Characterization of Diarrheagenic Escherichia coli Isolated in Organic Waste Products (Cattle Fecal Matter, Manure and, Slurry) from Cattle’s Markets in Ouagadougou, Burkina Faso

Abstract

Cattle farming can promote diarrheal disease transmission through waste, effluents or cattle fecal matter. The study aims to characterize the diarrheagenic Escherichia coli (DEC) isolated from cattle feces, manure in the composting process and slurry, collected from four cattle markets in Ouagadougou. A total of 585 samples (340 cattle feces, 200 slurries and 45 manures in the composting process) were collected from the four cattle markets between May 2015 and May 2016. A multiplex Polymerase Chain Reaction (PCR), namely 16-plex PCR, was used to screen simultaneously the virulence genes specific for shiga toxin-producing E. coli (STEC), enteropathogenic E. coli (EPEC), enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC) and enteroaggregative E. coli (EAEC). DEC was detected in 10.76% of samples. ETEC was the most prevalent (9.91%). STEC and EAEC have been observed with the same rate (0.51%). ETEC were detected in 12.64% of cattle feces, in 6.66% of manure in the composting process and in 5% of slurry. STEC were detected in 0.58% of cattle feces and in 2.22% of manure in the composting process. EAEC was detected only in 1% of slurry and in 2.22% of manure in the composting process. ETEC strains were identified based on estIa gene and/or estIb gene and/or elt gene amplification. Of the 58 ETEC, 10.34% contained astA, 17.24% contained elt, 3.44% contained estIa and 79.31% contained estIb. The two positive EAEC strains contained only the aggR gene, and the third was positive only for the pic gene. The results show that effluent from cattle markets could contribute to the spreading of DEC in the environment in Burkina Faso.

Antibiotic Resistance Pattern of Mastitis Causing Escherichia Coli Toxinotypes

“…Total eight (100%) isolates showed the presence of aggR gene, followed by five (62%) est gene, five (62%) elt gene, three (37.5%) eae gene and two (25%) Asp gene, respectively. Bako et al (2017) detected est, elt and aggR genes which was in accordance to the present study. High prevalence of these genes indicated the improper management of the effluents and spread of E. coli from humans to animals through environment and vice versa.…”

Characterization of Diarrheagenic Escherichia Coli Strains Isolated from Slaughtered Sheep in México

“…In Kenia, it was also reported as the third most frequent DEC (10%) in slaughtered goats, while an investigation in Mexico rated as the second most frequent in bovines, the same as in Burkina Faso (4%) (Njoroge et al 2013;Navarro et al 2018;Kagambéga et al 2012). Previous reports about ETEC have been described in bovines with/without diarrhea in Brazil, Vietnam (with the same number of isolates as this work), and Burkina Faso (De Moura et al 2012;Nguyen et al 2011;Bako et al 2017). There is a lack of information regarding this pathotype in slaughtered sheep in Mexico.…”

Detection of Diarrheagenic Escherichia Coli in Human Diarrheic Stool and Drinking Water Samples in Ouagadougou, Burkina Faso.

“…In Burkina, previous studies have reported the detection of ETEC in grilled chickens, cow dung and organic manure in Burkina Faso (Bako et al, 2017;Kambire et al, 2017;Somda et al, 2018). The similarity of pathovars found in human stool samples, drinking water, and in cow dung in Burkina Faso could be because the aquatic environments are contaminated with animal fecal droppings.…”