Characterization of dexamethasone loaded collagen-chitosan sponge and in vitro release study

Alagha, Amjad; Nourallah, Abdulwahab; Hariri, Sahar

doi:10.1016/j.jddst.2019.101449

Cited by 19 publications

(8 citation statements)

References 57 publications

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“…One of the methods to improve its biophysical properties is preparing collagen-based materials by mixing them with other macromolecules. Namely, collagen can be blended with other proteins, such as silk fibroin or elastin [ 57 , 58 ], but also with polysaccharides, including hyaluronic acid [ 59 ], chitosan [ 60 ], or sodium alginate [ 61 ]. Following an earlier study of Kaczmarek et al [ 62 ], herein, we investigated different scaffolds based on collagen derived from rat tail tendon or from Salmo salar fish skin mixed with chitosan.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Polymeric Matrix Loaded with Melatonin for Wound Dressing

Kaczmarek-Szczepańska

Ostrowska

Kozłowska

et al. 2021

IJMS

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The development of scaffolds mimicking the extracellular matrix containing bioactive substances has great potential in tissue engineering and wound healing applications. This study investigates melatonin—a methoxyindole present in almost all biological systems. Melatonin is a bioregulator in terms of its potential clinical importance for future therapies of cutaneous diseases. Mammalian skin is not only a prominent melatonin target, but also produces and rapidly metabolizes the multifunctional methoxyindole to biologically active metabolites. In our methodology, chitosan/collagen (CTS/Coll)-contained biomaterials are blended with melatonin at different doses to fabricate biomimetic hybrid scaffolds. We use rat tail tendon- and Salmo salar fish skin-derived collagens to assess biophysical and cellular properties by (i) Fourier transform infrared spectroscopy—attenuated total reflectance (FTIR–ATR), (ii) thermogravimetric analysis (TG), (iii) scanning electron microscope (SEM), and (iv) proliferation ratio of cutaneous cells in vitro. Our results indicate that melatonin itself does not negatively affect biophysical properties of melatonin-immobilized hybrid scaffolds, but it induces a pronounced elevation of cell viability within human epidermal keratinocytes (NHEK), dermal fibroblasts (NHDF), and reference melanoma cells. These results demonstrate that this indoleamine accelerates re-epithelialization. This delivery is a promising technique for additional explorations in future dermatotherapy and protective skin medicine.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Polymeric Matrix Loaded with Melatonin for Wound Dressing

Kaczmarek-Szczepańska

Ostrowska

Kozłowska

et al. 2021

IJMS

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“…Collagen sponges with pore sizes ranging from 63 μm to 150 μm may be suitable for the adhesion and growth of fibroblasts. 26 Images of DTSCS (Figure 2(a)) and STSCS (Figure 2(b)) show that these freeze-dried sponges were white and with a surface that was flat and uniform, by direct observation. At a magnification of 200×, the structure of DTSCS (Figure 2(c)) indicated dense collagen fibers distributed in strips.…”

Section: Resultsmentioning

confidence: 98%

Biocompatibility, hemostatic properties, and wound healing evaluation of tilapia skin collagen sponges

Wang

Yang

Wang

et al. 2020

Journal of Bioactive and Compatible Polymers

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Dialyzed tilapia skin collagen sponge (DTSCS) and self-assembled tilapia skin collagen sponge (STSCS) were prepared by freeze-drying. The raw components used in the fabrication of DTSCS and STSCS were separated and purified from tilapia fish skin. It is anticipated that these collagen sponges could be developed into medical dressings for hemostasis and wound healing. The aim of the present research was to explore the possibility of DTSCS and STSCS as medical dressings and compare their differences by scanning electron microscopy (SEM), water absorption measurement, differential scanning calorimetry (DSC), measurement of porosity, cytotoxicity, hemolysis, in vivo biocompatibility, and evaluation of hemostatic performance and wound healing. The results indicate that DTSCS and STSCS are suitable materials for use in medical applications with a loose and porous structure, high water absorption, high porosity, and high thermal stability. The materials also displayed good biocompatibility, including excellent blood compatibility, a lack of cytotoxicity, with no apparent rejection following implantation. STSCS exhibited rapid hemostasis and promoted healing, with slightly greater efficacy than DTSCS. The hemostatic properties and promotion of healing in DTSCS was similar to that of commercial bovine collagen sponge. Therefore, DTSCS and STSCS both represented excellent potential candidate materials for use as hemostatic agents and wound dressings.

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“…Drug release study from the sponge shown that the release of drug from the hybrid sponge was faster than the chitosan sponge, which ended in 16h, and was slower than the collagen sponge which completed within 5h. The formulation based on collagen-chitosan (1:1 wt %) blend was able to control drug release within 10h (82) . Thus, the dexamethasone loaded -blend of high molecular weight chitosan and collagen, to form a bio-sponge hydrogel for DPC, will have advantages of both collagen and chitosan properties, in addition to the properties of prolonging released dexamethasone, which is about 30 times more effective than cortisone (83) .…”

Section: Discussionmentioning

confidence: 99%

Dexamethasone - loaded polymeric porous sponge as a hard tissue regeneration agent

Alagha¹

2020

Preprint

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Background : The aim of this study is to achieve the principles of tissue engineering using biopolymers to be applied in the field of vital endodontic treatment with the aim of stimulating stem cells and engineering and regeneration of dentin tissue. the blend was loaded with the steroidal antiinflammatory drug, dexamethasone, and the porous drug-loaded bio-sponge was produced by lyophilization. Bio-sponge, as a direct pulp capping agent, was histologically studied compared to calcium hydroxide Ca(OH)2 in an animal experiment.Results: The results indicated the effectiveness of the bio-sponge as a direct pulp agent, where the dentin bridge was formed faster than Ca(OH)2 treated samples,. There was no inflammatory response in the pulp tissue throughout the follow-up period.Conclusions : The porous bio-sponge loaded with dexamethasone with a neutral pH resulted in enhancement the odontoblast differentiation from stem cells, resulted in the formation of a renewed dentin bridge without the slightest inflammatory response in the pulp. aminoglycan GAG (13) . ECM is responsible for cellular metabolism and the forming of new tissues since it gives the mechanical and biochemical properties for the formed tissue (14) . ECM plays a crucial role in regulating the expression of the differentiated phenotype and in supporting both migration and proliferation of fibroblast cells (15) .Chitin, the most abundant polysaccharide in nature after cellulose, is synthesized by an enormous number of living organisms, presents in the exoskeleton of crustaceans, insects and fungal cell walls (16) . Chitin consists of a saccharide backbone with β-1,4-linked glucosamine units and characterized by a high availability of acetyl group (17) . Chitin has a cellulose-like structure with the difference of hydroxyl-group replacement by (-NHCOCH 3 ) at the C 2 site (18) . Chitosan is the main derivative of chitin and derived by alkaline deacetylation of chitin (19) . The availability of functional groups allows chemical modifications, making it possible to load and release drugs from chitosan (20) .Chitosan scaffolds with an interconnected porous structure have been shown to be easily manufactured by lyophilization of chitosan solution (21) , and have been widely studied in the bone tissue engineering, and it has been shown that it promotes osteoblast cells growth and deposition of the mineralized matrix by cells (22) . The addition of chitosan molecules to the human bone marrow transplantation medium stimulates bone deposition by promoting cell differentiation (23) . It has been suggested that chitosan enhanced bone formation in vivo through indirect mechanisms (23) , and it can be used as a standard GAG in tissue regeneration processes (24) .Collagen is the most abundant protein in the body and it is the major component in body tissues, it forms also about 30% of total proteins in mammals (25) . About 28 types of collagen have been identified (26) , among which, the type-I collagen is the prevalent type and it is the main component of the ECM (27) ....

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Characterization of dexamethasone loaded collagen-chitosan sponge and in vitro release study

Cited by 19 publications

References 57 publications

Evaluation of Polymeric Matrix Loaded with Melatonin for Wound Dressing

Evaluation of Polymeric Matrix Loaded with Melatonin for Wound Dressing

Biocompatibility, hemostatic properties, and wound healing evaluation of tilapia skin collagen sponges

Dexamethasone - loaded polymeric porous sponge as a hard tissue regeneration agent

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