Characterization of Ectomycorrhizal species through molecular biology tools and morphotyping

Clasen, Bárbara; Silveira, Andressa da; Baldoni, Daiana Bortoluzzi; Montagner, Daiane Fiuza; Jacques, Rodrigo Josemar Seminoti; Antoniolli, Zaida Inês

doi:10.1590/1678-992x-2016-0419

Cited by 19 publications

(33 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A huge number of plants and fungal species are involved in ECM and their number is increasing day by day with the new species being identified and old species being excluded by further experimentation proving their non-ECM status (Fukasawa et al, 2019). The ECM fungi forms a soil-fungus-root interface in the rhizosphere around short lateral roots of the plant and a network of hyphae termed as extra-radical mycelium, helps in translocation of water and minerals specifically Nitrogen (N), Phosphorus (P) and Potassium (K) by mobilizing them from the organic substrates to the host plant in exchange for carbohydrates as a C source to gain energy (Clasen et al, 2018). ECM fungi release the extracellular enzymes that degrade the complex organic matter from the soil like proteins, cellulose, hemicellulose, lignin, and chitin thus releasing N, P that are taken up by the plants.…”

Section: Introductionmentioning

confidence: 99%

Identification and inoculation of fungal strains from Cedrus deodara rhizosphere involve in growth and alleviation of high nitrogen stress

Jamil

Ahmed

Irshad

et al. 2020

Saudi Journal of Biological Sciences

View full text Add to dashboard Cite

Cedrus deodara is economically and ethnobotanically an important forest tree and is shown to be at decline in Northern areas of Pakistan in recent years mainly due to high concentration of Nitrogen in forests. Ectomycorrhizal (ECM) association forming fungi enables the forest trees to develop optimally by absorbing water from the rhizosphere through their absorptive hyphae and by making available the nutrients by mobilization of N and P from the organic substrates. This study was conducted to identify the ECM strains from C. deodara rhizosphere and to analyse the impact of high N load on the C. deodara seedlings to establish N critical load value for coniferous forests of Pakistan. Six new fungal strains were identified from the rhizosphere of C. deodara and were registered at GenBank (NCBI) as Emmia latemarginata strain ACE1, Aspergillus terreus strain ACE2, Purpureocillium lilacinum strain ACE3, Talaromyces pinophilus strain ACE4, A. fumigatus strain ACE5 and T. pinophilus strain ACE6 with accession numbers MH145426, MH145427, MH145428, MH145429, MH145430 and MH547115. Four out of six isolated strains were inoculated with seedlings of C. deodara singly and in consortium (CN) in combination with nitrogen load of 0 (C), 25 (T1), 50 (T2), 100 kg N ha−1 yr−1 (T3). Agronomic, physiological and gene expression studies for ExpansinA4 (EXPA4) and Cystatins (Cys) were made to analyse the impact of fungal strains in relation to high N stress. This study suggests a positive impact of T1 (25 kg N ha−1 yr−1) Nitrogen load and a negative impact of T3 (100 kg N ha−1 yr−1) on growth parameters and expression patterns of EXPA4 and Cys genes. Peroxidase (POX) activity decreased in the order ACE5 > ACE2 > C > ACE3 > ACE1 > CN. However, the results of Superoxide dismutase (SOD) showed decreasing trend in the order ACE5 > C > CN > ACE1 > ACE2 > ACE3. Strain ACE3 was shown to have a positive impact on the seedlings in terms of growth, physiology and expression of genes. Present study suggests that newly identified fungal strains showing positive impact on the growth and physiology of C. deodara could be used for the propagation of this economically important plant in Pakistan after pathogenicity test.

show abstract

Section: Introductionmentioning

confidence: 99%

Identification and inoculation of fungal strains from Cedrus deodara rhizosphere involve in growth and alleviation of high nitrogen stress

Jamil

Ahmed

Irshad

et al. 2020

Saudi Journal of Biological Sciences

View full text Add to dashboard Cite

show abstract

“…et al, 2018). ECM fungi play a determinant role in the absorption of nutrients and water by plants through establishing mutualistic interactions with plant roots (Becquer et al, 2018; Clasen et al, 2018). Saprophytic fungi are mainly responsible for the decomposition of complex organic matter and conversion of nutrients (Talbot et al, 2013; Nagati et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Variations in Soil Functional Fungal Community Structure Associated With Pure and Mixed Plantations in Typical Temperate Forests of China

Zhang

Peng

et al. 2019

Front. Microbiol.

View full text Add to dashboard Cite

Forest plants are in constant contact with the soil fungal community, which plays an important role in the circulation of nutrients through forest ecosystems. The objective of this study was to evaluate the fungal diversity in soil and elucidate the ecological role of functional fungal communities in forest ecosystems using soil samples from seven different plantations in northeastern China. Our results showed that the fungal communities were dominated by the phyla Ascomycota, Basidiomycota, and Mortierellomycota, and the mixed plantation of Fraxinus mandshurica and Pinus koraiensis had a soil fungal population clearly divergent from those in the other plantations. Additionally, the mixed plantation of F. mandshurica and P. koraiensis , which was low in soil nutrients, contained a highly diverse and abundant population of ectomycorrhizal fungi, whereas saprophytic fungi were more abundant in plantations with high soil nutrients. Redundancy analysis demonstrated a strong correlation between saprophytic fungi and the level of soil nutrients, whereas ectomycorrhizal fungi were mainly distributed in soils with low nutrient. Our findings provide insights into the importance of functional fungi and the mediation of soil nutrients in mixed plantations and reveal the effect of biodiversity on temperate forests.

show abstract

“…Typically, in a terrestrial ecosystem, different mycorrhizal symbiotic links develop between plants and fungi in an extensive range (Finlay, 2008). These symbiotic associations are abundant and occur in 75 to 80% plants (Clasen et al, 2018). Mycorrhizal association plays very important role in ecosystem which improves plants growth and existence through a mutualistic relationship (Kernaghan, 2005) as can be seen in Figure 1.…”

Section: Introductionmentioning

confidence: 99%

Sensitivity of Arbuscular Mycorrhizal Fungi in Old-Growth Forests: Direct Effect on Growth and Soil Carbon Storage

Ullah¹

2019

Appl. Ecol. Env. Res.

View full text Add to dashboard Cite

Arbuscular mycorrhizal association is ubiquitous and can be found in majority of natural habitats. Arbuscular mycorrhizal fungi (AMF) play a key role in the absorption of plant nutrients, soil aggregation and fertility. The basic role of AMF in the plant growth is to increase the absorption and translocation of relatively immobile ions and elements. In the past, studies have focused primarily on the mycorrhiza development and their general role in the growth of plants. Here, in this review manuscript, we highlight the role of AMF in the growth of old forests. In many studies, it has been found that concentration of phosphorous (P) decreases with the stand age which as a result leads to phosphorous (P) deficiency. This phosphorous (P) deficiency is considered as a cause of slow growth of old forests. In this review article, we argued that apart from the low concentration of phosphorous (P), weak arbuscular mycorrhizal associations are also a cause of slow growth of old forests. Furthermore, we also highlight the ecological role of AMF in the soil carbon storage. AMF provide physical (hyphae frame) and chemical support (Glomalin) to the dispersed soil particles to create stable aggregated soil structure. The Glomalin compound which is released by AMF acts like a glue and consequently increases soil aggregation. The soil organic matters stored in soil aggregates are less exposed to decomposition. Hence, soil can be used as a large sink where a huge amount of carbon can be stored in the form of soil organic matters which as a result can contribute to the mitigation of global climate change.

show abstract

Characterization of Ectomycorrhizal species through molecular biology tools and morphotyping

Cited by 19 publications

References 46 publications

Identification and inoculation of fungal strains from Cedrus deodara rhizosphere involve in growth and alleviation of high nitrogen stress

Identification and inoculation of fungal strains from Cedrus deodara rhizosphere involve in growth and alleviation of high nitrogen stress

Variations in Soil Functional Fungal Community Structure Associated With Pure and Mixed Plantations in Typical Temperate Forests of China

Sensitivity of Arbuscular Mycorrhizal Fungi in Old-Growth Forests: Direct Effect on Growth and Soil Carbon Storage

Contact Info

Product

Resources

About