Characterization of Dipalmitoylphosphatidylcholine Liposomes Containing a Soybean-Derived Sterylglucoside Mixture by Differential Scanning Calorimetry, Fourier Transform Infrared Spectroscopy, and Enzymatic Assay

Abstract

Previously, we reported that dipalmitoylphosphatidylcholine liposomes (DPPC-liposomes) containing soybean-derived steryl glucoside mixtures (SG) (DPPC/SG-liposomes) accumulated in the liver, especially in parenchymal cells. DPPC/SG-liposomes and a mixture of DPPC and SG (DPPC/SG mixture) were compared by means of differential scanning calorimetry, Fourier transform infrared (FT-IR), and enzymatic assays. The results suggested that the maximum molar mixing ratios of DPPC and SG in a powder was DPPC:SG = 7:2.2. Enzymatic assays indicated that the glucose group of SG projected outward from the liposomal surface and that the amount of SG on the liposomal surface was limited, the maximum mole fraction of SG in DPPC/SG-liposomes being 0.27 (DPPC: SG = 7:2.6). FT-IR spectra indicated that the glucose group of SG interacts with the phosphate group of DPPC on the surface of liposomes, since the phosphate symmetric and asymmetric stretching bands of DPPC/ SG-liposomes were shifted to lower frequencies with increasing SG. These results suggested that the glucose group of SG projecting outward from the liposomal membrane contributes to the hepatic cellular distribution of DPPC/SG-liposomes.

Liposomal Encapsulated Ethanolic Coconut Husk Extract: Antioxidant and Antibacterial Properties

“…The peaks with wavenumbers of 2925 and 2926 cm −1 , 1612 and 1613 cm −1 , 1518 and 1519 cm −1 , 1380 and 1377 cm −1 , and 822 and 827 cm −1 in both UN‐EC1 and LP60‐EC1, respectively indicated the presence of C–H stretching for alkyl, C=C stretching for aromatic, C–H bending for alkyl, and C–Cl stretching, respectively (Maobe & Nyarango, ). The wavenumbers of 2855 and 2854 in both samples indicated the presence of CH 2 (Shimizu, Maitani, Takayama, & Nagai, ). This finding suggested the presence of amines, aldehydes, alcohols, amides, aromatics, phenols, lactones and esters, anhydride and carboxylic acids, quinones, and carbohydrates in both samples.…”

Chitosan-coated liposomes encapsulating curcumin: study of lipid–polysaccharide interactions and nanovesicle behavior

“…In addition, the spectral bands at 1086 and 1224 cm À1 represent the symmetric and antisymmetric PO2À stretching vibration of phospholipids, and the band representing the antisymmetric N + \CH 3 stretching vibrations was detected at 970 cm À1 . [65][66][67] The FTIR spectrum of curcumin (Fig. 5iv) showed a sharp peak at 3508 cm À1 indicating the phenolic O-H stretching, with a broad band in the range from 3100 to 3400 cm À1 , which is due to the n(OH) group (in enol form), C-H (methyl) at 2844 cm À1 , and C-H (aryl) at 3014 cm À1 .…”

Optimization of the Preparation of Magnetic Liposomes for the Combined Use of Magnetic Hyperthermia and Photothermia in Dual Magneto-Photothermal Cancer Therapy

“…The encapsulation of CMNPs in MLs could be also confirmed from the absence of the Fe–O stretching bond at 574 cm −1 by comparing the spectra of blank liposomes against those of MLs. In both liposomal preparations, characteristic peaks at 1097 cm −1 and 1246 cm −1 could be assigned to the phosphate group (P=O), while the CH 2 symmetric and asymmetric stretching provided peaks at 2848 cm −1 and 2916 cm −1 , respectively [ 42 ].…”