Characterization of drought tolerance of GmDREB2 soybean mutants (Glycine max (L.) Merr) by ethyl methane sulfonate induction

Savitri, Evika Sandi; Fauziah, Shaddiqah Munawaroh

doi:10.1063/1.5061853

Cited by 8 publications

(3 citation statements)

References 8 publications

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“…As reported, RWC is expected to be used for the large-scale assessment of drought-induced mortality (DIM) risk and is an accurate predictor of drought mortality risk, which has special significance [ 27 , 48 ]. Consistent with our study, the RWC of the transgenic Lotus corniculatus with Populus euphraria PeDREB2a and Kandelia candel KcERF genes was also significantly higher than that of WT plants under drought treatment [ 47 ]. These data reveal that ScDREB2B-1 could increase the RWC of transgenic plants, thus improving their water retention capacity and drought tolerance.…”

Section: Discussionsupporting

confidence: 88%

“…Plants can reduce or eliminate the damage under drought stress and enhance their drought resistance by improving plant growth, RWC, water loss rate and other morphological, physiological and gene expression adaptations [ 40 , 44 , 45 , 46 , 47 ]. Mallikarjuna et al [ 45 ] showed that the two lines of OsDREB2A transgenic rice had significant tolerance to drought stress, while the surviving WT seedlings were short and pale green under drought stress.…”

Section: Discussionmentioning

confidence: 99%

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Sugarcane ScDREB2B-1 Confers Drought Stress Tolerance in Transgenic Nicotiana benthamiana by Regulating the ABA Signal, ROS Level and Stress-Related Gene Expression

Chen

Sun

et al. 2022

IJMS

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The dehydration-responsive element-binding protein (DREB) is a subgroup member of the AP2/ERF family and actively participates in the response of plants to abiotic stress. Although DREB genes have been studied in a variety of plant species, there are few reports of DREB genes in sugarcane (Saccharum spp.). In this study, a novel full-length cDNA sequence of the ScDREB2B-1 gene was cloned from the Saccharum hybrid ROC22, whose encoding protein contained only one AP2-conserved domain and was clustered into the DREB (A-2) subgroup. The diverse promoter elements in the ScDREB2B-1 gene and the accumulated transcripts of its homologous gene (SsAP2/ERF-107) in S. spontaneum under drought stress suggest that the ScDREB2B-1 gene may play a role in drought response. In addition, reverse transcription quantitative PCR analysis showed that the expression level of the ScDREB2B-1 gene was upregulated in the root and leaf of ROC22 under polyethylene glycol, sodium chloride and abscisic acid (ABA) treatments. The yeast two-hybrid experiment demonstrated that ScDREB2B-1 had transcriptional self-activation activity. Compared with wild-type plants, the overexpression of the ScDREB2B-1 gene improved the drought tolerance of the transgenic Nicotiana benthamiana by activating the ABA pathway to enhance the expression of the ABA-responsive gene (NbNCED) and ABA content, regulate the intracellular reactive oxygen species (ROS) level (enhance the transcripts of ROS synthase-related gene NbRbohB and the activities of catalase, peroxidase and superoxide dismutase) and increase the relative water content, proline content and expression level of osmotic stress-related genes (NbERD and NbLEA). Collectively, our data indicate that ScDREB2B-1 is a stress-inducible and ABA-responsive transcription factor gene that responds to drought stress by regulating ABA signaling, ROS levels and stress-related gene expression. This study contributes to a better understanding of the biological function of ScDREB2B-1, which could serve as a foundation for future resistance breeding in sugarcane.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Sugarcane ScDREB2B-1 Confers Drought Stress Tolerance in Transgenic Nicotiana benthamiana by Regulating the ABA Signal, ROS Level and Stress-Related Gene Expression

Chen

Sun

et al. 2022

IJMS

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“…However, soybean cultivation as intercrops faces various obstacles, one of which is lack of light due to shade [3]. The effect of 50% shade intensity gives detrimental effect on plant growth [4]. The improvement of soybean production is due to two major factors, both by expanding the planting area and by increasing productivity per unit area.…”

Section: Introductionmentioning

confidence: 99%

Growth Response of Two Soybean (Glycine Max (L.) Merr) Varieties Denna 1 And Rajabasa Subjected to Radiation Mutation Technique

Harsanti,

Tarmizi,

Widiarsih

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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One of the national initiatives to lessen reliance on imports of soybeans and attain soybean self-sufficiency by 2022 is soybean production. Gamma rays are used to irradiate the Denna 1 and Rajabasa kinds of soybean seedlings. The treatments utilized were planted in metal trays and varied from 0 to 1000 Gy. The germination rate was 40% in Rajabasa at 900 and 1000 Gy, which was the lowest, and 88.35% at 0 Gy on Denna 1. Denna 1 had the highest plant height at 0 Gy (34.53 cm), while Denna 2 had the lowest at 800 Gy (1.30 cm). Denna 1 had the most leaves (6.40 cm) at 200 Gy, whereas Rajabasa had the fewest at 400 Gy (0.50). Rajabasa-2 had the highest flowering age at M3 generation (44,33 days) and Denna-1.3 had the lowest (300 Gy) (41,67 days). Rajabasa mutant line had the maximum weight of full pods at 200 Gy (55,33 g), whereas Rajabasa-1 showed the lowest weight at 300 Gy (33 g). The outcome of this dosage orientation experiment may assist in determining the ideal dose for post-irradiated plants.

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Biotechnological Approaches for Enhancing Stress Tolerance in Legumes

Chandrashekharaiah

Paul

Kushwaha

et al. 2021

Sustainable Agriculture Reviews

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Characterization of drought tolerance of GmDREB2 soybean mutants (Glycine max (L.) Merr) by ethyl methane sulfonate induction

Cited by 8 publications

References 8 publications

Sugarcane ScDREB2B-1 Confers Drought Stress Tolerance in Transgenic Nicotiana benthamiana by Regulating the ABA Signal, ROS Level and Stress-Related Gene Expression

Sugarcane ScDREB2B-1 Confers Drought Stress Tolerance in Transgenic Nicotiana benthamiana by Regulating the ABA Signal, ROS Level and Stress-Related Gene Expression

Growth Response of Two Soybean (Glycine Max (L.) Merr) Varieties Denna 1 And Rajabasa Subjected to Radiation Mutation Technique

Biotechnological Approaches for Enhancing Stress Tolerance in Legumes

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