Characterization of collagen fibrils after equine suspensory ligament injury: An ultrastructural and biochemical approach

Exploring Effects of Protease Choice and Protease Combinations in Enzymatic Protein Hydrolysis of Poultry By-Products

“…The presence of protein bands with Mw above 100 kDa indicates the presence of undigested collagens. Earlier studies have shown that reduced α 1 - and α 2 -chains (monomeric form) from turkey collagen I migrated to approximately 110 and 120 kDa, while the β- and γ-bands (di- and trimeric forms, respectively) migrate to a Mw above 240 kDa [ 28 , 29 , 30 , 31 ]. Moreover, in an SDS-PAGE separating pepsin-solubilized turkey tendon collagen, Grønlien et al showed the presence of several forms of α collagen as well as the presence of β and γ forms of collagen molecules [ 32 ].…”

Surgical treatment of a second metacarpal bone exostosis with associated desmitis of the body of the suspensory ligament in a horse

“…The SLB is a heterogeneous structure, which consists of dense collagenous connective tissue surrounding muscle bundles and islands of adipose tissue (Shikh Alsook et al . ). The resulting variable echogenicity of this structure, together with the dorsoaxial location of the margins of the SL body relative to McII and the fourth metacarpal bone and inhibition of the passage of an ultrasound beam beyond the osseous interfaces of the splint bones makes accurate assessment of the extent of injury within the SL based on ultrasonography alone difficult.…”

“…Forelimb suspensory ligament densitometric analysis has indicated that the proportions of type III and type V collagen are higher in damaged tissues compared with normal tissues (Shikh Alsook et al . ). Concurrently, a decrease in type I collagen within damaged tissues has been recorded (Shikh Alsook et al .…”

“…Concurrently, a decrease in type I collagen within damaged tissues has been recorded (Shikh Alsook et al . ). These alterations could be responsible for the decrease in tissue quality and mechanical properties of a healing suspensory ligament, and explain why healing of the SL tends to be slow and with low quality repair tissue (Shikh Alsook et al .…”

“…These alterations could be responsible for the decrease in tissue quality and mechanical properties of a healing suspensory ligament, and explain why healing of the SL tends to be slow and with low quality repair tissue (Shikh Alsook et al . ). The MR images obtained 6 months post surgery showed complete bone healing with the absence of recurrence of excessive callus formation at the ostectomy site.…”