Characterization of Dysbiotic Changes of Skin Microbiota in Contact Sports Athletes

Мартыканова, Д С; Давлетова, Н Х; Zemlenuhin, Ilya; Mugallimov, S.M.; Ahatov, Azat M.; Karamova, Nazira S.; Malanin, Sergey; Laikov, Alexander; Markelova, Maria; Siniagina, Maria; Boulygina, Eugenia A.; Grigoryeva, Tatiana V.

doi:10.1007/s12668-017-0396-0

Cited by 3 publications

(1 citation statement)

References 17 publications

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“…Significant changes in the number of bacterial hemolytic forms after training have also not been detected. The similar situation was observed in our previous study, where the predominance of bacilli in the skin microbiota of wrestlers was confirmed during the metagenomic analysis and their hemolytic properties by cultivation on the selective media [14].…”

Section: Resultssupporting

confidence: 88%

Skin Microbiota in Contact Sports Athletes and Selection of Antiseptics for Professional Hygiene

Мартыканова

Давлетова

Zemlenuhin

et al. 2019

BioMed Research International

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Background The aim of this study was to assess changes in skin microbiota of wrestlers during training sessions and to determine the sensitivity of hemolytic bacterial isolates to antiseptics. Methods The main skin bacterial isolates obtained from the skin of 15 wrestlers were identified by cultivation method, with the following MALDI Biotyper and 16S rRNA gene sequencing methods. The sensitivity of hemolytic isolates to antiseptics (Veltosept-2, Cutasept F, Chlorhexidine, Miramistin, and Hydrogen Peroxide) was evaluated by measuring the size of bacterial growth inhibition zone on agar plates. Results Opportunistic bacteria of the species Bacillus cereus, Staphylococcus aureus, S. epidermidis, and S. saprophyticus were the most commonly found species in skin microbiota of wrestlers before and after training sessions. Representatives of all these species mostly had a hemolytic activity. An alcohol-containing antiseptic Veltosept-2 showed the strongest inhibitory effect on the bacterial isolates of athletes' skin microbiota most frequently detected in this study. Conclusions The general increase in the bacterial colonization of wrestlers' skin, as well as the presence of hemolytic forms of opportunistic bacteria in cutaneous microbiota, indicates dysbiotic changes and a decrease in the protective features of the host organism. Veltosept-2 application can reduce the incidence of skin infections in contact sports athletes with the highest efficiency.

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Section: Resultssupporting

confidence: 88%

Skin Microbiota in Contact Sports Athletes and Selection of Antiseptics for Professional Hygiene

Мартыканова

Давлетова

Zemlenuhin

et al. 2019

BioMed Research International

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Green Synthesis of Poly(vinyl alcohol)‐Silver Nanoparticles Composite Using Kendu (Diospyros melanoxylon Roxb) Fruit Peel Extract and Its Antibacterial Activity

Mohan Kisku,

Mandal,

Ghosh

et al. 2023

ChemistrySelect

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Greenly synthesized silver nanoparticles (AgNPs) are gaining attention as potential antimicrobials. We report on the green method for the synthesis of poly (vinyl alcohol) (PVA) – silver nanoparticles (PVA‐AgNPs) composite 1 using Kendu (Diospyros melanoxylon Roxb) fruit peel (KFP) aqueous extract. The use of KFP extract for the synthesis of AgNPs in the composite has been investigated for the first time. UV−visible spectroscopy and transmission electron microscopy (TEM) are employed to characterize the prepared AgNPs. The FTIR and 1H NMR spectral analyses reveal the involvement of phytochemicals of the KFP extract in the composite synthesis. The composite exhibits strong antibacterial activity against both Gram‐ negative Escherichia coli and Gram‐positive Staphylococcus aureus bacterial pathogens and action increases with AgNPs content.

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Sensitivity of staphylococcus microflora of wrestlers’ skin to bacteriophages

Мартыканова¹,

Zemlenuhin²,

Reshetnik³

et al. 2019

SCIENCE AND SPORT: Current Trends

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The purpose of this study was to identify the characteristics of bacteriocenosis of wrestlers’ skin and to determine the sensitivity of staphylococcal microflora to bacteriophages. Methods and organization of the research. The experiment involved 15 athletes aged 17-21 years engaged in national wrestling and belt wrestling. Qualifications ranged from 1st adult rank to the master of sports. We used the method of microbiological seeding on yolk-salt agar (JSA) to analyze the washes from the intact skin of the medial part of forearms of wrestlers before and immediately after training. We identified the grown colonies of microorganisms using MALDI Microflex Biotyper mass-spectrometer (Bruker, Germany). In addition to the total microbial abundance, the frequency of occurrence of hemolytic forms of bacteria on wrestlers’ skin was determined before and after training. We determined the sensitivity of Staphylococcus aureus bacteria to staphylococcal bacteriophage and polyvalent pyobacteriophage by the diameter of the bacteria lysis zone. Results and discussion. The research revealed the following facts. 1) S. aureus appears more often than other staphylococci on the skin of the medial part of wrestlers’ forearms both before and after training. 2) We detected high frequency of occurrence of hemolytic forms of Staphylococcus bacteria, which indicates the dysbiosis of wrestlers’ skin. 3) It is more efficient to use a staphylococcal bacteriophage than polyvalent pyobacteriophage for the prevention and treatment of infectious diseases of wrestlers’ skin caused by S. aureus. Conclusion. Athletes of contact sports demonstrate an increased risk of skin infectious diseases, and they need effective means of protection and prevention.

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Characterization of Dysbiotic Changes of Skin Microbiota in Contact Sports Athletes

Cited by 3 publications

References 17 publications

Skin Microbiota in Contact Sports Athletes and Selection of Antiseptics for Professional Hygiene

Skin Microbiota in Contact Sports Athletes and Selection of Antiseptics for Professional Hygiene

Green Synthesis of Poly(vinyl alcohol)‐Silver Nanoparticles Composite Using Kendu (Diospyros melanoxylon Roxb) Fruit Peel Extract and Its Antibacterial Activity

Sensitivity of staphylococcus microflora of wrestlers’ skin to bacteriophages

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