Characterization of cytotoxin-producing Aeromonas caviae (strain HT10) isolated from a sulfur spring in Orissa, India

Deb

International Journal of Systematic and Evolutionary Microbiology

2021

A novel strain of a member of the genus Acinetobacter, strain PS-1T, was isolated from the skin of fresh water pufferfish (Tetraodon cutcutia) collected from Mahanadi River, India. Cells were Gram-stain-negative, aerobic, coccoid and non-motile. The predominant polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE) and phospholipid (PL) and the cell wall sugars were glucose, galactose and ribose. The major cellular fatty acids of PS-1T were C18 : 1ω9c (30.67 %), C16 : 1ω7c (19.54 %), C16 : 0 (15.87 %), C12 : 0 (7.35 %) and C12 : 0 3-OH (6.77 %). The genome size was 3.5 Mbp and the DNA G+C content was 41.97 %. Gene ontology study revealed that the major fraction of genes were associated with biological processes (53.99 %) followed by molecular function (30.42 %) and cellular components (15.58 %). Comparisons of 16S rRNA gene sequences revealed 97.94–97.05 % sequence similarity with the closely related type strains of species of the genus Acinetobacter . The average nucleotide identity (ANI) and average amino acid identity (AAI) of PS-1T with reference strains of species of the genus Acinetobacter with validly published names were bellow 95–96 and the corresponding in-silico DNA–DNA hybridization (DDH) values were below 70 %. A phylogenomic tree based on core genome analysis supported these results. Genotypic and phenotypic characteristics of PS-1T indicate that the strain represents a novel species of the genus Acinetobacter and the name Acinetobacter kanungonis sp. nov. is proposed. The type strain is PS-1T (=JCM 34131T=NCIMB 15260T)

Section: Phenotypic Characterizationmentioning

confidence: 99%

Description of Acinetobacter kanungonis sp. nov., based on phylogenomic analysis

Deb

International Journal of Systematic and Evolutionary Microbiology

2021

“…The wide diversity of aeromonads' habitat can clearly be seen by the recently isolated cytotoxic and hemolytic strain of A. caviae from an explored sulfur spring in Orissa, India [21]. The growth temperature of this strain ranged from 12 o to 43 o C and the optimum was 30 o C.…”

Section: Habitat and Bacteriologymentioning

confidence: 99%

Aeromonas-Associated Infections in Developing Countries

Ghenghesh

Ahmed²,

El-Khalek³

et al. 2008

J Infect Dev Ctries

Although their role in gastroenteritis is controversial, Aeromonas species are recognized as etiological agents of a wide spectrum of diseases in man and animals. In developing countries, potentially pathogenic Aeromonas sp. are very common in drinking water and in different types of foods, particularly seafood. Several food-borne and water-borne outbreaks as well nosocomial outbreaks associated with aeromonads have been reported. Significant association of Aeromonas sp. with diarrhoea in children has been reported from several countries. These organisms are important causes of skin and soft-tissue infections and aspiration pneumonia following contact with water and after floods. High incidence of antimicrobial resistance, including to third-generation cephalosporins and the fluoroquinolones, is found among Aeromonas sp. isolated from clinical sources in some developing countries in Asia. Isolating and identifying Aeromonas sp. to genus level is simple and requires resources that are available in most microbiology laboratories for processing common enteric bacteria. The present review will cover the epidemiology, clinical syndromes, low-cost diagnostic methods, and antimicrobial resistance and treatment of Aeromonas infections in developing countries.

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

“…The 16S rRNA gene of strain S10 T was amplified as described previously (Das et al, 1996;Patra et al, 2007). The 16S rRNA gene sequence (1440 nt) thus obtained was compared with those in GenBank and the Ribosomal Database Project (Maidak et al, 1997) after BLAST searches (Altschul et al, 1997).…”

mentioning

confidence: 99%

Thiomonas bhubaneswarensis sp. nov., an obligately mixotrophic, moderately thermophilic, thiosulfate-oxidizing bacterium

Panda

Jyoti

Bhadra

et al. 2009

A novel aerobic, obligately mixotrophic, moderately thermophilic, thiosulfate-oxidizing bacterium, S10 T , was isolated from hot-spring sediment samples collected from Atri, Bhubaneswar, India. The cells of this isolate stained Gram-negative and were strictly aerobic, non-sporulating, rodshaped and motile with a single polar flagellum. Strain S10 T was positive for oxidase and catalase activities. It was capable of utilizing thiosulfate under mixotrophic growth conditions. Mixotrophic growth was observed at pH 6.0-8.5 and 25-45 6C; optimum growth occurred at pH 7.5-8.0 and 30-37 6C. The major cellular fatty acids were C 12 : 0 3-OH, C 16 : 1 v7c, C 16 : 0 , C 17 : 0 cyclo, C 18 : 1 v7c and C 19 : 0 cyclo v8c. The DNA G+C content of strain S10 T was 64.8 mol%. 16S rRNA gene sequence analysis indicated that the bacterium clustered within the radiation of the genus Thiomonas and showed 98.0 % similarity with Thiomonas perometabolis ATCC 23370 T and Thiomonas intermedia ATCC 15466 T . However, DNA-DNA reassociation values of strain S10 T with Thiomonas perometabolis JCM 20426 T and Thiomonas intermedia JCM 20425 T , its nearest phylogenetic relatives, were 46 and 39 %, respectively. On the basis of phenotypic, physiological and chemotaxonomic properties, 16S rRNA gene sequence analysis and DNA-DNA reassociation studies, it is proposed that strain S10 T represents a novel species of the genus Thiomonas, Thiomonas bhubaneswarensis sp. nov.; the type strain is S10 T (5DSM 18181 T 5JCM 14806 T ).