerant goldenrod gall fly (Lyons et al, 2016), miR-1-3p was not significantly altered in Colorado potato beetles subjected to low temperatures. The present approach also highlighted several miR-NAs, such as miR-277-3p and miR-276-5p, that were down-regulated in cold-exposed L. decemlineata (Table 3) and that had been reported to be regulated at low temperatures in other models of cold adaptation.…”

Section: Discussionmentioning

confidence: 79%

“…MiR‐1‐3p was previously shown by deep sequencing to be abundant in the tick Hyalomma anatolicum anatolicum , the pinewood nematode Bursaphelenchus xylophilus and the migratory locust Locusta migratoria (Wei et al ., ; Huang et al ., ; Luo et al ., ). Whereas earlier reports presented miR‐1‐3p up‐regulation in cold‐exposed models including the pinewood nematode (Huang et al ., ) and the freeze‐tolerant goldenrod gall fly (Lyons et al ., ), miR‐1‐3p was not significantly altered in Colorado potato beetles subjected to low temperatures.…”

Section: Discussionmentioning

confidence: 85%

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Amplification and quantification of cold‐associated microRNAs in the Colorado potato beetle (Leptinotarsa decemlineata) agricultural pest

Morin

Frigault

Lyons

et al. 2017

Insect Molecular Biology

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The Colorado potato beetle [Leptinotarsa decemlineata (Say)] is an important insect pest that can inflict considerable damage to potato plants. This insect can survive extended periods of cold exposure, and yet the molecular switches underlying this phenomenon have not been fully elucidated. A better characterization of this process would highlight novel vulnerabilities associated with L. decemlineata that could serve as targets for the management of this devastating pest. Using high-throughput sequencing, the current work reveals a cold-associated signature group of microRNAs (miRNAs) in control (15 °C) and -5 °C-exposed L. decemlineata. The results show 42 differentially expressed miRNAs following cold exposure including miR-9a-3p, miR-210-3p, miR-276-5p and miR-277-3p. Functional analysis of predicted targets associated with these cold-responsive miRNAs notably linked these changes with vital metabolic and cellular processes. Overall, this study highlights the miRNAs probably responsible for facilitating cold adaptation in L. decemlineata and implicates miRNAs as a key molecular target to consider in the development of novel pest management strategies against these insects.

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Section: Discussionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 85%

Amplification and quantification of cold‐associated microRNAs in the Colorado potato beetle (Leptinotarsa decemlineata) agricultural pest

Morin

Frigault

Lyons

et al. 2017

Insect Molecular Biology

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“…Anoxia-responsive miR-92b is a potent regulator of mTOR signaling that directly binds to its negative regulator (TSC1), therefore the lack of translational suppression of TSC1 during anoxia in the goldenrod gall fly could promote the inhibition of mTOR pro-proliferative functions (Lee et al, 2019). Follow-up high-throughput investigations focused on the ability of E. solidaginis to tolerate freezing, but seeing that freezing is a multi-factorial stressor that also requires adequate anoxia tolerance, it is interesting to note that the freezeinduced up-regulation of miR-137 and miR-31a is associated with the mitigation of reactive oxygen species and modulation of HIF-1α, respectively (Lyons et al, 2016). Future studies are required to determine if the same response would be elicited in an insect exposed to anoxia in the absence of freezing.…”

Section: Gall Fliesmentioning

confidence: 99%

The OxymiR response to oxygen limitation: a comparative microRNA perspective

Hadj‐Moussa

Storey

2020

Journal of Experimental Biology

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From squid at the bottom of the ocean to humans at the top of mountains, animals have adapted to diverse oxygen-limited environments. Surviving these challenging conditions requires global metabolic reorganization that is orchestrated, in part, by microRNAs that can rapidly and reversibly target all biological functions. Herein, we review the involvement of microRNAs in natural models of anoxia and hypoxia tolerance, with a focus on the involvement of oxygen-responsive microRNAs (OxymiRs) in coordinating the metabolic rate depression that allows animals to tolerate reduced oxygen levels. We begin by discussing animals that experience acute or chronic periods of oxygen deprivation at the ocean's oxygen minimum zone and go on to consider more elevated environments, up to mountain plateaus over 3500 m above sea level. We highlight the commonalities and differences between OxymiR responses of over 20 diverse animal species, including invertebrates and vertebrates. This is followed by a discussion of the OxymiR adaptations, and maladaptations, present in hypoxic high-altitude environments where animals, including humans, do not enter hypometabolic states in response to hypoxia. Comparing the OxymiR responses of evolutionarily disparate animals from diverse environments allows us to identify species-specific and convergent microRNA responses, such as miR-210 regulation. However, it also sheds light on the lack of a single unified response to oxygen limitation. Characterizing OxymiRs will help us to understand their protective roles and raises the question of whether they can be exploited to alleviate the pathogenesis of ischemic insults and boost recovery. This Review takes a comparative approach to addressing such possibilities.

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“…Small RNA libraries were prepared and sequenced following Ion Torrent protocols and as reported previously [ 40 ]. Samples were loaded on an Ion PI Chip v2 and sequenced with an Ion Proton Sequencer (Thermo Fisher Scientific, Waltham, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

Identification of Differentially Expressed miRNAs in Colorado Potato Beetles (Leptinotarsa decemlineata (Say)) Exposed to Imidacloprid

Morin

Lyons

Crapoulet

et al. 2017

IJMS

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The Colorado potato beetle (Leptinotarsa decemlineata (Say)) is a significant pest of potato plants that has been controlled for more than two decades by neonicotinoid imidacloprid. L. decemlineata can develop resistance to this agent even though the molecular mechanisms underlying this resistance are not well characterized. MicroRNAs (miRNAs) are short ribonucleic acids that have been linked to response to various insecticides in several insect models. Unfortunately, the information is lacking regarding differentially expressed miRNAs following imidacloprid treatment in L. decemlineata. In this study, next-generation sequencing and quantitative real-time polymerase chain reaction (qRT-PCR) were used to identify modulated miRNAs in imidacloprid-treated versus untreated L. decemlineata. This approach identified 33 differentially expressed miRNAs between the two experimental conditions. Of interest, miR-282 and miR-989, miRNAs previously shown to be modulated by imidacloprid in other insects, and miR-100, a miRNA associated with regulation of cytochrome P450 expression, were significantly modulated in imidacloprid-treated beetles. Overall, this work presents the first report of a miRNA signature associated with imidacloprid exposure in L. decemlineata using a high-throughput approach. It also reveals interesting miRNA candidates that potentially underly imidacloprid response in this insect pest.

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Characterization of cold-associated microRNAs in the freeze-tolerant gall fly Eurosta solidaginis using high-throughput sequencing

Cited by 36 publications

References 41 publications

Amplification and quantification of cold‐associated microRNAs in the Colorado potato beetle (Leptinotarsa decemlineata) agricultural pest

Amplification and quantification of cold‐associated microRNAs in the Colorado potato beetle (Leptinotarsa decemlineata) agricultural pest

The OxymiR response to oxygen limitation: a comparative microRNA perspective

Identification of Differentially Expressed miRNAs in Colorado Potato Beetles (Leptinotarsa decemlineata (Say)) Exposed to Imidacloprid

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