Characterization of Crystalline Calcium Pantothenate by Physicochemical Assay Methods

Frank, Michael J.; Trager, P.G.; Johnson, Jenell; Rubin, Saul H.

doi:10.1002/jps.2600620121

Cited by 4 publications

(3 citation statements)

References 12 publications

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“…A further confirmation of the identity of the product was the conversion of the ,8-alanine to pantothenate by pantothenate synthetase (Dpantoate:,B-alanine ligase [AMP forming]; EC 6.3.2.1) (16,19,24). The conversion to pantothenate required D-pantoate, ATP, and Mg2e and was confirmed by two-dimensional thinlayer chromatography (10).…”

Section: Resultsmentioning

confidence: 72%

Beta-alanine synthesis in Escherichia coli

Cronan¹

1980

J Bacteriol

117

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The enzyme, aspartate 1-decarboxylase (L-aspartate 1-carboxy-lyase; EC 4.1.1.15), that catalyzes the reaction aspartate-fl-alanine + C02 was found in extracts of Escherichia coli. panD mutants of E. coli are defective in 8-alanine biosynthesis and lack aspartate 1-decarboxylase. Therefore, the enzyme functions in the biosynthesis of the ,8-alanine moiety of pantothenate. The genetic lesion in these mutants is closely linked to the other pantothenate (pan) loci of E. coli K-12. D-Pantothenate, the condensation product of

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Section: Resultsmentioning

confidence: 72%

Beta-alanine synthesis in Escherichia coli

Cronan¹

1980

J Bacteriol

117

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“…It is stable at neutral pH but easily destroyed by acid, alkali and heat. − Calcium pantothenate (CP), a colorless and crystalline substance, is the main commercial form of pantothenic acid, because it is more stable and exists in solid state at room temperature. , CP has been extensively used as a constituent of multiple vitamin formulations and animal food supplements. Mostly of the marketed form of CP is amorphous, exhibiting high hygroscopicity and unfavorable chemical stability. − According to related references and supplier information, , the amorphous products need to be carefully handled in manufacturing, storage and transportation due to the high hygroscopicity, and it can suffer marked loss of activity by combination with other acidic vitamins such as ascorbic acid, thiamine, and pyridoxine hydrochloride. Therefore, discovering a more stable CP form is crucial for the application of CP.…”

Section: Introductionmentioning

confidence: 99%

“…Mostly of the marketed form of CP is amorphous, exhibiting high hygroscopicity and unfavorable chemical stability. 14−16 According to related references and supplier information, 17,18 the amorphous products need to be carefully handled in manufacturing, storage and transportation due to the high hygroscopicity, and it can suffer marked loss of activity by combination with other acidic vitamins such as ascorbic acid, thiamine, and pyridoxine hydrochloride. Therefore, discovering a more stable CP form is crucial for the application of CP.…”

Section: ■ Introductionmentioning

confidence: 99%

Improving Stability of Vitamin B5 Through Double Salt Formation

Xuan

Zhang

et al. 2021

Crystal Growth & Design

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Calcium pantothenate (CP) is a chemically unstable vitamin and sensitive to moisture, heat, acid, and alkali. The poor physicochemical properties have greatly limited its biological performance and applications. In this work, four new double salts of CP with pantoic acid calcium salt (PA), calcium isobutyrate (IBA), calcium β-hydroxy-β-methyl butyrate (HMB), and calcium lactate (LA) were synthesized aiming to reduce the moisture sensitivity and to enhance the stability. The three double salts (CP-PA, CP-IBA, CP-HMB) were fully characterized by single crystal X-ray diffraction, powder X-ray diffraction, differential scanning calorimetry, thermogravimetric analysis, and dynamic vapor sorption. In addition, their hygroscopicity and chemical stability under different conditions were also studied. The results show that all four double salts present improved hygroscopicity, physical stability, and chemical stability. Notably, compared to CP pure forms, CP-PA and CP-LA present significantly lower moisture absorption and obviously improved stability. This work provides a new method to improve the hygroscopicity, physical stability, and chemical stability of organic salts.

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