Characterization of Electrochemical Transducers for Biosensor Applications

Arris, Farrah Aida; Benoudjit, Abdel Mohsen; Sanober, Fahmi; Salim, Wan Wardatul Amani Wan

doi:10.1007/978-981-13-2257-0_11

Cited by 8 publications

(7 citation statements)

References 28 publications

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“…Electrochemical transducing is based on converting a chemical reaction into an electrical signal and it strictly depends on redox reaction kinetics between all electroactive components present in the reaction. This includes detected molecules, transducer material, and environmental parameters [316]. The optical photometric transducer, instead of the chemical reaction, converts radiant power generated from the Lambertian source as fluorescence, chemiluminescence, or bioluminescence into measurable signal [317].…”

Section: Chemical and Physical Aspects Of Sensingmentioning

confidence: 99%

Applications of reticular diversity in metal–organic frameworks: An ever-evolving state of the art

Ejsmont

Andreo

Lanza

et al. 2021

Coordination Chemistry Reviews

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Section: Chemical and Physical Aspects Of Sensingmentioning

confidence: 99%

Applications of reticular diversity in metal–organic frameworks: An ever-evolving state of the art

Ejsmont

Andreo

Lanza

et al. 2021

Coordination Chemistry Reviews

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“…The current trend of miniaturization of immunosensors using micro and nanotechnology promises to increase the sensitivity and multiplexing for the analysis of proteins and other biomarkers, while providing cost-effective alternatives to established assays [139]. Nanostructured electrodes can provide limits of detection at sub-nanomolar concentrations by the use of redox labels with the advantage of using simple portable instrumentation [140][141][142]. Nanoparticles can be used to increase the original concentration of the analytes using magnetic nanoparticles [143], as well as to increase the transduced signal by optical (plasmonic or quantum dots) [144][145][146] or electrochemical means (by redox reactions or by changing the conductivity) [147].…”

Section: Miniaturized Sensorsmentioning

confidence: 99%

Analytical techniques for multiplex analysis of protein biomarkers

Gool

Corrales

Čolović

et al. 2020

Expert Review of Proteomics

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Introduction: The importance of biomarkers for pharmaceutical drug development and clinical diagnostics is more significant than ever in the current shift toward personalized medicine. Biomarkers have taken a central position either as companion markers to support drug development and patient selection, or as indicators aiming to detect the earliest perturbations indicative of disease, minimizing therapeutic intervention or even enabling disease reversal. Protein biomarkers are of particular interest given their central role in biochemical pathways. Hence, capabilities to analyze multiple protein biomarkers in one assay are highly interesting for biomedical research. Areas covered: We here review multiple methods that are suitable for robust, high throughput, standardized, and affordable analysis of protein biomarkers in a multiplex format. We describe innovative developments in immunoassays, the vanguard of methods in clinical laboratories, and mass spectrometry, increasingly implemented for protein biomarker analysis. Moreover, emerging techniques are discussed with potentially improved protein capture, separation, and detection that will further boost multiplex analyses. Expert commentary: The development of clinically applied multiplex protein biomarker assays is essential as multi-protein signatures provide more comprehensive information about biological systems than single biomarkers, leading to improved insights in mechanisms of disease, diagnostics, and the effect of personalized medicine.

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“…The transducer then is used to convert the interaction of the biomolecule/bio-recognizer (like a CRP/RNA aptamer) into a measurable signal [ 10 , 11 ]. Among the various transducers, the electrochemical transducers have several advantages in detecting the aptamer–biomarkers interaction such as their sensitivity, portability, accuracy, and rapid response time [ 12 , 13 ]. Jarczewska and her co-authors have presented an electrochemical RNA-based aptasensor to measure CRP concentration by self-assembling a thiolated RNA aptamer on the surface of a bare gold electrode [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Highly Sensitive RNA-Based Electrochemical Aptasensor for the Determination of C-Reactive Protein Using Carbon Nanofiber-Chitosan Modified Screen-Printed Electrode

Tabrizi

Acedo

2022

Nanomaterials

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C-reactive protein (CRP) is one of the biomarkers related to coronavirus disease 2019 (COVID-19). Therefore, it is crucial to develop a highly sensitive, selective, and cost-effective biosensor for the determination of CRP. In this study, we designed an electrochemical aptasensor. For this purpose, the surface of a carbon screen-printed electrode was first modified with a carbon nanofiber-chitosan (CNFs-CHIT) nanocomposite. After that, the amino-terminal RNA aptamer probes were linked to the amino groups of CHIT via glutaraldehyde as the cross-linker. Finally, methylene blue (MB) as a redox probe was self-assembled on the surface of the aptasensor. The obtained results indicated that the CNFs-CHIT nanocomposite increased the surface coverage of the aptamer up to 5.9 times. The square-wave voltammetry was used for the measurement of CRP concentration in the linear range of 1.0–150.0 pM. The obtained results indicated that the signal had a logarithmic relationship with the concentration of CRP. The limit of detection (LOD) was obtained to be 0.37 pM. The dissociation constant (Kd) that demonstrates the affinity of the aptamer probe to its target was found to be 0.93 pM. The analytical performances of the proposed RNA aptasensor were better than the previously reported aptasensors for CRP. The proposed aptasensor was also applied for the determination of CRP in the human plasma samples. The obtained results indicated that there were no statistically significant differences between the responses of the proposed RNA aptasensor and an enzyme-linked immunosorbent assay kit (ELISA). The analytical performances of the proposed RNA aptasensor described in this paper are better than previously reported aptasensors for CRP determination.

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Characterization of Electrochemical Transducers for Biosensor Applications

Cited by 8 publications

References 28 publications

Applications of reticular diversity in metal–organic frameworks: An ever-evolving state of the art

Applications of reticular diversity in metal–organic frameworks: An ever-evolving state of the art

Analytical techniques for multiplex analysis of protein biomarkers

Highly Sensitive RNA-Based Electrochemical Aptasensor for the Determination of C-Reactive Protein Using Carbon Nanofiber-Chitosan Modified Screen-Printed Electrode

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