Characterization of endoglucanase rich Trichoderma reesei cellulase mixtures and their effect on alkaline solubility of dissolving pulp

Kamppuri, Taina; Vehviläinen, Marianna; Backfolk, Kaj; Heiskanen, Isto

doi:10.1007/s10570-016-1055-2

Cited by 7 publications

(8 citation statements)

References 43 publications

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“…A recent study showed that cellulase treatments of pulps significantly increased their alkali solubility. 13 al. 16 and modified to make it suitable for native wood.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

Cellulose Elementary Fibrils Assemble into Helical Bundles in S₁ Layer of Spruce Tracheid Wall

et al. 2017

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ABSTRACT. The ultrastructural organization of cellulose elementary fibrils (EF) in wood cell wall is considered to be the prime factor regulating the material characteristics of wood in micro to macro levels and the conversion of delignified wood fibers into various products. Specifically, the complex assembly of EFs in wood cell wall limits its swellability, solubility and reactivity e.g., in dissolution of cellulose for regeneration of textile fibers, fibril separation for the manufacture of nanocellulose, and enzymatic hydrolysis of cellulose into sugars for their subsequent fermentation to various products, like ethanol for future fossil fuels replacement. Here cryo-transmission electron tomography was applied on ultrathin wood sections to reveal the EF assembly in the native cell wall. The resolution of these tomograms was then further enhanced by computational means. Direct visualization of EFs shows that they are both curved and bundled. Remarkably, EFs are observed to be often assembled into helical bundles similar to steel wire rope, a structural feature that must have a significant impact on the swelling, accessibility and solubility of woody biomass for its conversion into the aforementioned value added products.

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“…A recent study showed that cellulase treatments of pulps significantly increased their alkali solubility. 13 al. 16 and modified to make it suitable for native wood.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

Cellulose Elementary Fibrils Assemble into Helical Bundles in S₁ Layer of Spruce Tracheid Wall

et al. 2017

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“…Light and fluorescence based microscopic direct visual evidence of cellulase action on the plant cells from pretreated biomass are rare. The Primafast 200 is commercial cellulase with whole cellulase spectrum which have been explored as core enzymes for raw agrowaste biomass saccharification along with crude accessory enzymes 22–24 .…”

Section: Introductionmentioning

confidence: 99%

Valorization of sugarcane bagasse by chemical pretreatment and enzyme mediated deconstruction

Thite

Nerurkar

2019

Sci Rep

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After chemical pretreatment, improved amenability of agrowaste biomass for enzymatic saccharification needs an understanding of the effect exerted by pretreatments on biomass for enzymatic deconstruction. In present studies, NaOH, NH4OH and H2SO4 pretreatments effectively changed visible morphology imparting distinct fibrous appearance to sugarcane bagasse (SCB). Filtrate analysis after NaOH, NH4OH and H2SO4 pretreatments yielded release of soluble reducing sugars (SRS) in range of ~0.17–0.44%, ~0.38–0.75% and ~2.9–8.4% respectively. Gravimetric analysis of pretreated SCB (PSCB) biomass also revealed dry weight loss in range of ~25.8–44.8%, ~11.1–16.0% and ~28.3–38.0% by the three pretreatments in the same order. Release of soluble components other than SRS, majorly reported to be soluble lignins, were observed highest for NaOH followed by H2SO4 and NH4OH pretreatments. Decrease or absence of peaks attributed to lignin and loosened fibrous appearance of biomass during FTIR and SEM studies respectively further corroborated with our observations of lignin removal. Application of commercial cellulase increased raw SCB saccharification from 1.93% to 38.84%, 25.56% and 9.61% after NaOH, H2SO4 and NH4OH pretreatments. Structural changes brought by cell wall degrading enzymes were first time shown visually confirming the cell wall disintegration under brightfield, darkfield and fluorescence microscopy. The microscopic evidence and saccharification results proved that the chemical treatment valorized the SCB by making it amenable for enzymatic saccharification.

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“…For instance, the beatability and viscosity of chemical pulp was affected differently by different endoglucanases from Trichoderma reesei . Endoglucanase treatments have been reported to improve the alkaline solubility of dissolving pulp by carefully controlled hydrolysis of the cellulose chains …”

Section: Introductionmentioning

confidence: 99%

“…10 Endoglucanase treatments have been reported to improve the alkaline solubility of dissolving pulp by carefully controlled hydrolysis of the cellulose chains. 11 Xylanases (EC 3.2.1.8) are hydrolytic enzymes that catalyse the hydrolysis of -1,4-glycosidic linkage connecting the anhydroxylose units in xylan. In fibre treatment xylanases are not only involved in xylan removal, but also assist in fibre modification.…”

Section: Introductionmentioning

confidence: 99%

Screening of glycoside hydrolases and ionic liquids for fibre modification

Rahikainen

Anbarasan

Wahlström

et al. 2017

J of Chemical Tech & Biotech

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BACKGROUND This study elaborates the possibility to apply combined ionic liquid (IL) and enzyme treatments for pulp fibre modification. The approach involves swelling of fibre surfaces with IL followed by enzymatic modification of the disrupted fibre surface using carbohydrate active enzymes. RESULTS The capacity of seven cellulose‐dissolving or cellulose‐swelling ionic liquids to swell pulp fibres was compared. In addition, thirteen cellulases and five xylanases were screened for their IL tolerance, which determines their applicability in combined or sequential IL–enzyme treatments of fibres. Among the studied ionic liquids, 1‐ethyl‐3‐methylimidazolium dimethylphosphate ([EMIM]DMP) and 1,3‐dimethylimidazolium dimethylphosphate ([DMIM]DMP) had the strongest effect on fibre swelling. These solvents were also found to be the least inactivating for the studied enzymes. CONCLUSION Enzyme compatibility and cellulose‐dissolving capability are not two conflicting properties of an ionic liquid. © 2017 Society of Chemical Industry

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Characterization of endoglucanase rich Trichoderma reesei cellulase mixtures and their effect on alkaline solubility of dissolving pulp

Cited by 7 publications

References 43 publications

Cellulose Elementary Fibrils Assemble into Helical Bundles in S₁ Layer of Spruce Tracheid Wall

Cellulose Elementary Fibrils Assemble into Helical Bundles in S₁ Layer of Spruce Tracheid Wall

Valorization of sugarcane bagasse by chemical pretreatment and enzyme mediated deconstruction

Screening of glycoside hydrolases and ionic liquids for fibre modification

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Characterization of endoglucanase rich Trichoderma reesei cellulase mixtures and their effect on alkaline solubility of dissolving pulp

Cited by 7 publications

References 43 publications

Cellulose Elementary Fibrils Assemble into Helical Bundles in S1 Layer of Spruce Tracheid Wall

Cellulose Elementary Fibrils Assemble into Helical Bundles in S1 Layer of Spruce Tracheid Wall

Valorization of sugarcane bagasse by chemical pretreatment and enzyme mediated deconstruction

Screening of glycoside hydrolases and ionic liquids for fibre modification

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Product

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Cellulose Elementary Fibrils Assemble into Helical Bundles in S₁ Layer of Spruce Tracheid Wall

Cellulose Elementary Fibrils Assemble into Helical Bundles in S₁ Layer of Spruce Tracheid Wall