Characterization of digestive enzymes during larval development of red drum (Sciaenops ocellatus)

Lazo, Juan Pablo; Mendoza, Roberto; Holt, G. Joan; Aguilera, Carlos; Arnold, C. R.

doi:10.1016/j.aquaculture.2007.01.043

Cited by 113 publications

(91 citation statements)

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“…Various protease isoforms were investigated in different aquatic species (Areekijseree et al 2004;Natalia et al 2004;Supannapong et al 2008;Thongprajukaew et al 2010a). For alkaline proteases, the optimal conditions of trypsin in male and female guppies were similar to those of other fish species, such as arowana (Natalia et al 2004), red drum S. ocellatus (Lazo et al 2007), walleye pollock Theragra chalcogramma (Kishimura et al 2008), and juvenile Siamese fighting fish (Thongprajukaew et al 2010b). Similarly, the optimal condition for chymotrypsin in guppies was also found in Atlantic salmon (Rungruangsak-Torrissen 2007) and arowana (Natalia et al 2004).…”

Section: Characterization Of Digestive Enzymesmentioning

confidence: 77%

“…The optimal condition for neutral lipase in guppies was similarly observed in red drum larvae Sciaenops ocellatus (Lazo et al 2007) and juvenile Siamese fighting fish (Thongprajukaew et al 2010b), while the acidic isoform of females was close to that of purified lipase, with optimal conditions of pH 5.5 at 35°C reported for Reba carp Cirrhinus reba (Islam et al 2009). Optimal conditions for sex-specific lipase isoforms were reported by Areekijseree et al (2002) and Thongprajukaew et al (2010b).…”

Section: Characterization Of Digestive Enzymesmentioning

confidence: 79%

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Effects of sex on characteristics and expression levels of digestive enzymes in the adult guppy Poecilia reticulata

Thongprajukaew

Kovitvadhi

2013

Zool. Stud.

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Background: Characteristics (pH 2 to 12 and temperature of 25°C to 80°C) and expression levels of the main digestive enzymes, including amylase, lipase, total protease, trypsin, and chymotrypsin, were investigated in adult male and female guppies Poecilia reticulata. A completely randomized design was used during this experiment. Results: Digestive enzyme studies indicated that sex had a direct effect on characteristic profiles (n = 3) and expression levels (n = 10) of all digestive enzymes. The most suitable conditions for studying digestive enzymes in general were pH 2 at 50°C for acidic amylase, pH 7 at 55°C for neutral amylase, pH 11 at 50°C for alkaline amylase, pH 7 at 30°C to 35°C for lipase, pH 2 at 55°C for acidic protease, pH 9 at 45°C for alkaline protease, pH 8 at 50°C for trypsin, and pH 9 at 50°C for chymotrypsin, regardless of sex. Specific activities of amylase, total protease, trypsin, and chymotrypsin were higher in females than males (p < 0.001), while the specific activity of lipase was higher in males than females. These findings were correlated with the higher growth capacity of females than males (except for lipase), as indicated by differences in body weight (p < 0.001), length (p < 0.001), and the activity ratio of trypsin (T) to chymotrypsin (C), i.e., the T/C ratio (p < 0.007).

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Section: Characterization Of Digestive Enzymesmentioning

confidence: 77%

Section: Characterization Of Digestive Enzymesmentioning

confidence: 79%

Effects of sex on characteristics and expression levels of digestive enzymes in the adult guppy Poecilia reticulata

Thongprajukaew

Kovitvadhi

2013

Zool. Stud.

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“…The trypsin band was affected by TLCK; SBT1 inhibited the trypsin and chymotrypsin bands, showing that trypsin inhibitors equally affect chymotrypsin activity, which was similar to the results of Alarcón, Díaz, Moyano, & Abellan (2001), where these inhibitors inhibited the band with molecular weights of 20 and 30 kDa in yellow snapper (Lutjanus argentiventris) and Pacific dog snapper (Lutjanus novemfasciatus). Lazo, Mendoza, Holt, Aguilera, & Arnold (2007), reported trypsin with molecular weights of 23 and 68 kDa and chymotrypsin with 25 kDa in S. ocellatus. Meanwhile, Natalia, Hashim, Ali, & Chong (2004), found trypsin and chymotrypsin with molecular weights of 32.3 kDa and 42.1 kDa, respectively, in S. formosus.…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, the protease activity could be affected by the quality of the ingredients composing the formula (Lazzari et al 2010;Costa et al 2011). Considering the above, the secretion of digestive enzymes depends on the physicochemical characteristics of the internal environmental, the quality and quantity of nutrients, and the presence of inhibitors in the feed (Lazo, Mendoza, Holt, Aguilera, & Arnold, 2007;Murashita et al 2015). In this sense, the studies on protease characterization allow us to understand the capacity of fish to hydrolyze and metabolize proteins, which are necessary for adequate growth and development (Ma et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Partial Characterization of Digestive Proteases in the Common Snook Centropomus undecimalis

Concha‐Frías¹,

Álvarez‐González²,

Gaxiola³

et al. 2016

IJB

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Common snook (Centropomus undecimalis) is a marine species with high aquaculture potential; although its digestive physiology is still unknown and knowledge of that could allow the development of a balanced feed for commercial culture of this fish. The objective of this study was to partially characterize the digestive proteases in C. undecimalis using electrophoretic and biochemical techniques. A total of 50 wild snook juveniles were used to determine the optimal values of pH stability and temperature as well as the effect of inhibitors on digestive, gastric and intestinal proteases. The optimal pH for gastric proteases was obtained to be 2 with stability obtained between 2 and 8; the optimal temperature was detected at 75ºC for in vitro test, and the thermal stability was between 25 and 45ºC. Intestinal proteases showed two peaks of activity at a pH of 7 and 11; meanwhile, the greatest stability was found between a pH of 4 and 10; the optimal temperature was at 65ºC, and the greatest stability was detected between 35 and 45ºC. Up to 86% of the gastric protease activity was inhibited by pepstatin A; meanwhile, the intestinal proteases TPCK, TLCK, 1-10 Phenanthroline, SBT1, EDTA, PMSF and ovalbumin reduced the activity by 17%, 68%, 85%, 41%, 40.5%, 60% and 59%, respectively.

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“…It is well known that the secretion of trypsin is known to occur in response to food ingestion and in larval pancreatic tissue, most of the trypsin is present as an enzymatically inactive trypsinogen, whereas most of the trypsin in the intestinal tract is enzymatically active (Ueberschär, 1993;1995). In addition to this, it is reported that recent results indicate an increasing importance of chymotrypsin relative to trypsin in larval fish, and at times chymotryptic activity may exceed tryptic activity (Lazo et al, 2007).…”

Section: Introductionmentioning

confidence: 74%

Untitled

Kamacı

Çoban²,

Suzer³

et al. 2010

Turk. J. Fish. Aquat. Sci.

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The ontogenesis and formation stages of exocrine pancreas in European sea bass (D. labrax) larvae were investigated from hatching to 40 days after hatching (DAH). Histological and enzymatical techniques were used to explain the functional development of the pancreas in D. labrax with the expression of trypsinogen activity. The incipient pancreas appeared as a lamination of the dorsal wall of the digestive tract. It was observed that the primary visible indication of exocrine cell differentiation was polarization. The first zymogen granules and pancreas with exocrine polyhedral cells appeared on 6 DAH and became abundant as a compact structure located dorsal and slightly posterior to the liver. At the same time, firstly, anus and then mouth were opened, and total lengths of larvae were determined as 3.47±0.26 mm. Until larval metamorphosis, the pancreas became diffuse, spreading throughout the mesentery enclosure, the stomach, the upper intestine and the pyloric caeca. On the other hand, zymogen granules were more numerous and larger, and a greater quantity of material was carried by the ducts, indicating an increased cellular activity. The specific activity of trypsin was determined as early as after hatching (42.54±6.8 mU/mg protein -1 ) at 4.28±0.2 mm total length of larvae and increased immediately during the following days especially after exogenous feeding. The highest tryptic activity was detected on 30 DAH as 122.45±11.76 mU/mg protein -1 . It is concluded that exocrine pancreas organogenesis is the main critical step of the zymogen granules and trypsin activity is present as early as after hatching and continuously increasing with larval period of D. labrax.Keywords: Dicentrarchus labrax, exocrine pancreas, histology, trypsin, ontogenesis, growth. Kültürü Yapılan Levrek (Dicentrarchus labrax) Larvalarında Ekzokrin Pankreas Gelişimi ve Tripsin Aktivitesi ÖzetBu çalışmada açılımdan 40. güne kadar levrek (D. labrax) larvalarında ekzokrin pankreasın ontogenik gelişimi ve oluşum aşamaları incelenmiştir. Levrek larvalarında pankreasın fonksiyonel gelişimi tanımlamak için histolojik ve tripsinojen aktivitesinin incelendiği enzimatik teknikler kullanılmıştır. İlk pankreas oluşumu sindirim tüpünün dorsal bölgesinde tabakalaşmış olarak izlenmiştir. Eksokrin hücre farklılaşmasının ilk işaretinin kutuplaşma olduğu gözlenmiştir. 6. günde ilk zimojen granülleri ve ekzokrine polihedarl hücreli pankreas karaciğerin posteiorunda ve dorsal bölgesinde konumlanmış yoğun bir oluşum olarak gözlenmiştir. Aynı zamanda, ilk olarak anüs sonrasında ağız açılmış ve larvaların total boyu 3,47±0,26 mm olarak tespit edilmiştir. Larval metamorfoza kadar, pankreas farklılaşmış ve midenin mesenterik bölgesinde, bağırsağın ve pilorik çekumun üst kısmında yayılmış olarak izlenir. Öte yandan, zimojen granüllerinin sayısının ve hacminin artması ve beraberinde taşınan içerik miktarının artışı hücresel aktivitede meydana gelen artışın açık bir göstergesidir. Tripsin aktivitesi açılımın hemen ardından (42,54±6,8 mU/mg protein -1 ) larva...

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Characterization of digestive enzymes during larval development of red drum (Sciaenops ocellatus)

Cited by 113 publications

References 37 publications

Effects of sex on characteristics and expression levels of digestive enzymes in the adult guppy Poecilia reticulata

Effects of sex on characteristics and expression levels of digestive enzymes in the adult guppy Poecilia reticulata

Partial Characterization of Digestive Proteases in the Common Snook Centropomus undecimalis

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