Characterization of Copper-Resistant Agrobacterium Isolated from Legume Nodule in Mining Tailings

Ji, Yu; Fan, Lianmei; Yang, Shushen; Tang, Ming; Yang, Wenquan; Li, Huifen

doi:10.1007/s00128-008-9598-z

Cited by 7 publications

(2 citation statements)

References 10 publications

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“…Symbiotic Rhizobium species have been already isolated from nodules of another leguminous plant, Anthyllis vulneraria, growing in contaminated soils from a different mining district in Morocco [18]. The presence of Agrobacterium in nodules of plants in polluted soils was also previously described [33,34]. Herbaspirillum and Azospirillum are 2 root associated nitrogen fixing bacterium [9,35,36].…”

Section: Isolation and Identification Of Bacteriamentioning

confidence: 96%

Characterization of Root-Nodule Bacteria Isolated from Hedysarum spinosissimum L, Growing in Mining Sites of Northeastern Region of Morocco

Sbabou¹

2016

SOJMID

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expensive and harmful to soil integrity and microbial diversity [3,4]. Phytoremediation appears as a good alternative as plants can indeed bio-concentrate (phytoextraction) as well as bio-immobilize (phytostabilization) toxic metals through in situ rhizospheric processes [1,2]. However, mining sites in semi-arid areas are characterized by low vegetation cover due to the unfavorable effects of a combination of environmental factors, including metals toxicity, nutrient deficiency, poor soil structure and low water retention [5,6], thus, the development of a vegetative cover in these environments is a challenge. Recently, several studies have shown that the combination of plant-associated bacteria in phytoremediation may lead to promising results, in particular the group of plant growth promoting bacteria [7,8]. This approach is furthermore perceived as cost-effective, eco-friendly, and with good public acceptance [2]. The choice of bacteria is usually based on their potential to produce phytohormones such as IAA, gibberellins and cytokinins that directly promote roots and plant growth [9]. Other bacteria can synthesize organic chelators (siderophores) to acquire iron [10] that will play a positive role in plant nutrition; some have great potential for phosphate solubilization or possess ACC deaminase activity [11] while others are able to fix nitrogen [1] or provide protection against viral diseases [12]. In addition, microorganisms can be implicated directly in metal mobilization/ immobilization, by changing the metal bioavailability, solubility and toxicity by different mechanisms including alteration of soil pH, releasing of chelators (organic acids, biosurfactants, siderophores, polymeric substances or glycoprotein, etc), metal biosorption or by oxidation/ reduction reactions [4,13]. The use of plants for the remediation of contaminated soils by heavy metals obviously also depends on the plant species, and on its anatomical, physiological and molecular characteristics

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Section: Isolation and Identification Of Bacteriamentioning

confidence: 96%

Characterization of Root-Nodule Bacteria Isolated from Hedysarum spinosissimum L, Growing in Mining Sites of Northeastern Region of Morocco

Sbabou¹

2016

SOJMID

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“…Phylotypes belonging to Agrobacterium are Gram-negative heterotrophic bacteria and are usually found in soils, which cause tumors in plants (Nester, 2015). Some members of Agrobacterium are heavy metalresistant bacteria, such as Agrobacterium tumefaciens Frontiers in Environmental Chemistry frontiersin.org CCNWGS0286 isolated from a zinc-lead mine tailing (Hao et al, 2012) and the copper-resistant Agrobacterium tumefaciens strain type IAM 13129 isolated from root nodules of Lespedeza cuneata growing in a gold mining tailing region in the northwest of China (Yu et al, 2009). As such, Agrobacterium can survive the heavy metal concentrations applied in this experiment.…”

Section: Bacterial Communitymentioning

confidence: 99%

Changes in the bacterial and microeukaryotic communities in the bioreactor upon increasing heavy metal concentrations

Aceves-Suriano,

Montoya-Ciriaco,

Hernández-Guzmán

et al. 2023

Front. Environ. Chem.

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Heavy metals are necessary at low concentration for biological activity, but they are often toxic for microorganisms at high concentrations. Amplicon sequencing of 16S rRNA and 18S rRNA was used to investigate changes in the bacterial and microeukaryotic communities in an activated sludge bioreactor incrementally contaminated with nickel (Ni), copper (Cu), and zinc (Zn) with an IC50 value ranging from 0% to 100%, as previously determined, while an uncontaminated bioreactor served as a control. The chemical oxygen demand (COD) was on average 90% in the uncontaminated bioreactor but dropped to 49% when the heavy metal concentration was 100% IC50. The bacterial community in the uncontaminated bioreactor was dominated by Alphaproteobacteria (mostly Agrobacterium and Brevundimonas) when the heavy metal concentrations were low and Bacteroidetes (mostly Sphingobacterium) when the highest amounts of heavy metal concentrations were applied. The members of Ciliophora, Ascomycota, and Basidiomycota alternatively dominated in the uncontaminated bioreactor, while Ascomycota (mostly Fusarium) dominated in the contaminated bioreactor. The results revealed that increased concentrations of Ni, Cu, and Zn altered the bacterial and microeukaryotic communities and some putative metabolic functions.

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Identification of effective Pb resistant bacteria isolated from Lens culinaris growing in lead contaminated soils

Jebara

Abdelkerim

Fatnassi

et al. 2014

J. Basic Microbiol.

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Soil bacteria are a new phytoremediation system for the removal of heavy metals from soils. In this study, fifteen soil bacteria were isolated from root nodules of lentil growing in heavy metals contaminated soils, particularly by lead. Molecular characterization of the collection showed a large diversity, including Agrobacterium tumefaciens, Rahnella aquatilis, Pseudomonas, and Rhizobium sp. These soil bacteria had a wide range of tolerance to heavy metals. Among them, strains of A. tumefaciens and R. aquatilis tolerated up to 3.35 mM Pb; whereas Pseudomonas tolerated up to 3.24 mM Pb. The inoculation of lentil grown hydroponically with inoculums formed by these efficient and Pb resistant bacteria enhanced plant biomass. The treatment of this symbiosis by 1 mM Pb for 10 days or by 2 mM Pb for 3 days demonstrated that lentil had Pb accumulation capacity and can be considered a Pb accumulator plant, elsewhere, roots accumulated more Pb than shoots, and the inoculation decreased the Pb up take by the plants, suggesting that this symbiosis should be investigated for use in phytostabilization of Pb-contaminated soils. At the same time, a modulation in the antioxidant enzyme activity and a specific duration was required for the induction of the superoxide dismutase (SOD), peroxidase (POX), and ascorbate peroxidase (APX) response and to adapt to Pb stress. These results suggested that these enzymes may be involved in the main mechanism of antioxidative defense in lentil exposed to Pb oxidative stress.

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Characterization of Copper-Resistant Agrobacterium Isolated from Legume Nodule in Mining Tailings

Cited by 7 publications

References 10 publications

Characterization of Root-Nodule Bacteria Isolated from Hedysarum spinosissimum L, Growing in Mining Sites of Northeastern Region of Morocco

Characterization of Root-Nodule Bacteria Isolated from Hedysarum spinosissimum L, Growing in Mining Sites of Northeastern Region of Morocco

Changes in the bacterial and microeukaryotic communities in the bioreactor upon increasing heavy metal concentrations

Identification of effective Pb resistant bacteria isolated from Lens culinaris growing in lead contaminated soils

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