Characterization of curdlan produced by <i>Agrobacterium</i> sp. <scp>IFO</scp> 13140 cells immobilized in a loofa sponge matrix, and application of this biopolymer in the development of functional yogurt

Martínez, Camila Ortiz; Ruiz, Suelen Pereira; Fenelon, Vanderson Carvalho; Morais, Gutierrez Rodriguês de; Baesso, Mauro Luciano; Matioli, Graciette

doi:10.1002/jsfa.7357

Cited by 20 publications

(20 citation statements)

References 39 publications

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“…It creates sterilizable, freezable and flavorless food gels, does not show toxicity and is approved by the Food and Drug Administration [ 10 ]. Curdlan can be used in novel, calorie-reduced products, because it is able to mimic the texture of foods containing fat [ 6 , 11 ]. Being a natural, biodegradable and biocompatible polymer, it is used in the engineering of biomaterials field for the production of 3D scaffolds or membranes for regenerative medicine applications [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of Gelation Temperature on the Molecular Structure and Physicochemical Properties of the Curdlan Matrix: Spectroscopic and Microscopic Analyses

Gieroba

Sroka-Bartnicka

Kazimierczak

et al. 2020

IJMS

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In order to determine the effect of different gelation temperatures (80 °C and 90 °C) on the structural arrangements in 1,3-β-d-glucan (curdlan) matrices, spectroscopic and microscopic approaches were chosen. Attenuated total reflection Fourier transform infrared spectroscopy (ATR FT-IR) and Raman spectroscopy are well-established techniques that enable the identification of functional groups in organic molecules based on their vibration modes. X-ray photoelectron spectroscopy (XPS) is a quantitative analytical method utilized in the surface study, which provided information about the elemental and chemical composition with high surface sensitivity. Contact angle goniometer was applied to evaluate surface wettability and surface free energy of the matrices. In turn, the surface topography characterization was obtained with the use of atomic force microscopy (AFM) and scanning electron microscopy (SEM). Described techniques may facilitate the optimization, modification, and design of manufacturing processes (such as the temperature of gelation in the case of the studied 1,3-β-d-glucan) of the organic polysaccharide matrices so as to obtain biomaterials with desired characteristics and wide range of biomedical applications, e.g., entrapment of drugs or production of biomaterials for tissue regeneration. This study shows that the 1,3-β-d-glucan polymer sample gelled at 80 °C has a distinctly different structure than the matrix gelled at 90 °C.

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Section: Introductionmentioning

confidence: 99%

Effect of Gelation Temperature on the Molecular Structure and Physicochemical Properties of the Curdlan Matrix: Spectroscopic and Microscopic Analyses

Gieroba

Sroka-Bartnicka

Kazimierczak

et al. 2020

IJMS

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“…Curdlan clumps as a strong gel totally in the thermal suspension and never return to aqueous state again 3 . Structural analysis of curdlan is given as β-(1→3) linkage with a homopolymer of Dglucan 4 . The degree of polymerization (DP) of unbranched curdlan was roughly measured as 450 5 .…”

Section: Article Can Be Accessed Online Onmentioning

confidence: 99%

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2018

IJPSR

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Biopolymers are gaining more importance in our dayto-day life, due to the hazardous effect of synthetic plastics in our environment. Curdlan is one of the biopolymers, which has a broad field of applications in the food, pharmaceutical and medical industries. It is a polymer of glucose, produced by the family members of Rhizobiaceae. Curdlan [(1→3)-β-D-glucan] production takes place under the nitrogen starvation period during the cell growth. Fifty Agrobacterium sp. were isolated from root nodules collected from different localities of Coimbatore and Palakkad and screened by Aniline Blue method for the production of curdlan. The species was confirmed as Agro-bacterium fabrum by 16S rRNA sequencing. The accession number given by GenBank is MF521602. Curdlan was produced using different carbon and nitrogen sources. The extraction was done by Sodium hydroxide: Acetic acid precipitation. 0.12 gm of crude curdlan was obtained from 1.2 gm/100 ml of biomass. The product was stored for further research.

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“…1A and 1C), which offers the immobilized cells immediate and sufficient contact to the surrounding aqueous medium and thus favors nutrient diffusion and oxygen transfer for cell growth [27,34]. Since numerous hydroxyl groups are present in lignocellulose and the main constituents of cell walls are hydrophilic polysaccharides, excessive hydrogen bonds between two surfaces result in another advantage of loofah sponge for cell adherence [26]. Comparing Figs.…”

Section: Cell Immobilizationmentioning

confidence: 99%

“…Under optimal conditions, the highest methyl ester yield was found to be 86.0% after a 72-h reaction, which is lower than ours (90.7%) at the end of the first batch reaction. This is mostly attributed to abundant water/oil interfaces generated by the high hydrophilicity of loofah sponge [26], which provided sufficient reaction sites.…”

Section: Reusability Of Lsp-immobilized Cellsmentioning

confidence: 99%

Biodiesel Production: Utilization of Loofah Sponge to Immobilize Rhizopus chinensis CGMCC #3.0232 Cells as a Whole-Cell Biocatalyst

He¹,

Xia²,

Wang³

et al. 2016

Journal of Microbiology and Biotechnology

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Rhizopus chinensis cells immobilized on loofah (Luffa cylindrica) sponges were used to produce biodiesel via the transesterification of soybean oil. In whole-cell immobilization, loofah sponge is considered to be a superior alternative to conventional biomass carriers because of its biodegradable and renewable properties. During cell cultivation, Rhizopus chinensis mycelia can spontaneously and firmly adhere to the surface of loofah sponge particles. The optimal conditions for processing 9.65 g soybean oil at 40°C and 180 rpm using a 3:1 methanol-to-oil molar ratio were found to be 8% cell addition and 3-10% water content (depending on the oil's weight). Under optimal conditions, an over 90% methyl ester yield was achieved after the first reaction batch. The operational stability of immobilized Rhizopus chinensis cells was assayed utilizing a 1:1 methanol-to-oil molar ratio, thus resulting in a 16.5-fold increase in half-life when compared with immobilized cells of the widely studied Rhizopus oryzae. These results suggest that transesterification of vegetable oil using Rhizopus chinensis whole cells immobilized onto loofah sponge is an effective approach for biodiesel production.

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Characterization of curdlan produced by Agrobacterium sp. IFO 13140 cells immobilized in a loofa sponge matrix, and application of this biopolymer in the development of functional yogurt

Abstract: A large number of cells physically adhered to the surface of loofa sponge fibers, and its use as an immobilization matrix to produce curdlan was effective. The use of the produced curdlan in yogurt allowed the development of a more stable product. © 2015 Society of Chemical Industry.

Cited by 20 publications

References 39 publications

Effect of Gelation Temperature on the Molecular Structure and Physicochemical Properties of the Curdlan Matrix: Spectroscopic and Microscopic Analyses

Effect of Gelation Temperature on the Molecular Structure and Physicochemical Properties of the Curdlan Matrix: Spectroscopic and Microscopic Analyses

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Biodiesel Production: Utilization of Loofah Sponge to Immobilize Rhizopus chinensis CGMCC #3.0232 Cells as a Whole-Cell Biocatalyst

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