Characterization of electrospun fibers and electrospray vancomycin-containing beads through the interstitial or lamellar separation of bead composite fiber membranes to evaluate their biomedical application in vitro

Liu, Shih-Ming; Chen, Wen Cheng; Huang, Ssu-Meng; Chen, Jian-Chih; Lin, Chih-Lung

doi:10.1177/15280837221139326

Cited by 5 publications

(4 citation statements)

References 44 publications

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“…As a result, the shortening in the compression direction was greater than the expansion in the tension direction. However, the stretch deformation was less pronounced than the deformation perpendicular to the stretch axis due to the crosslink rebound effect [ 31 ]. In the presence of the crosslinking agent CA, the PVA membranes can undergo intermolecular and intramolecular crosslinking during the heating process, and the number of crosslinking points between PVA molecules increases, thereby making the membranes tougher.…”

Section: Resultsmentioning

confidence: 99%

“…According to the ASTM D882 specification for tensile tests, a PVA fibrous membrane of uniform thickness was selected. The anisotropically electrospun membrane was cut into dumbbell-shaped specimens with a mean thickness of 0.04 ± 0.01 μm [ 31 , 32 ]. The mechanical properties of the post-crosslinked PVA membranes with different concentrations of CA were tested on a universal testing machine (HT-2402, Hung Ta, Taichung, Taiwan) at an elongation rate of 5 mm/min.…”

Section: Methodsmentioning

confidence: 99%

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Effect of Citric Acid on Swelling Resistance and Physicochemical Properties of Post-Crosslinked Electrospun Polyvinyl Alcohol Fibrous Membrane

et al. 2023

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A series of electrospun polyvinyl alcohol (PVA) fiber membranes were crosslinked with citric acid (CA) at concentrations of 10, 20, and 30 wt.% (designated as CA10, CA20, and CA30). The effects of CA on the chemical structure, mechanical strength, swelling resistance, and cytotoxicity of the crosslinked PVA fibrous membranes were investigated. Infrared spectroscopy indicated the enhanced esterification of carboxyl and hydroxyl groups between CA and PVA. The modulus and strength of the electrospun PVA membrane increased due to the crosslinking between CA and PVA. The crosslinking of the PVA fiber matrix with CA increased the PVA binding point, thereby increasing the swelling resistance and modulus; however, the concentration of CA used was limited. Results showed that the water absorption of the PVA membranes decreased from 6.58 ± 0.04 g/g for CA10 to 3.56 ± 3.33 g/g for CA20 and 2.85 ± 0.40 g/g for CA30 with increasing CA. The water absorption remained unchanged after the membrane was soaked for a period of time, so no significant difference was found in the water absorption capacity of the same group after immersion from 1 h to 3 d. The tensile strength increased from 20.52 MPa of CA10 to 22.09 MPa of CA20. With an increased amount of CA used for crosslinking, the tensile strength and modulus of CA30 decreased to 11.48 and 13.94 MPa, respectively. Our study also showed that CA was not toxic to L929 cell viability when used for fiber crosslinking at less than 20 wt.% PVA, meaning it may be a good candidate as a support layer for guided tissue engineering.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Effect of Citric Acid on Swelling Resistance and Physicochemical Properties of Post-Crosslinked Electrospun Polyvinyl Alcohol Fibrous Membrane

et al. 2023

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“…Hydrogel membrane produced through electrospinning technology as a nanofiber scaffold naturally has the advantages of high specific surface area, good tension, flexible and thin film, and high porosity [ 6 , 7 , 8 ]. In addition to simulating the structure of the extracellular matrix [ 9 ], the aligned fibers of electrospun hydrogel membranes can serve as biological scaffolds to guide cell attachment and proliferation, thus performing better than fibrous membranes with randomly arranged fibers [ 10 , 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Hybrid-Aligned Fibers of Electrospun Gelatin with Antibiotic and Polycaprolactone Composite Membranes as an In Vitro Drug Delivery System to Assess the Potential Repair Capacity of Damaged Cornea

Shao,

Huang,

Liu

et al. 2024

Polymers

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The cornea lacks the ability to repair itself and must rely on transplantation to repair damaged tissue. Therefore, creating alternative therapies using dressing membranes based on tissue engineering concepts to repair corneal damage before failure has become a major research goal. Themost outstanding features that are important in reconstructing a damaged cornea are the mechanical strength and transparency of the membrane, which are the most important standard considerations. In addition, preventing infection is an important issue, especially in corneal endothelial healing processes. The purpose of this study was to produce aligned fibers via electrospinning technology using gelatin (Gel) composite polycaprolactone (PCL) as an optimal transport and antibiotic release membrane. The aim of the composite membrane is to achieve good tenacity, transparency, antibacterial properties, and in vitro biocompatibility. Results showed that the Gel and PCL composite membranes with the same electrospinning flow rate had the best transparency. The Gel impregnated with gentamicin antibiotic in composite membranes subsequently exhibited transparency and enhanced mechanical properties provided by PCL and could sustainably release the antibiotic for 48 h, achieving good antibacterial effects without causing cytotoxicity. This newly developed membrane has the advantage of preventing epidermal tissue infection during clinical operations and is expected to be used in the reconstruction of damaged cornea in the future.

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“…The use of drug carriers can overcome some problems related to the development and application of new drugs, but their use still has limitations, such as fast drug release, differences in solubility and biodistribution, etc., which will affect the interaction between the drug and the treatment site [ 5 , 6 , 7 ]. To optimize drug efficiency and reduce its side effects, many studies have prepared drug carriers by using natural or synthetic polymers through different techniques, such as emulsification, solvent evaporation, ionic gelation, self-assembly, nanoprecipitation, and supercritical fluid technology [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Characterizations of Post-Crosslinked Gelatin-Based Microspheres Modified by Phosphatidylcholine or Diammonium Phosphate as Antibiotic Delivery Vehicles

Chang

Chen

et al. 2023

Polymers

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Hydrogel-based microspheres prepared by emulsification have been widely used as drug carriers, but biocompatibility remains a challenging issue. In this study, gelatin was used as the water phase, paraffin oil was used as the oil phase, and Span 80 was used as the surfactant. Microspheres were prepared using a water-in-oil (W/O) emulsification. Diammonium phosphate (DAP) or phosphatidylcholine (PC) were further used to improve the biocompatibility of post-crosslinked gelatin microspheres. The biocompatibility of DAP-modified microspheres (0.5–10 wt.%) was better than that of PC (5 wt.%). The microspheres soaked in phosphate-buffered saline (PBS) lasted up to 26 days before fully degrading. Based on microscopic observation, the microspheres were all spherical and hollow inside. The particle size distribution ranged from 19 μm to 22 μm in diameter. The drug release analysis showed that the antibiotic gentamicin loaded on the microspheres was released in a large amount within 2 h of soaking in PBS. It was stabilized until the amount of microspheres integrated was significantly reduced after soaking for 16 days and then released again to form a two-stage drug release curve. In vitro experiments showed that DAP-modified microspheres at concentrations less than 5 wt.% had no cytotoxicity. Antibiotic-impregnated and DAP-modified microspheres had good antibacterial effects against Staphylococcus aureus and Escherichia coli, but these drug-impregnated groups hinder the biocompatibility of hydrogel microspheres. The developed drug carrier can be combined with other biomaterial matrices to form a composite for delivering drugs directly to the affected area in the future to achieve local therapeutic effects and improve the bioavailability of drugs.

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Characterization of electrospun fibers and electrospray vancomycin-containing beads through the interstitial or lamellar separation of bead composite fiber membranes to evaluate their biomedical application in vitro

Cited by 5 publications

References 44 publications

Effect of Citric Acid on Swelling Resistance and Physicochemical Properties of Post-Crosslinked Electrospun Polyvinyl Alcohol Fibrous Membrane

Effect of Citric Acid on Swelling Resistance and Physicochemical Properties of Post-Crosslinked Electrospun Polyvinyl Alcohol Fibrous Membrane

Hybrid-Aligned Fibers of Electrospun Gelatin with Antibiotic and Polycaprolactone Composite Membranes as an In Vitro Drug Delivery System to Assess the Potential Repair Capacity of Damaged Cornea

In Vitro Characterizations of Post-Crosslinked Gelatin-Based Microspheres Modified by Phosphatidylcholine or Diammonium Phosphate as Antibiotic Delivery Vehicles

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