Characterization of cucumber mosaic virus I. Molecular heterogeneity mapping of RNA 3 in eight CMV strains

Owen, J. B.; Palukaitis, Peter

doi:10.1016/0042-6822(88)90520-x

Cited by 105 publications

(73 citation statements)

References 21 publications

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“…And, although Fny and Pf both belong to CMV subgroup I, potato cultivars A6, Allegany and Andover were only susceptible to Fny-CMV, and not Pf-CMV, while the reverse was true for genotype 2x(V-3)30. This difference in systemic invasion between two strains from the same CMV subgroup has been observed previously in tobacco (Owen and Palukaitis 1988). In addition, Jones and Latham (1996) observed that different CMV strains of the same subgroup could produce different responses in lupin.…”

Section: Discussionmentioning

confidence: 55%

Potato Resistance to Cucumber Mosaic Virus is Temperature Sensitive and Virus-Strain Specific

Celebi-Toprak

Slack

Russo³

2003

Breed. Sci.

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Cucumber mosaic virus (CMV) is an important plant pathogen worldwide, which infects and causes yield losses to many solanaceous crops but rarely to potatoes. In the study reported here, we have tested the susceptibility of various potato genotypes to three different CMV strains, Pf-CMV and Fny-CMV, which belong to subgroup I, and A9-CMV, a member of subgroup II. Eight potato genotypes were found that could be systemically infected by at least one of the three CMV strains. Furthermore, although most potato cultivars were resistant to systemic infection at 24°C, all became infected systemically when inoculated plants were grown at 30°C. These results suggested that the natural resistance that most potato crops express to CMV might be overcome under high-temperature growing conditions following infection, and that CMV resistance in potato showed virus strain specificity.

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Section: Discussionmentioning

confidence: 55%

Potato Resistance to Cucumber Mosaic Virus is Temperature Sensitive and Virus-Strain Specific

Celebi-Toprak

Slack

Russo³

2003

Breed. Sci.

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“…The phylogenetic analysis of these isolates based on complete MP sequence illustrated two major groups: I and II. The I-group was further divided into two subgroups: IA and IB (Owen and Palukaitis, 1988;Liu et al, 2009). The phylogenetic analysis revealed that the isolates under study were more closely related to the IB-subgroup along with other isolates reported from India and Japan (Srivastava et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

“…Many strains of CMV described from different parts of the world have been put into two subgroups, I and II, based on nucleic acid hybridization (Owen and Palukaitis, 1988), gene sequences (Owen et al, 1990;Szilassy et al, 1999), restriction fragment length polymorphism (RFLP) (Rizos et al, 1992), and serology (Hu et al, 1995). The diverse strains of CMV are divided into two main groups, I and II, according to their genomic sequences.…”

Section: Introductionmentioning

confidence: 99%

Partial characterization and development of sensitive and reliablediagnostic for the detection of cucumber mosaic virus

Khan¹

2015

Turk J Agric For

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A survey on Cucumber mosaic virus (CMV) was conducted on 21 banana orchards in three bananas producing states: Karnataka, Maharashtra, and Uttar Pradesh. Sampling was done during winter and autumn and all samples were initially examined for the presence of CMV using DAS-ELISA. Out of 300 tested samples, the presence of CMV was observed in 13. The lowest rate of infection was determined in Uttar Pradesh followed by Maharashtra, while high infections were observed in samples from Karnataka. Three CMV infected samples represented in each state were amplified through RT-PCR using the movement protein (MP) gene specific primers and cloned in the pGEMT essay vector. The RT-PCR products of amplified samples were sequenced. Gene sequences of CMV-KAR, CMV-MR, and CMV-UP isolates under study coding for the movement protein revealed 99.76%-99.88% and 99.28%-99.64% sequence identity at the nucleotide and amino-acid level. These isolates were compared with 29 CMV isolates reported from various plants around the world, which formed three distinct subclades: -IA, -IB, and -II. These isolates were most closely related to subgroup-I isolates, sharing up to 95.81% and 96.84% sequence identity at nucleotide and amino acid, respectively. However, these isolates shared 81.79%-81.90% and 82.80%-83.15% nucleotide and amino acid identity with subgroup II isolates. The isolates under study clustered with the CMV subgroup-IB as confirmed by phylogenetic analysis. Further, we standardized the minimum limit of RNA transcript for the sensitivity of RT-PCR with respect to the coat protein (CP) gene and movement protein (MP) gene. The results of RT-PCR showed that the CP gene was more sensitive than the MP gene for the detection of CMV as it amplified the gene product up to 0.02 ng RNA. The CP gene based RT-PCR assay may be a more sensitive, reliable, and convenient molecular tool for detection of the CMV pathogen, and can be used in quarantine, eradication, and tissue culture certification programs.

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“…Other cucumovirus (1-TAV, LS-CMV, Fny-CMV), stocks in our laboratory, have been described (Moriones et al, 1991 ;Owen & Palukaitis, 1988).…”

Section: Methodsmentioning

confidence: 99%

Differential interactions among isolates of peanut stunt cucumovirus and its satellite RNA.

Militao

Moreno

Rodríguez‐Cerezo

et al. 1998

Journal of General Virology

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The interactions of seven isolates of peanut stunt cucumovirus (PSV) originating from North America, Europe and Africa, and two variants of PSV satellite RNA (sat RNA) were analysed. Electrophoretic and immunoblot analyses of the coat protein (CP) and Northern blot hybridization analyses of the viral RNAs showed that isolates PSV F352, 1339 and 1507 belonged to subgroup I, and isolates PSV W, Su and B to subgroup II. The seventh isolate, robinia mosaic virus (RoMV) clustered with subgroup I isolates by CP analysis, but was related to both subgroups by RNA hybridization analysis. The ability to support the accumulation of two newly described sat RNA variants, P4 and P6 sat RNAs, was not

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Characterization of cucumber mosaic virus I. Molecular heterogeneity mapping of RNA 3 in eight CMV strains

Cited by 105 publications

References 21 publications

Potato Resistance to Cucumber Mosaic Virus is Temperature Sensitive and Virus-Strain Specific

Potato Resistance to Cucumber Mosaic Virus is Temperature Sensitive and Virus-Strain Specific

Partial characterization and development of sensitive and reliablediagnostic for the detection of cucumber mosaic virus

Differential interactions among isolates of peanut stunt cucumovirus and its satellite RNA.

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