Characterization of ecdysteroids in Drosophila melanogaster by enzyme immunoassay and nano-liquid chromatography–tandem mass spectrometry

Blais, Catherine; Blasco, Thierry; Maria, Annick; Dauphin‐Villemant, Chantal; Lafont, René

doi:10.1016/j.jchromb.2010.02.018

Cited by 25 publications

(15 citation statements)

References 28 publications

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“…The ecdysteroids included E, 20E and MaA, effectively serving as positive controls. We also identified 24-methylecdysone (24-methylE) by its intact mass and comparison with the previously reported MS/MS spectrum (Blais et al, 2010). 24-MethylE and MaA were accompanied by satellite peaks of their epimers 24-epimethylE and 24-epi-MaA: their intact masses (<1.5 ppm accuracy) and MS/MS spectra were identical to 24-methylE and MaA, respectively; however, they were eluted with different retention times (Fig.…”

Section: The Composition Of the Drosophila Ecdysteroidomesupporting

confidence: 54%

“…Methods for LC-MS/MS detection of ecdysteroids have been developed (Blais et al, 2010;Hikiba et al, 2013;Li et al, 2006;Miyashita et al, 2011). However, crude larval extracts are difficult to analyze because of the abundance of other lipids.…”

Section: The Composition Of the Drosophila Ecdysteroidomementioning

confidence: 99%

“…To understand the behavior of this network and how it responds to genetic manipulations and environmental challenges, it is essential to be able to identify and quantify the entire complement of ecdysteroids, along with their storage forms and catabolites, here termed the ecdysteroidome. Several ecdysteroids common to other insects have been identified in Drosophila (Blais et al, 2010;Bownes et al, 1984) but it is unclear whether the list is exhaustive and how it depends on the developmental stage and diet (Feldlaufer et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

“…Interestingly, lab-reared Drosophila produce not only MaA and 24-epiMaA, but also 20E (Blais et al, 2010). Drosophila are not thought to dealkylate plant and fungal sterols; rather, 20E is produced from trace amounts of cholesterol present in lab food, although the evolutionary significance of this remarkable selectivity remains unknown (Blais et al, 2010;Feldlaufer et al, 1995;Redfern, 1986).…”

Section: Introductionmentioning

confidence: 99%

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The Ecdysteroidome of Drosophila: influence of diet and development

et al. 2015

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Ecdysteroids are the hormones regulating development, physiology and fertility in arthropods, which synthesize them exclusively from dietary sterols. But how dietary sterol diversity influences the ecdysteroid profile, how animals ensure the production of desired hormones and whether there are functional differences between different ecdysteroids produced in vivo remains unknown. This is because currently there is no analytical technology for unbiased, comprehensive and quantitative assessment of the full complement of endogenous ecdysteroids. We developed a new LC-MS/MS method to screen the entire chemical space of ecdysteroid-related structures and to quantify known and newly discovered hormones and their catabolites. We quantified the ecdysteroidome in Drosophila melanogaster and investigated how the ecdysteroid profile varies with diet and development. We show that Drosophila can produce four different classes of ecdysteroids, which are obligatorily derived from four types of dietary sterol precursors. Drosophila makes makisterone A from plant sterols and epi-makisterone A from ergosterol, the major yeast sterol. However, they prefer to selectively utilize scarce ergosterol precursors to make a novel hormone 24,28-dehydromakisterone A and trace cholesterol to synthesize 20-hydroxyecdysone. Interestingly, epi-makisterone A supports only larval development, whereas all other ecdysteroids allow full adult development. We suggest that evolutionary pressure against producing epi-C-24 ecdysteroids might explain selective utilization of ergosterol precursors and the puzzling preference for cholesterol.

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Section: The Composition Of the Drosophila Ecdysteroidomesupporting

confidence: 54%

Section: The Composition Of the Drosophila Ecdysteroidomementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Ecdysteroidome of Drosophila: influence of diet and development

et al. 2015

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“…If subjected to MS/MS, Girard hydrazones undergo LC-MS/MS ( 8,20 ); however, they appeared to be far less sensitive compared with RIA.…”

Section: Supplementary Abstract Ecdysone • Drosophila Melanogaster •mentioning

confidence: 97%

Girard derivatization for LC-MS/MS profiling of endogenous ecdysteroids in Drosophila

Lavrynenko

Nedielkov

Möller

et al. 2013

Journal of Lipid Research

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Green Chromatography

2012

Handbook of Green Analytical Chemistry

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Greening the university laboratories 3.2Green laboratory experiments 3.2.1Green methods for sample pretreatment 3.2.2 Green separation using liquid-liquid, solid-phase and solventiess extractions 3.2.3Green alternatives for chemical reactions 3.2.4Green spectroscopy 3.3The place of Green Analytical Chemistry in the future of our laboratories References vi Contents 4 Publishing in Green Analytical Chemistry 55 Salvador G'arrigues and Miguel de la Guardia 4.1 A bibliometric study of the literature in Green Analytical Chemistry 56 4.2 Milestones of the literature on Green Analytical Chemistry 57 4.

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Characterization of ecdysteroids in Drosophila melanogaster by enzyme immunoassay and nano-liquid chromatography–tandem mass spectrometry

Cited by 25 publications

References 28 publications

The Ecdysteroidome of Drosophila: influence of diet and development

The Ecdysteroidome of Drosophila: influence of diet and development

Girard derivatization for LC-MS/MS profiling of endogenous ecdysteroids in Drosophila

Green Chromatography

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