Characterization of decellularized COPD lung matrices using mass spectrometry proteomics

Uhl, Franziska E.; Zvarova, Barbora; Ahlers, Bethany A.; Hood, C. Ian; Deng, Bin; Lam, Ying‐Wai; Beatman, Erica L.; Schweitzer, Kelly S.; Petrache, Irina; Weiss, Daniel J.; Wagner, Darcy E.

doi:10.1183/13993003.congress-2018.lsc-1085

Cited by 1 publication

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“…Early proteomic studies ( Booth et al, 2012 ; Uhl et al, 2018 ) illustrated the retention of many components of the lung matrisome in decellularised scaffolds from control and diseased lung samples. A recent study from the team in the Weiss lab ( Hoffman et al, 2023 ), has elegantly shown that the components of the ECM are specific for different compartments within the lung (airways, alveolus, blood vessels), and that these change during chronic lung disease.…”

Section: The Possibilities With Lung Ecm-derived Hydrogelsmentioning

confidence: 99%

Current possibilities and future opportunities provided by three-dimensional lung ECM-derived hydrogels

Nizamoglu

Burgess

2023

Front. Pharmacol.

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Disruption of the complex interplay between cells and extracellular matrix (ECM), the scaffold that provides support, biochemical and biomechanical cues, is emerging as a key element underlying lung diseases. We readily acknowledge that the lung is a flexible, relatively soft tissue that is three dimensional (3D) in structure, hence a need exists to develop in vitro model systems that reflect these properties. Lung ECM-derived hydrogels have recently emerged as a model system that mimics native lung physiology; they contain most of the plethora of biochemical components in native lung, as well as reflecting the biomechanics of native tissue. Research investigating the contribution of cell:matrix interactions to acute and chronic lung diseases has begun adopting these models but has yet to harness their full potential. This perspective article provides insight about the latest advances in the development, modification, characterization and utilization of lung ECM-derived hydrogels. We highlight some opportunities for expanding research incorporating lung ECM-derived hydrogels and potential improvements for the current approaches. Expanding the capabilities of investigations using lung ECM-derived hydrogels is positioned at a cross roads of disciplines, the path to new and innovative strategies for unravelling disease underlying mechanisms will benefit greatly from interdisciplinary approaches. While challenges need to be addressed before the maximum potential can be unlocked, with the rapid pace at which this field is evolving, we are close to a future where faster, more efficient and safer drug development targeting the disrupted 3D microenvironment is possible using lung ECM-derived hydrogels.

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Section: The Possibilities With Lung Ecm-derived Hydrogelsmentioning

confidence: 99%