Characterization of CTX-M enzymes, quinolone resistance determinants, and antimicrobial residues from hospital sewage, wastewater treatment plant, and river water

Conte, Danieli; Palmeiro, Jussara Kasuko; Nogueira, Keite da Silva; Lima, Thiago Marenda Rosa de; Cardoso, Marco André; Pontarolo, Roberto; Pontes, Flávia Lada Degaut; Dalla-Costa, Líbera Maria

doi:10.1016/j.ecoenv.2016.10.031

Cited by 107 publications

(68 citation statements)

References 86 publications

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“…These sources of water are frequently polluted, in particular near cities [61]. Several studies provide much evidence that waste water effluent and surface water are important sources of the dissemination of ESBLs in the natural environment and in particular in Africa [71][72][73]. Third, we captured some bats by hanging nets on mango branches 2 m behind human dwellings and 1 km away from the hospital.…”

Section: Discussionmentioning

confidence: 99%

Characterization of ESBL-Producing Enterobacteria from Fruit Bats in an Unprotected Area of Makokou, Gabon

et al. 2020

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In Gabon, terrestrial mammals of protected areas have been identified as a possible source of antibiotic-resistant bacteria. Some studies on antibiotic resistance in bats have already been carried out. The main goal of our study was to detect extended-spectrum beta-lactamases (ESBLs) that are produced by enterobacteria from bats in the Makokou region in Gabon. Sixty-eight fecal samples were obtained from 68 bats caught in the forests located 1 km from the little town of Makokou. After culture and isolation, 66 Gram-negative bacterial colonies were obtained. The double-disk diffusion test confirmed the presence of ESBLs in six (20.69%) Escherichia coli isolates, four (13.79%) Klebsiella pneumoniae isolates, and one (3.45%) Enterobacter cloacae isolate. The analysis based on the nucleotide sequences of the ESBL resistance genes showed that all cefotaximase-Munichs (CTX-Ms) were CTX-M-15 and that all sulfhydryl variables (SHVs) were SHV-11: 41.67% CTX-M-15-producing E. coli, 16.67% CTX-M-15+SHV-11-producing E. coli, 8.33% CTX-M-15-producing K. pneumoniae, 25% CTX-M-15+SHV-11-producing K. pneumoniae, and 8.33% CTX-M-15-produced E. cloacae. This study shows for the first time the presence of multiresistant ESBL-producing enterobacteria in fruit bats in Makokou.

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Section: Discussionmentioning

confidence: 99%

Characterization of ESBL-Producing Enterobacteria from Fruit Bats in an Unprotected Area of Makokou, Gabon

et al. 2020

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“…The WWTP considered in this study is located in Tunis City, where municipal wastewater is treated, and therefore the occurrence of CTX-resistant isolates is highly predictive. Other works have also reported a high prevalence of CTX-resistant enterobacterial populations in WWTPs [Conte et al, 2017]. Beta-lactam-resistant enterobacteria from animal origins (livestock, domestic animals, wild animals) as well as in certain food products of animal origin (meat, eggs, and derivatives, etc.)…”

Section: Discussionmentioning

confidence: 99%

Multidrug Resistance and the Predominance of blaCTX-M in Extended Spectrum Beta-Lactamase-Producing Enterobacteriaceae of Animal and Water Origin

et al. 2018

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The aim of this work was the genetic characterization of cefotaxime-resistant enterobacteria from animals (53 samples), the surface water of rivers (17 samples), and wastewater treatment plants (43 samples) in Tunisia. A total of 48 (42.4%) cefotaxime-resistant isolates were recovered. An extended spectrum beta-lactamase (ESBL) phenotype with a positive double-disk synergy test (DDST) was exhibited by 34 (70.8%) and 14 (29.1%) isolates from water and animal origins, respectively. Isolates from water were identified as: Escherichia coli (n = 17), Hafnia spp. (n = 13), Citrobacter spp. (n = 1), Enterobacter cloacae (n = 1), Klebsiella pneumoniae (n = 1), and K. oxytoca (n = 1). Animal isolates were identified as: E. coli (n = 11), E. cloacae (n = 1), Hafnia spp. (n = 1), and K. pneumoniae (n = 1). PCR investigation of bla_CTX-M, bla_TEM, and bla_SHV genes showed that amongst the 48 isolates with a positive DDST, 41 (87.5%) carried the bla_CTX-M gene, 1 isolate harbored the bla_SHV gene, and 1 isolate coharbored bla_CTX-M with bla_SHV genes. The class 1 and 2 integrons were detected in 27 (56.2%) and 1 (2%) isolates, respectively. Our study showed a significant occurrence of ESBL-producing enterobacteria in animals and aquatic environments with a predominance of bla_CTX-M genes.

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“…Recent studies have reported the detection of elevated levels of clinically important AMR bacteria and ARGs in hospital effluent wastewater and environmental water sources that receive untreated hospital waste [39,46,47,50,58]. Clinically important AMR bacteria, including MDR (e.g., carbapenemase-producing Enterobacteriaceae) and ESBL-producing bacteria (e.g., ESBL-producing K. pneumoniae) [39,50,58] and vancomycin and ampicillin-resistant Enterococcus spp., have been identified in hospital wastewater-associated samples [46,47].…”

Section: Hospital Wastewater and The Dissemination Of Clinically Relementioning

confidence: 99%

“…Similarly, high levels of AMR were identified in industrial [66,77,92] and agricultural wastewater and samples recovered from pharmaceutical treatment plants [49,66]. High levels of clinically relevant ARBs and/or ARGs were identified in influent and effluent samples from hospital wastewater [36,39,40,42,50,54,58,76,80,82,83,90,94,97].…”

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confidence: 96%

The Contribution of Wastewater to the Transmission of Antimicrobial Resistance in the Environment: Implications of Mass Gathering Settings

et al. 2020

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Antimicrobial resistance (AMR) is the major issue posing a serious global health threat. Low- and middle-income countries are likely to be the most affected, both in terms of impact on public health and economic burden. Recent studies highlighted the role of resistance networks on the transmission of AMR organisms, with this network being driven by complex interactions between clinical (e.g., human health, animal husbandry and veterinary medicine) and other components, including environmental factors (e.g., persistence of AMR in wastewater). Many studies have highlighted the role of wastewater as a significant environmental reservoir of AMR as it represents an ideal environment for AMR bacteria (ARB) and antimicrobial resistant genes (ARGs) to persist. Although the treatment process can help in removing or reducing the ARB load, it has limited impact on ARGs. ARGs are not degradable; therefore, they can be spread among microbial communities in the environment through horizontal gene transfer, which is the main resistance mechanism in most Gram-negative bacteria. Here we analysed the recent literature to highlight the contribution of wastewater to the emergence, persistence and transmission of AMR under different settings, particularly those associated with mass gathering events (e.g., Hajj and Kumbh Mela).

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Characterization of CTX-M enzymes, quinolone resistance determinants, and antimicrobial residues from hospital sewage, wastewater treatment plant, and river water

Cited by 107 publications

References 86 publications

Characterization of ESBL-Producing Enterobacteria from Fruit Bats in an Unprotected Area of Makokou, Gabon

Characterization of ESBL-Producing Enterobacteria from Fruit Bats in an Unprotected Area of Makokou, Gabon

Multidrug Resistance and the Predominance of <b><i>bla</i></b><sub>CTX-M</sub> in<sub></sub> Extended Spectrum Beta-Lactamase-Producing Enterobacteriaceae of Animal and Water Origin

The Contribution of Wastewater to the Transmission of Antimicrobial Resistance in the Environment: Implications of Mass Gathering Settings

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