Characterization of E-selectin-deficient mice: Demonstration of overlapping function of the endothelial selectins

Labow, Mark; Norton, Christine R.; Rumberger, John M.; Lombard-Gillooly, Kathleen M.; Shuster, David J.; Hubbard, Jennifer; Bertko, R J; Knaack, P A; Terry, Robert W.; Harbison, Margaret L.; Köntgen, Frank; Stewart, Colin L.; McIntyre, Kim W.; Will, Peter C.; Burns, Daniel K.; Wolitzky, Barry A.

doi:10.1016/1074-7613(94)90041-8

Cited by 361 publications

(218 citation statements)

References 52 publications

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“…High-power ( X 132) photomicrographs of sequential arthroscopic synovial knee biopsy samples from a patient with rheumatoid arthritis. Sections were immunostained with an E-selectin (A-C) or P-selectin antibody (D-F) using a diaminobenzidine substrate (brown staining) and a methyl green nuclear counterstain (blue staining trafficking in this model (45), and E-selectin antibodies reduce cytokine-induced neutrophil rolling in Pselectin-deficient mice (46), the role of E-selectin in these models is less prominent than that of P-selectin. It is clear from our study that direct extrapolations to the in vivo situation of RA in humans cannot always be made from such in vitro studies and in vivo animal studies.…”

Section: Discussionmentioning

confidence: 99%

Effects of pulse methylprednisolone on cell adhesion molecules in the synovial membrane in rheumatoid arthritis. Reduced E‐selectin and intercellular adhesion molecule 1 expression

Youssef

Triantafillou

Parker

et al. 1996

Arthritis & Rheumatism

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Objective. To investigate the effects of a 1,000-mg intravenous pulse of methylprednisolone succinate (MP) on cell adhesion molecule expression on the synovial vascular endothelium in patients with rheumatoid arthritis (RA).Methods. Sequential arthroscopic biopsy samples were taken before and 24 hours after MP administration (10 patients) and at the time of RA flare (2 patients) and after retreatment with MP (1 patient). Immunoperoxidase staining for E-selectin (CD62E), P-selectin (CD62P), intercellular adhesion molecule 1 (ICAM-1; CD54) and platelet-endothelial cell adhesion molecule (PECAM; CD31) was performed, and the staining was quantified by color video image analysis.Results. MP caused a rapid (within 24 hours) and substantial decrease in the expression of E-selectin on the synovial vascular endothelium, with a smaller reduction in ICAM-1 expression on synovial vascular endothelium and the synovial lining. There were no similar effects on synovial membrane P-selectin or PECAM expression.Conclusion. A potential mechanism by which MP

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Section: Discussionmentioning

confidence: 99%

Effects of pulse methylprednisolone on cell adhesion molecules in the synovial membrane in rheumatoid arthritis. Reduced E‐selectin and intercellular adhesion molecule 1 expression

Youssef

Triantafillou

Parker

et al. 1996

Arthritis & Rheumatism

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“…5 Studies in knockout mice indicate that L-selectin has a general role in leucocyte trafficking, 6 while Eselectin is probably important in slow rolling and arrest of leucocytes in cytokine-stimulated endothelium. 7,8 Selectin proteins may be useful biomarkers in SLE and perhaps contribute to the disease process. Small-scale studies in lupus patients indicate that levels of soluble and membrane-bound E-selectin 9,10 and soluble L-selectin (sL-selectin) may be associated with SLE and disease activity.…”

Section: Introductionmentioning

confidence: 99%

No association between E- and L-selectin genes and SLE: soluble L-selectin levels do correlate with genotype and a subset in SLE

et al. 2005

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Altered function of selectin glycoprotein adhesion molecules may modulate severity and organ-specific manifestations of autoimmune and inflammatory disease via changes in leukocyte trafficking. Serum concentrations of selectin molecules have been suggested as useful biomarkers in systemic lupus erythematosus (SLE). We identified increased levels of soluble L-selectin (sL-selectin), but not soluble E-selectin (sE-selectin) in 278 European-Caucasian lupus patients compared to 230 healthy siblings (P ¼ 0.002). sL-selectin levels were markedly elevated in patients with IgG antiphospholipid autoantibodies (P ¼ 0.002), suggesting that perhaps sL-selectin defines a subgroup of lupus with vasculopathy. sL-selectin level was also influenced by two L-selectin polymorphisms: 665C4T, F206L in the epidermal growth factor-like domain (P ¼ 0.015) and rs12938 in the 3 0 -untranslated region (P ¼ 0.06). Having shown increased sL-selectin levels in lupus patients, we used genetics to investigate whether this was a secondary phenomena or the result of an underlying genetic mechanism. The inheritance of nine single-nucleotide polymorphisms (SNP) spanning the selectin locus was tested in 523 UK simplex SLE families. No association with SLE, or related phenotypes, was evident with any single SNP, or haplotype in family-based tests of association. Selectin polymorphisms are, therefore, unlikely to be independent factors in SLE susceptibility.

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“…Mutant mice deficient in P-selectin [5][6][7] demonstrate compromised neutrophil emigration in the peritoneal cavity during Streptococcus pneumoniae or thioglycollate-induced peritonitis, but mutants deficient in E-selectin show no such defects [4,7]. ICAM-1 mutant mice have impaired peritoneal emigration of neutrophils during either streptococcal or thioglycollate-induced peritonitis [6,8].…”

Section: Introductionmentioning

confidence: 99%

“…Inhibition of E-selectin [2,3] or P-selectin [3,4] by blocking antibodies compromises the peritoneal emigration of neutrophils during peritonitis induced by intraperitoneal injections of glycogen or thioglycollate. Mutant mice deficient in P-selectin [5][6][7] demonstrate compromised neutrophil emigration in the peritoneal cavity during Streptococcus pneumoniae or thioglycollate-induced peritonitis, but mutants deficient in E-selectin show no such defects [4,7].…”

Section: Introductionmentioning

confidence: 99%

Combinatorial requirements for adhesion molecules in mediating neutrophil emigration during bacterial peritonitis in mice

Mizgerd

Quinlan

LeBlanc

et al. 1998

Journal of Leukocyte Biology

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To investigate the requirements for adhesion molecules in neutrophil emigration during peritonitis, mice received intraperitoneal injections of Streptococcus pneumoniae while the functions of multiple adhesion molecules were blocked. Emigration after 4 h was compromised by antibodies against ICAM-1 or genetic deficiency of ICAM-1. Anti-CD11a/CD18 antibodies decreased emigration in ICAM-1 mutant mice, suggesting that ICAM-1 independent emigration requires CD11/CD18 complexes. In contrast, mice mutant in ICAM-1 plus E-selectin showed no defect in emigration, suggesting that E-selectin commits neutrophils to an ICAM-1-dependent pathway during streptococcal peritonitis. However, in mutant mice lacking the three endothelial adhesion molecules E-selectin, P-selectin, and ICAM-1, emigration after 4 h was significantly compromised. Thus, P-selectin is essential to ICAM-1-and E-selectin-independent acute peritoneal inflammation. After 24 h of peritonitis, there were no differences between WT and E-selectin/P-selectin/ ICAM-1 mutant mice, demonstrating that these endothelial adhesion molecules are not essential to neutrophil emigration during later stages of peritonitis. J. Leukoc. Biol. 64: 291-297; 1998.

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Characterization of E-selectin-deficient mice: Demonstration of overlapping function of the endothelial selectins

Cited by 361 publications

References 52 publications

Effects of pulse methylprednisolone on cell adhesion molecules in the synovial membrane in rheumatoid arthritis. Reduced E‐selectin and intercellular adhesion molecule 1 expression

Effects of pulse methylprednisolone on cell adhesion molecules in the synovial membrane in rheumatoid arthritis. Reduced E‐selectin and intercellular adhesion molecule 1 expression

No association between E- and L-selectin genes and SLE: soluble L-selectin levels do correlate with genotype and a subset in SLE

Combinatorial requirements for adhesion molecules in mediating neutrophil emigration during bacterial peritonitis in mice

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