Characterization of egg shell powder and its potential role in biomineralisation intended for vital pulp therapy

Shetty, Ashmitha Kishan; Bharadwaj, Saloni; Deveswaran, R.; Suvidha, B.; Shalini, M.; Bhumika, S. D.; Supriya, Tatikonda

doi:10.1063/5.0022512

Cited by 1 publication

(1 citation statement)

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“…In addition, the presence of trace elements (e.g. Si, Mg, St, Al, F, B, Cu) within the crystals of the NES also plays a role in increasing its solubility (Shetty et al, 2020). As copper ions were detected as trace elements and their amount was very limited from the beginning, this may explain why they become depleted by day 28, with a significant reduction in their amount from day 14 to day 28.…”

Section: Fibrosismentioning

confidence: 99%

Nano eggshell‐based slurry as a direct pulp‐capping material: In vitro characterization and histopathological assessment in an experimental animal model

Nabil Sulyiman,

El‐Rashidy,

El Moshy

et al. 2023

Int Endodontic J

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AimPulp vitality is essential for tooth integrity. Following pulp exposure, choosing a suitable pulp‐capping material is crucial to maintain pulp vitality. However, the reparative dentine bridge created by calcium hydroxide (Ca(OH)2) is generally porous and incomplete. The aim of the current study is to assess the in vitro and in vivo bioactivities of nano eggshell‐based slurry (NES), using NES as a direct pulp‐capping material, compared with Ca(OH)2 in rabbit animal model.MethodologyNano eggshell powder (NE) was characterized for particle morphology, chemical composition and ion release. In vitro bioactivity was tested by immersion in simulated body fluid (SBF) for 7 days. For histopathological evaluation, 36 adult New Zealand rabbits (72 pulp exposures) were divided into nine groups (n = 8) according to the pulp‐capping material (NES, Ca(OH)2 and no capping as negative control group) and the animals were sacrificed after 7, 14 or 28 days. The pulps of the two lower central incisors were exposed and then directly capped by Ca(OH)2 or NES or left untreated. The cavities were then sealed with glass ionomer cement. Teeth were collected for histopathological evaluation using an optical microscope. Pulp haemorrhage, inflammation, fibrosis and calcific bridge formation were assessed. Results were statistically analysed using anova and Tukey's tests.ResultsNano eggshell particles were spherical with a 20 nm diameter and were composed mainly of calcite. Statistical analysis showed that there was a significant increase in the release of all investigated ions between days 1 and 28, except for copper. NES group showed a significantly higher release of all elements as compared to Ca(OH)2. Environmental scanning electron microscope micrographs of NES incubated for 7 days in SBF showed the formation of HAp with a Ca/P ratio (1.686). For histopathological evaluation, the difference between groups was statistically significant. At day 28, 75% of the pulps of the Ca(OH)2 group showed mild calcific bridge in comparison with 100% moderate calcific bridge in the NES group. The NES group showed significantly less inflammation at days 7 and 28, and higher fibrosis at day 7 compared with Ca(OH)2.ConclusionsNano eggshell‐based slurry represents a promising novel direct pulp‐capping material with favourable pulp tissue response.

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Section: Fibrosismentioning

confidence: 99%