Rot fungi found in the exterior panelling of buildings must be able to withstand very varying conditions of temperature and moisture. In this study we have exposed wood samples with the brown-rot fungus Gloeophyllum scpiarium, often found in exterior panelling, to high and low temperatures, oxygen depletion, and drying. The activity of the fungi before and after the exposures was monitored by isothermal m icrocalori metry at 25 °C in order to assess the rate and level of recovery. The activity of the fungus was not greatly influenced by exposures (o 6, 37 and 45 °C. Higher temperatures (55 and 60°C) resulted in an initial period of low activity followed after 20-1 OOh by a recovery back to approximately the prc-exposure level. After Ih exposure to 65 °C no activity could be detected so the lethal temperature for this fungus is in the range of 60-65 °C. Low temperatures (-23 °C) resulted in reduced activity during 10-20h. The activity of the fungus decreased in closed vessels as a result of an increased concentration of carbon dioxide. When fresh air was admitted the activity wenl back to the level before the exposure. Wetting after drying returned the activity to the level it had before the drying.