Characterization of DNA polymerase and RNA associated with A-type particles from murine myeloma cells

Robertson, Donald; Baenziger, Nancy Lewis; Dobbertin, D C; Thach, Robert E.

doi:10.1128/jvi.15.2.407-415.1975

Cited by 32 publications

(31 citation statements)

References 19 publications

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“…Initial characterizations of the MAV and solid tumor A-type particles produced by MOPC-460 plasmacytomas involved analyses of the reverse transcriptase, RNA, and proteins associated with them (32,33). Although the proteins bore certain similarities to those of standard MuLV (compare panels A, B, and F of Fig.…”

Section: Resultsmentioning

confidence: 99%

“…VOL. 32,1979 on August 27, 2020 by guest http://jvi.asm.org/ Downloaded from DISTANCE MIGRATED (cm) FIG. 3.…”

Section: Resultsmentioning

confidence: 99%

“…Solutions were then incubated at 37°C for 60 VOL. 32,1979 on August 27, 2020 by guest http://jvi.asm.org/ Downloaded from min followed by addition of 5 ,ug of TPCK-trypsin and incubation for an additional 3 h.…”

Section: Materials and Metmodsmentioning

confidence: 99%

“…It was previously reported that intracisternal A-type particles isolated from murine MOPC-460 solid tumors contain an RNA-dependent DNA polymerase (reverse transcriptase), as well as RNA which is genetically related to the RNAs of murine leukemia viruses (MuLV) (32,33).…”

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confidence: 99%

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Characterization of the proteins of intracisternal type A and extracellular oncornavirus-like particles produced by MOPC-460 myeloma cells

Robertson¹,

Jhabvala²,

Godefroy-Colburn³

et al. 1979

J Virol

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The mouse plasmacytoma cell line, MOPC-460, produces both intracisternal and intracytoplasmic A-type particles when grown as a solid tumor. When these cells are grown either as an ascites tumor or in tissue culture, a third type of particle is produced extracellularly. This particle, the "myeloma-associated virus," is closely related to, and probably an alternate form of, the intracisternal A-type particle. The proteins present in these two types of particles were compared by tryptic peptide mapping. Both types of particles were found to contain essentially the same major proteins of 76,000 (p76), 68,000 to 70,000 (p68-70), and 45,000 (p45) daltons, in addition to varying amounts of smaller proteins. The relative proportions of all these proteins varied from preparation to preparation in an

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Section: Resultsmentioning

confidence: 99%

“…VOL. 32,1979 on August 27, 2020 by guest http://jvi.asm.org/ Downloaded from DISTANCE MIGRATED (cm) FIG. 3.…”

Section: Resultsmentioning

confidence: 99%

Section: Materials and Metmodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Characterization of the proteins of intracisternal type A and extracellular oncornavirus-like particles produced by MOPC-460 myeloma cells

Robertson¹,

Jhabvala²,

Godefroy-Colburn³

et al. 1979

J Virol

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“…Intracisternal A-type particles are commonly found in a variety of transformed mouse cells and occasionally in normal mouse cells (33), and they have been present in every plasmacytoma ("myeloma") so far examined (22,31). They resemble cores of immature Bor C-type viruses and contain high-molecular-weight (60 to 70S) RNA and RNA-dependent DNA polymerase (18,24, 26, [34][35][36]. Moreover, complementary DNA (cDNA) probes prepared from MOPC-460 plasmacytoma A-type particles show considerable homology (-30%) to the genome of Moloney murine leukemia virus (MuLV) (24,26), and the major protein present in these particles (p76) has recently been found to be immunologically related to p30 from AKR MuLV (25).…”

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confidence: 99%

Characterization of infectious oncornaviruses from MOPC-460 plasmacytomas: their relation to A-type particles

Ramabhadran¹,

Hartley²,

Rowe³

et al. 1979

J Virol

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MOPC-460 mouse plasmacytoma cells produce intracellular A-type particles and extracellular oncornavirus-like particles ("myeloma-associated virus," abbreviated MAV). The genomes of these two particles are closely related. During attempts to establish infections with MOPC-460 extracellular particles, we isolated ecotropic and xenotropic infectious forms of murine leukemia virus. We have investigated the relation of these isolates to A-type particles and to MAV by nucleic acid hybridization. Using complementary DNA probes prepared from the two isolates, we found that these infectious murine leukemia viruses differ from A-type particles and from MAV. Moreover, we found that MAV is the predominant extracellular component: the ecotropic and xenotropic forms of murine leukemia virus were present at only low levels (<5%) in MAV preparations. Neither the SC-1 cells infected with ectropic murine leukemia virus nor the mink cells infected with xenotropic murine leukemia virus showed any A-type particles in their cytoplasm when examined by electron microscopy. Our inability to demonstrate infection by the A-type particle-related component, MAV, suggests that these may be defective.

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The development and degeneration of Purkinje cells in pcd mutant mice

Landis

Mullen

1978

J of Comparative Neurology

201

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Purkinje cell degeneration (pcd), an autosomal recessive mutation in the mouse, causes the postnatal death of virtually all cerebellar Purkinje cells during the third and fourth postnatal week. We have compared the postnatal development of normal and pcd mutant Purkinje cells. The early deviations from normal development involve primarily the perikaryonal polysomes and endoplasmic reticulum. Many of the mutant Purkinje cells retain abnormally the basal accumulation of polysomes, a finding which permits the identification of affected animals at postnatal day 15, one week prior to the onset of behavioral abnormalities. In addition, the affected Purkinje cells possess unusual configurations of endoplasmic reticulum with associated electron-dense particles similar to but larger than ribosomes, mature and forming intracisternal A particles and nematosomes. Before the pcd Purkinje cells degenerate they appear to receive all their appropriate synaptic contacts. Some disruption, however, of parallel fiber: Purkinje spine synaptogenesis occurs at late stages of development. Some spines lack presynaptic elements, postsynaptic thickenings are present along the dendritic shafts and parallel fibers appear to make synaptic contacts directly onto the shafts. The spectrum of early morphological changes that has been observed in pcd mutant Purkinje cells is thus far unique to this cerebellar abnormality.

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Characterization of DNA polymerase and RNA associated with A-type particles from murine myeloma cells

Cited by 32 publications

References 19 publications

Characterization of the proteins of intracisternal type A and extracellular oncornavirus-like particles produced by MOPC-460 myeloma cells

Characterization of the proteins of intracisternal type A and extracellular oncornavirus-like particles produced by MOPC-460 myeloma cells

Characterization of infectious oncornaviruses from MOPC-460 plasmacytomas: their relation to A-type particles

The development and degeneration of Purkinje cells in pcd mutant mice

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