Characterization of DDX4 Gene Expression in Human Cases with Non-Obstructive Azoospermia and in Sterile and Fertile Mice

Azizi, Hossein; Tabar, Amirreza Niazi; Mohammadi, Atiyeh; Skutella, Thomas

doi:10.18502/jri.v22i2.5793

Cited by 9 publications

(6 citation statements)

References 40 publications

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“…The expression of cell markers, including Oct4 [ 26 ], Sox-9 [ 27 ], 3β-HSD [ 28 ], and DDX4 [ 29 ], showed an intact ultrastructure containing undifferentiated spermatogonia, Sertoli cells, Leydig cells, and germ cells (arrow), respectively, in both the fresh control and SSV group. Intact Leydig cells, Sertoli cells, and undifferentiated spermatogonia represent early spermatogenesis without mature sperm in both groups before transplantation ( Figure 5 ).…”

Section: Resultsmentioning

confidence: 99%

Tracking Immature Testicular Tissue after Vitrification In Vitro and In Vivo for Pre-Pubertal Fertility Preservation: A Translational Transgenic Mouse Model

Huang

Liu

et al. 2022

IJMS

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Pediatric cancer survivors experiencing gonadotoxic chemoradiation therapy may encounter subfertility or permanent infertility. However, previous studies of cryopreservation of immature testicular tissue (ITT) have mainly been limited to in vitro studies. In this study, we aim to evaluate in vitro and in vivo bioluminescence imaging (BLI) for solid surface-vitrified (SSV) ITT grafts until adulthood. The donors and recipients were transgenic and wild-type mice, respectively, with fresh ITT grafts used as the control group. In our study, the frozen ITT grafts remained intact as shown in the BLI, scanning electron microscopy (SEM) and immunohistochemistry (IHC) analyses. Graft survival was analyzed by BLI on days 1, 2, 5, 7, and 31 after transplantation. The signals decreased by quantum yield between days 2 and 5 in both groups, but gradually increased afterwards until day 31, which were significantly stronger than day 1 after transplantation (p = 0.008). The differences between the two groups were constantly insignificant, suggesting that both fresh and SSV ITT can survive, accompanied by spermatogenesis, until adulthood. The ITT in both groups presented similar BLI intensity and intact cells and ultrastructures for spermatogenesis. This translational model demonstrates the great potential of SSV for ITT in pre-pubertal male fertility preservation.

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Section: Resultsmentioning

confidence: 99%

Tracking Immature Testicular Tissue after Vitrification In Vitro and In Vivo for Pre-Pubertal Fertility Preservation: A Translational Transgenic Mouse Model

Huang

Liu

et al. 2022

IJMS

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“…The absence of all primary antibodies in the sample served as a negative control for all indicators. A confocal Zeiss LSM 700 microscope was used to study the labeled cells, and pictures were captured using a Zeiss LSM-TPMT camera 30 .…”

Section: Methodsmentioning

confidence: 99%

“…In addition, we used an agarose gel in Tris–acetate buffer EDTA. The gel was stained with ethidium bromide at 0.75 g/mL and photographed using a UV transilluminator (INTAS, Germany) 30 , 31 .…”

Section: Methodsmentioning

confidence: 99%

VASA protein and gene expression analysis of human non-obstructive azoospermia and normal by immunohistochemistry, immunocytochemistry, and bioinformatics analysis

Amirian

Azizi

Karoii

et al. 2022

Sci Rep

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VASA, also known as DDX4, is a member of the DEAD-box proteins and an RNA binding protein with an ATP-dependent RNA helicase. The VASA gene expression, which is required for human germ cell development, may lead to infertility. Immunocytochemistry and immunohistochemistry were used to examine the expression of VASA protein in the human testis sections of azoospermic patients, in-vitro and in-silico models. Some studies of fertile humans showed VASA expression in the basal and adluminal compartments of seminiferous tubules. Our Immunocytochemistry and immunohistochemistry in infertile humans showed expression of VASA in the luminal compartments of the seminiferous tubule. The immunohistochemical analysis of three human cases with different levels of non-obstructive azoospermia revealed a higher expression of VASA-positive cells. For this purpose, Enrichr and Shiny Gene Ontology databases were used for pathway enrichment analysis and gene ontology. STRING and Cytoscape online evaluation were applied to predict proteins' functional and molecular interactions and performed to recognize the master genes, respectively. According to the obtained results, the main molecular functions of the up-regulated and downregulated genes include the meiotic cell cycle, RNA binding, and differentiation. STRING and Cytoscape analyses presented seven genes, i.e., DDX5, TNP2, DDX3Y, TDRD6, SOHL2, DDX31, and SYCP3, as the hub genes involved in infertility with VASA co-function and protein–protein interaction. Our findings suggest that VASA and its interacting hub proteins could help determine the pathophysiology of germ cell abnormalities and infertility.

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“…ARID4B is expressed in the testes and serves a role in the blood-testis barrier in mice 29 . DDX4 is typically expressed in spermatocytes and spermatids 30 .…”

Section: Genes Involved In Reproductionmentioning

confidence: 99%

Strong signatures of selection on genes underlying core reinforcement mechanisms in speciating desert tortoises

Baty,

Araya-Donoso,

Paulsen

et al. 2024

Preprint

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Genomic reinforcement and differential ecological adaptation are thought to be fundamental mechanisms of speciation. In this study we investigate the genomic basis of adaptation and reinforcement between two desert tortoise species of North America that occupy desert habitats with differing seasonal rainfall patterns and have considerable behavioral and reproductive differences yet maintain a narrow hybrid zone. We generated a chromosome-scale reference genome for Gopherus morafkai and performed analysis of synteny, genes under positive selection, and environmental niche modeling. Results show extensive positive selection (422 genes) including related to eye development and function that may relate to environmental differences, as well as prezygotic isolation mechanisms such as sperm-egg recognition, and postzygotic reinforcement mechanisms such as the spindle assembly checkpoint, and sister chromatid pairing. Together, results offer strong genetic support for the role of these classic processes in shaping reproductive isolation and lineage divergence of speciating tortoises.

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Characterization of DDX4 Gene Expression in Human Cases with Non-Obstructive Azoospermia and in Sterile and Fertile Mice

Cited by 9 publications

References 40 publications

Tracking Immature Testicular Tissue after Vitrification In Vitro and In Vivo for Pre-Pubertal Fertility Preservation: A Translational Transgenic Mouse Model

Tracking Immature Testicular Tissue after Vitrification In Vitro and In Vivo for Pre-Pubertal Fertility Preservation: A Translational Transgenic Mouse Model

VASA protein and gene expression analysis of human non-obstructive azoospermia and normal by immunohistochemistry, immunocytochemistry, and bioinformatics analysis

Strong signatures of selection on genes underlying core reinforcement mechanisms in speciating desert tortoises

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