Characterization of Cu/Zn-SOD enzyme activities and gene expression in soybean under low nitrogen stress

Wang, Xiaobo; Zhang, Haowei; Gao, Yali; Zhang, Wenming

doi:10.1002/jsfa.7387

Cited by 19 publications

(15 citation statements)

References 26 publications

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“…In soybean, ten SOD isoenzymes were identified in different tissues (Wang et al, 2016). For example, one MnSOD isoenzyme was mainly detected in stems and seeds.…”

Section: Identification and Phylogenetic Analysis Of Sod Genes In Soymentioning

confidence: 99%

“…The photosynthetic rate, sugar production, N metabolism and amino acid production were also significantly inhibited under alkaline stress (at high pH) (Hu et al, 2015). Further, alkaline stress has affected 434 million ha of land and limited the crop productivity worldwide (Chen et al, 2018), although single form of soybean SOD gene has been identified under various stresses (Gopavajhula et al, 2013;Wang et al, 2016). It is necessary to explore the roles of soybean SOD family under environmental stresses, especially under alkaline stress.…”

Section: Introductionmentioning

confidence: 99%

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Genome-wide identification and characterization of the soybean SOD family during alkaline stress

Duanmu

Qiao

et al. 2020

PeerJ

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Background Superoxide dismutase (SOD) proteins, as one kind of the antioxidant enzymes, play critical roles in plant response to various environment stresses. Even though its functions in the oxidative stress were very well characterized, the roles of SOD family genes in regulating alkaline stress response are not fully reported. Methods We identified the potential family members by using Hidden Markov model and soybean genome database. The neighbor-joining phylogenetic tree and exon-intron structures were generated by using software MEGA 5.0 and GSDS online server, respectively. Furthermore, the conserved motifs were analyzed by MEME online server. The syntenic analysis was conducted using Circos-0.69. Additionally, the expression levels of soybean SOD genes under alkaline stress were identified by qRT-PCR. Results In this study, we identified 13 potential SOD genes in soybean genome. Phylogenetic analysis suggested that SOD genes could be classified into three subfamilies, including MnSODs (GmMSD1–2), FeSODs (GmFSD1–5) and Cu/ZnSODs (GmCSD1–6). We further investigated the gene structure, chromosomal locations and gene-duplication, conserved domains and promoter cis-elements of the soybean SOD genes. We also explored the expression profiles of soybean SOD genes in different tissues and alkaline, salt and cold stresses, based on the transcriptome data. In addition, we detected their expression patterns in roots and leaves by qRT-PCR under alkaline stress, and found that different SOD subfamily genes may play different roles in response to alkaline stress. These results also confirmed the hypothesis that the great evolutionary divergence may contribute to the potential functional diversity in soybean SOD genes. Taken together, we established a foundation for further functional characterization of soybean SOD genes in response to alkaline stress in the future.

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“…In soybean, ten SOD isoenzymes were identified in different tissues (Wang et al, 2016). For example, one MnSOD isoenzyme was mainly detected in stems and seeds.…”

Section: Identification and Phylogenetic Analysis Of Sod Genes In Soymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genome-wide identification and characterization of the soybean SOD family during alkaline stress

Duanmu

Qiao

et al. 2020

PeerJ

View full text Add to dashboard Cite

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“…Meanwhile, we observed that down-regulated gma-miR398c displayed the function of a major gene that negatively regulated the expression of multiple peroxisome-related genes (e.g., GmCSD1a/b, GmCSD2a, and GmCCS) ( Figure 2B). The enzymes encoded by these target genes represent the first protective barrier that efficiently eliminates free radicals and prevents plants from accumulating ROS to toxic [38] reported that GmCZ-SOD1 (Glyma05g04170) may be important in the adaptive evolution of nitrogen limitation in soybean, whereas it was identified as GmCCS (Glyma.05g055000) encoding a copper chaperone for superoxide dismutase in phylogenetic analysis ( Figure S5A). We determined that GmCCS is expressed predominantly in chloroplasts (Figure 4), similar to homologous genes in Arabidopsis [39].…”

Section: Discussionmentioning

confidence: 99%

Integration of sRNA, degradome, transcriptome analysis and functional investigation reveals gma-miR398c negatively regulates drought tolerance via GmCSDs and GmCCS in transgenic Arabidopsis and soybean

Zhou

Liu

et al. 2020

Preprint

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Background: Drought conditions adversely affect soybean growth, resulting in severe yield losses worldwide. Increasing experimental evidence indicates miRNAs are important post-transcriptional regulators of gene expression. However, the drought-responsive molecular mechanism underlying miRNA-mRNA interactions remains largely uncharacterized in soybean. Meanwhile, the miRNAregulated drought response pathways based on multi-omics approaches remain elusive. Results: We combined sRNA, transcriptome and degradome sequencing to elucidate the complex regulatory mechanism mediating soybean drought resistance. One-thousand transcripts from 384 target genes of 365 miRNAs, which were enriched in the peroxisome, were validated by degradome-seq. An integrated analysis showed 42 miRNA-target pairs exhibited inversely related expression profiles.Among these pairs, a strong induction of gma-miR398c as a hub gene negatively regulates multiple peroxisome-related genes ( GmCSD 1a/b, GmCSD 2a/b/c and GmCCS ). Meanwhile, we detected that alternative splicing of GmCSD1a/b might affect soybean drought tolerance by bypassing gma-miR398c regulation. Overexpressing gma-miR398c in Arabidopsis thaliana L. resulted in decreased percentage germination, increased leaf water loss, and reduced survival under water deficiency, which displayed sensitivity to drought during seed germination and seedling growth. Furthermore, overexpressing gma-miR398c in soybean decreased GmCSD 1a/b, GmCSD 2a/b/c and GmCCS expression, which weakened the ability to scavenge O 2 .− , resulting in increased relative electrolyte leakage and stomatal opening compared with knockout miR398c and wild-type soybean under drought conditions. Conclusion: The study indicates that gma-miR398c negatively regulates soybean drought tolerance, and provides novel insights useful for breeding programs to improve drought resistance by CRISPR technology. BackgroundSoybean (Glycine max L.) is the most important legume crop that provides essential vegetable proteins and oil sources for livestock and humans, while its production is severely constrained by global climatic changes, particularly drought stress [1]. However, the current lack of knowledge 4 regarding the regulatory network underlying soybean responses to drought conditions has restricted an effective management of soybeans productivity in the world. Therefore, characterizing the complex molecular mechanism of drought resistance is a prerequisite for improving soybean yieldIn plants, small RNAs play a prominent regulatory role influencing the adaptability to drought stress, especially microRNAs (miRNAs) via degradation or translational inhibition of their target genes [3]. In the past decade, some differentially expressed miRNAs (DEMs) under drought-simulated stress have been filtered by small RNA sequencing (sRNA-seq), mainly in soybean roots [4, 5]. However, only few miRNAs in soybean, e.g. gma-miR394 and gma-miR396, by overexpressing in model plants have been verified to perform an important function in response to drought str...

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“…Many countries in the world, such as China, are facing the problems of excessive nitrogen application and low nitrogen utilization e ciency in winter wheat production [1]. Excessive application of nitrogen fertilizer is the main reason for the low nitrogen utilization e ciency in wheat [2]. At the same time, it causes environmental pollution and becomes a threat of the sustainable development of agriculture.…”

Section: Introductionmentioning

confidence: 99%

“…The up-regulated expression of alternative oxidase (AOX) consumes excess sugars, and then the induced AOX balanced the carbon and nitrogen under nitrogen de ciency [12][13][14]. GmCZ-SOD1 gene was highly induced in soybean root under nitrogen de ciency [2]. The transcriptome showed 1799 maize differentially expressed genes (DEGs) involving multiple pathways under nitrogen de ciency [11].…”

Section: Introductionmentioning

confidence: 99%

Transcription strategies related to photosynthesis and nitrogen metabolism of wheat in response to nitrogen deficiency

Liu

Yin

et al. 2020

Preprint

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Background: It is a great challenge to reduce nitrogen application without yield reduction in agricultural production. Screening response gene related to important pathways will be helpful to understand the physiology, metabolism, and morphology response of wheat (Triticum aestivum) under nitrogen deficiency condition. Results: We conducted a hydroponic experiment with two nitrogen levels, which were complete nutrient solution (N1) and nutrient solution without nitrogen (N0). The results showed that the wheat phenotype were greatly changed under nitrogen deficiency, e.g., the decreased crop height, leaf area, root volume, photosynthetic rate, crop weight, and the increased root length, root surface area and root/shoot ratio. After a comprehensive analysis of the phenotype, transcriptome, GO pathways and KEGG pathways of wheat under nitrogen deficiency, we found that, the up-regulated Exp (24 genes) and Nrt (9 genes) families members were the positive response related to the increase of nitrogen absorption; the down-regulated Pet (3 genes), Psb (8 genes), Nar (3 genes) and Nir (1 genes) were related to the limitation of photosynthesis and nitrogen metabolism. Conclusions: This study provides 48 reference genes for improving photosynthesis and nitrogen metabolism of wheat under nitrogen deficiency, which can provide molecular markers for gene editing breeding.

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Characterization of Cu/Zn-SOD enzyme activities and gene expression in soybean under low nitrogen stress

Abstract: GmCZ-SOD is important for adaptability of soybean to nitrogen limitation and these results provide useful information to unravel its biological role in low nitrogen resistance in plants. © 2015 Society of Chemical Industry.

Cited by 19 publications

References 26 publications

Genome-wide identification and characterization of the soybean SOD family during alkaline stress

Genome-wide identification and characterization of the soybean SOD family during alkaline stress

Integration of sRNA, degradome, transcriptome analysis and functional investigation reveals gma-miR398c negatively regulates drought tolerance via GmCSDs and GmCCS in transgenic Arabidopsis and soybean

Transcription strategies related to photosynthesis and nitrogen metabolism of wheat in response to nitrogen deficiency

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