Characterization of diazotrophic root endophytes in Chinese silvergrass (Miscanthus sinensis)

Li, Yongbin; Yang, Rui; Häggblom, Max M.; Li, Mengyan; Guo, Lifang; Li, Baoqin; Cao, Zhiguo; Solemani, Mohsen; Chen, Zheng; Xu, Zhimin; Gao, Wenlong; Yan, Bei; Sun, Weimin

doi:10.1186/s40168-022-01379-9

Cited by 44 publications

(22 citation statements)

References 69 publications

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“…Pseudomonas has been shown to suppress plant pathogens and promote plant growth ( Tao et al., 2020 ). In addition, Pseudomonas has a strong N fixation capacity ( Li et al., 2022b ) and can provide N for P.communis , which may also be an important reason why P.communis can adapt to N-deprived habitat. Both the Cyclobacteriaceae family and MND1 genera were also found to promote plant growth ( Li et al., 2020 ; Cheng et al., 2022 ), which is consistent with our study.…”

Section: Discussionmentioning

confidence: 99%

Plants changed the response of bacterial community to the nitrogen and phosphorus addition ratio

Zhang

Sun

et al. 2023

Front. Plant Sci.

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IntroductionHuman activities have increased the nitrogen (N) and phosphorus (P) supply ratio of the natural ecosystem, which affects the growth of plants and the circulation of soil nutrients. However, the effect of the N and P supply ratio and the effect of plant on the soil microbial community are still unclear.MethodsIn this study, 16s rRNA sequencing was used to characterize the response of bacterial communities in Phragmites communis (P.communis) rhizosphere and non-rhizosphere soil to N and P addition ratio.ResultsThe results showed that the a-diversity of the P.communis rhizosphere soil bacterial community increased with increasing N and P addition ratio, which was caused by the increased salt and microbially available C content by the N and P ratio. N and P addition ratio decreased the pH of non-rhizosphere soil, which consequently decreased the a-diversity of the bacterial community. With increasing N and P addition ratio, the relative abundance of Proteobacteria and Bacteroidetes increased, while that of Actinobacteria and Acidobacteria decreased, which reflected the trophic strategy of the bacterial community. The bacterial community composition of the non-rhizosphere soil was significantly affected by salt, pH and total carbon (TC) content. Salt limited the relative abundance of Actinobacteria, and increased the relative abundance of Bacteroidetes. The symbiotic network of the rhizosphere soil bacterial community had lower robustness. This is attributed to the greater selective effect of plants on the bacterial community influenced by nutrient addition.DiscussionPlants played a regulatory role in the process of N and P addition affecting the bacterial community, and nutrient uptake by the root system reduced the negative impact of N and P addition on the bacterial community. The variations in the rhizosphere soil bacterial community were mainly caused by the response of the plant to the N and P addition ratio.

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Section: Discussionmentioning

confidence: 99%

Plants changed the response of bacterial community to the nitrogen and phosphorus addition ratio

Zhang

Sun

et al. 2023

Front. Plant Sci.

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“…50 DNA-SIP Assay. DNA-SIP assay was established to identify bacteria involved in phototrophic BNF using 15 N 2 or 14 N 2 . Samples from BL of FK were incubated in N-and organic Cfree broth.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Phototrophic Nitrogen Fixation, a Neglected Biogeochemical Process in Mine Tailings?

Li,

Sun,

Yang

et al. 2024

Environ. Sci. Technol.

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Biological nitrogen fixation (BNF) has important ecological significance in mine tailing by contributing to the initial accumulation of nitrogen. In addition to chemolithotrophic and heterotrophic BNF, light may also fuel BNF in oligotrophic mine tailings. However, knowledge regarding the occurrence and ecological significance of this biogeochemical process in mine tailings remains ambiguous. The current study observed phototrophic BNF in enrichment cultures established from three primary successional stages (i.e., original tailings, biological crusts, and pioneer plants) of tailings. Notably, phototrophic BNF in tailings may be more active at vegetation stages (i.e., biological crusts and pioneering plants) than in bare tailings. DNA-stable isotope probing identified Roseomonas species as potential aerobic anoxygenic phototrophs responsible for phototrophic BNF. Furthermore, metagenomic binning as well as genome mining revealed that Roseomonas spp. contained essential genes involved in nitrogen fixation, anoxygenic photosynthesis, and carbon fixation, suggesting their genetic potential to mediate phototrophic BNF. A causal inference framework equipped with the structural causal model suggested that the enrichment of putative phototrophic diazotrophic Roseomonas may contribute to an elevated total nitrogen content during primary succession in these mine tailings. Collectively, our findings suggest that phototrophic diazotrophs may play important roles in nutrient accumulation and hold the potential to facilitate ecological succession in tailings.

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“…The Sb-dependent BNF activity assay includes nitrogenase activity measurements and Sb speciation (Sb(V) and Sb(III)) analysis. Acetylene reduction assay (ARA) was applied to analyze nitrogenase activity. − Briefly, 60 mL sterile bottles were filled with 30 mL slurries of 1 g soil or tailing samples. Half of the experimental bottles were supplemented with 1 mM Sb(III) and 3 mM NaHCO 3 (Sb treatment) or 3 mM NaHCO 3 as a control treatment (No-Sb control).…”

Section: Materials and Methodsmentioning

confidence: 99%

Chemolithotrophic Biological Nitrogen Fixation Fueled by Antimonite Oxidation May Be Widespread in Sb-Contaminated Habitats

Guo

Yang

et al. 2022

Environ. Sci. Technol.

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Nitrogen (N) deficiency in mining-contaminated habitats usually hinders plant growth and thus hampers tailing revegetation. Biological N fixation (BNF) is an essential biogeochemical process that contributes to the initial accumulation of N in oligotrophic mining-contaminated regions. Previous studies reported that chemolithotrophic rather than heterotrophic diazotrophs frequently dominated in the mining-contaminated regions. Chemolithotrophic diazotrophs may utilize elements abundant in such habitats (e.g., sulfur (S), arsenic (As), and antimony (Sb)) as electron donors to fix N 2 . BNF fueled by the oxidation of S and As has been detected in previous studies. However, BNF fueled by Sb(III) oxidation (Sb-dependent BNF) has never been reported. The current study observed the presence of Sb-dependent BNF in slurries inoculated from Sb-contaminated habitats across the South China Sb belt, suggesting that Sb-dependent BNF may be widespread in this region. DNA-stable isotope probing identified bacteria associated with Rhodocyclaceae and Rhizobiaceae as putative microorganisms responsible for Sb-dependent BNF. Furthermore, metagenomicbinning demonstrated that Rhodocyclaceae and Rhizobiaceae contained essential genes involved in Sb(III) oxidation, N 2 fixation, and carbon fixation, suggesting their genetic potential for Sb-dependent BNF. In addition, meta-analysis indicated that these bacteria are widespread among Sb-contaminated habitats with different niche preferences: Rhodocyclaceae was enriched in river sediments and tailings, while Rhizobiaceae was enriched only in soils. This study may broaden our fundamental understanding of N fixation in Sbmining regions.

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Characterization of diazotrophic root endophytes in Chinese silvergrass (Miscanthus sinensis)

Cited by 44 publications

References 69 publications

Plants changed the response of bacterial community to the nitrogen and phosphorus addition ratio

Plants changed the response of bacterial community to the nitrogen and phosphorus addition ratio

Phototrophic Nitrogen Fixation, a Neglected Biogeochemical Process in Mine Tailings?

Chemolithotrophic Biological Nitrogen Fixation Fueled by Antimonite Oxidation May Be Widespread in Sb-Contaminated Habitats

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