Characterization of Dendritic Cells Subpopulations in Skin and Afferent Lymph in the Swine Model

Marquet, Florian; Bonneau, Michel; Pascale, Florentina; Urien, Céline; Kang, Chantal; Schwartz-Cornil, Isabelle; Bertho, Nicolas

doi:10.1371/journal.pone.0016320

Cited by 65 publications

(74 citation statements)

References 32 publications

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“…In pig and macaques, cDC1 were the only cells to strongly express CADM1. In macaques, cDC1 were also XCR1 positive and expressed CLEC9A and BATF3 at the RNA level; similar to human cDC1, they also strongly expressed CD205 and CD162 and were responsive to TLR3 triggering [75,76]. cDC that closely resemble cDC1 in terms of FLT3, CADM1, CD205, TLR3 and XCR1 expression, were also identified for the first time in a non-mammalian species, the chicken [77].…”

Section: Xcr1 + Cadm1 + Cdc1 Across Speciesmentioning

confidence: 77%

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Aligning bona fide dendritic cell populations across species

Dutertre

Wang

Ginhoux

2014

Cellular Immunology

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Section: Xcr1 + Cadm1 + Cdc1 Across Speciesmentioning

confidence: 77%

“…Very recently, DC sharing phenotypic, molecular and functional similarities to mouse, human and sheep cDC1 were defined in other species including macaques, pigs and chickens [75,76]. In pig and macaques, cDC1 were the only cells to strongly express CADM1.…”

Section: Xcr1 + Cadm1 + Cdc1 Across Speciesmentioning

confidence: 99%

Aligning bona fide dendritic cell populations across species

Dutertre

Wang

Ginhoux

2014

Cellular Immunology

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“…30 The scavenger receptors, CD163 and CD204, are also expressed by DC subsets in some species. 43,87 Expression patterns of CD163 and CD204 in normal canine DCs have not been reported. Of the markers mentioned, only CD11b is not assessable in formalin-fixed tissues.…”

Section: Histiocytic Diseases Of Interstitial DC and Macrophage Originmentioning

confidence: 99%

“…5 Macrophage scavenger receptors are present on subsets of interstitial DCs, so this could be one potential point of reconciliation of the divergent data. 43,87 Also, dendritic cells are located in the meninges and choroid plexi but not in the brain. 19 Hence, the apparent site of initiation of CNS HS does have a resident population of interstitial DCs from which HS could develop.…”

Section: Distinctive Histiocytic Sarcoma Syndromesmentioning

confidence: 99%

A Review of Histiocytic Diseases of Dogs and Cats

2014

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Histiocytic proliferative disorders are commonly observed in dogs and less often cats. Histiocytic disorders occur in most of the dendritic cell (DC) lineages. Canine cutaneous histiocytoma originates from Langerhans cells (LCs) indicated by expression of CD1a, CD11c/CD18, and E-cadherin. When histiocytomas occur as multiple lesions in skin with optional metastasis to lymph nodes and internal organs, the disease resembles cutaneous Langerhans cell histiocytosis of humans. Langerhans cell disorders do not occur in feline skin. Feline pulmonary LCH has been recognized as a cause of respiratory failure due to diffuse pulmonary infiltration by histiocytes, which express CD18 and E-cadherin and contain Birbeck's granules. In dogs and cats, histiocytic sarcomas (HS) arise from interstitial DCs that occur in most tissues of the body. Histiocytic sarcomas begin as localized lesions, which rapidly disseminate to many organs. Primary sites include spleen, lung, skin, brain (meninges), lymph node, bone marrow, and synovial tissues of limbs. An indolent form of localized HS, progressive histiocytosis, originates in the skin of cats. Hemophagocytic HS originates in splenic red pulp and bone marrow macrophages in dogs and cats. In dogs, histiocytes in hemophagocytic HS express CD11d/CD18, which is a leuko-integrin highly expressed by macrophages in splenic red pulp and bone marrow. Canine reactive histiocytic diseases, systemic histiocytosis (SH) and cutaneous histiocytosis, are complex inflammatory diseases with underlying immune dysregulation. The lesions are dominated by activated interstitial DCs and lymphocytes, which invade vessel walls and extend as vasocentric infiltrates in skin, lymph nodes, and internal organs (SH).

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“…Most systems used to investigate DC-T cell interactions rely on the isolation of monocytes or macrophages from blood or tissues (such as spleen or bone marrow) (63,71), followed by maturation with interleukin 4 (IL-4) and granulocyte-macrophage colony-stimulating factor (GM-CSF) or the harvesting of tissues followed by isolation of resident DC. Only the model of cannulation of lymphatic vessels provides biological material, including DC derived from anatomical sites used in vaccination (31,32,34,49,68). Various DC models have been used to identify MVA-DC interactions to try to understand rMVA-induced immunogenicity.…”

mentioning

confidence: 99%

Modified Vaccinia Virus Ankara-Based Vaccine Vectors Induce Apoptosis in Dendritic Cells Draining from the Skin via both the Extrinsic and Intrinsic Caspase Pathways, Preventing Efficient Antigen Presentation

Guzman¹,

Cubillos‐Zapata²,

Cottingham

et al. 2012

J Virol

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Dendritic cells (DC) are potent antigen-presenting cells and central to the induction of immune responses following infection or vaccination. The collection of DC migrating from peripheral tissues by cannulation of the afferent lymphatic vessels provides DC which can be used directly ex vivo without extensive in vitro manipulations. We have previously used bovine migrating DC to show that recombinant human adenovirus 5 vectors efficiently transduce afferent lymph migrating DEC-205 + CD11c + CD8 − DC (ALDC). We have also shown that recombinant modified vaccinia virus Ankara (MVA) infects ALDC in vitro , causing downregulation of costimulatory molecules, apoptosis, and cell death. We now show that in the bovine system, modified vaccinia virus Ankara-induced apoptosis in DC draining from the skin occurs soon after virus binding via the caspase 8 pathway and is not associated with viral gene expression. We also show that after virus entry, the caspase 9 pathway cascade is initiated. The magnitude of T cell responses to mycobacterial antigen 85A (Ag85A) expressed by recombinant MVA-infected ALDC is increased by blocking caspase-induced apoptosis. Apoptotic bodies generated by recombinant MVA (rMVA)-Ag85A-infected ALDC and containing Ag85A were phagocytosed by noninfected migrating ALDC expressing SIRPα via actin-dependent phagocytosis, and these ALDC in turn presented antigen. However, the addition of fresh ALDC to MVA-infected cultures did not improve on the magnitude of the T cell responses; in contrast, these noninfected DC showed downregulation of major histocompatibility complex class II (MHC-II), CD40, CD80, and CD86. We also observed that MVA-infected ALDC promoted migration of DEC-205 + SIRPα + CD21 + DC as well as CD4 + and CD8 + T cells independently of caspase activation. These in vitro studies show that induction of apoptosis in DC by MVA vectors is detrimental to the subsequent induction of T cell responses.

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Characterization of Dendritic Cells Subpopulations in Skin and Afferent Lymph in the Swine Model

Cited by 65 publications

References 32 publications

Aligning bona fide dendritic cell populations across species

Aligning bona fide dendritic cell populations across species

A Review of Histiocytic Diseases of Dogs and Cats

Modified Vaccinia Virus Ankara-Based Vaccine Vectors Induce Apoptosis in Dendritic Cells Draining from the Skin via both the Extrinsic and Intrinsic Caspase Pathways, Preventing Efficient Antigen Presentation

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