Characterization of Enteropathogenic Escherichia coli Associated with Diarrhea Among Iranian Infants

Mohammadzadeh, Mohammad; Dabiri, Hossein; Fallah, Fatemeh

doi:10.5812/pedinfect.36920

Cited by 3 publications

(2 citation statements)

References 23 publications

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“…PCR of the uldA gene was performed to confirm E. coli at the genus level. 10 PCR was performed on the pCVD432 gene for EAEC, 11 eae gene for EPEC, 12 elt, est genes for ETEC, 13 ial gene for EIEC, 4 and stx gene for EHEC. 12 The ESBL genes (bla TEM , bla CTX-M-1, 2, 8, 14, 15 , and bla SHV ), AmpC genes (bla CMY-2 , bla CIT , bla ACC , bla FOX , bla MOX and bla DHA ) and sul1,2 for sulfonamide resistance were identified by PCR as well.…”

Section: Genotypic Identificationmentioning

confidence: 99%

Multi-Drug-Resistant Diarrheagenic Escherichia coli Pathotypes in Pediatric Patients with Gastroenteritis from Central Iran

Abbasi¹,

Mondanizadeh²,

Belkum³

et al. 2020

IDR

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Background: Diarrheagenic Escherichia coli (DEC) is a significant cause of gastroenteritis and a major public health problem. This study investigates the prevalence and the antibiotic resistance patterns of DEC that were isolated from infectious diarrhea samples of pediatric patients from central Iran. Patients and Methods: Pediatric diarrhea samples were collected from 230 pediatric patients visiting the hospital. E. coli pathotypes were diagnosed by using conventional culture methods and PCR. Antibiotic resistance profiles, the frequency of multi-drug resistance (MDR), and the phenotypic and genotypic characteristics of extended spectrum-βlactamase (ESBL), AmpC and integron-associated genes were analyzed. Results: Of the 230 samples of infectious diarrhea, 91 (39.5%) produced E. coli isolates. Of these, 32 cases (35.1%) were identified as DEC by culture and PCR. The frequency of the E. coli pathotypes obtained was as follows: EAEC 11/32 (34.3%), EPEC 9/32 (28.1%), ETEC 6/32 (18.7%), EIEC 3/32 (9.3%), and EHEC 3/32 (9.3%). The antibiotic resistance rates were greater for nalidixic acid (30/32; 93.7%), ampicillin (29/32; 90.6%), and tetracycline (25/32; 78.1%) than for any of the other tested antibiotics. High levels of MDR (25/ 32; 78.1%) and the presence of ESBL (18/32; 56.2%) and AmpC (9/32; 28.1%) were observed in the DEC isolates. The isolates showed a higher frequency of the ESBL genes [bla TEM (18/18; 100%), bla CTX-M15 (17/18; 94.4%)], and AmpC [bla CIT (4/9; 44.4%) and bla DHA (4/9; 44.4%)] than of the other ESBL and AmpC genes. Conclusion: Compared to the previous study, DEC appeared to be the second-most abundant agent of diarrhea in pediatric patients after Campylobacter jejuni, with frequent MDR and ESBL presence.

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Section: Genotypic Identificationmentioning

confidence: 99%

Multi-Drug-Resistant Diarrheagenic Escherichia coli Pathotypes in Pediatric Patients with Gastroenteritis from Central Iran

Abbasi¹,

Mondanizadeh²,

Belkum³

et al. 2020

IDR

View full text Add to dashboard Cite

show abstract

“…In another study on risk factors of infantile reflux expressed in subgroup analysis by CKD subtype, low birth weight and maternal pregestational DM were considerable along with an increased risk of renal dysplasia/aplasia while low birth weight, maternal gestational DM, and maternal overweight/obesity were significantly associated with obstructive uropathy (22). Fallah et al in their study found that Enteropathogenic Escherichia coli was a major cause of diarrhea in Iranian children (23). However, patients with congenital disorders were not included in the current study.…”

Section: Discussionmentioning

confidence: 91%

Detection of Extended - Spectrum Beta - Lactamases in Escherichia coli Isolates and it’s Correlation with Vesicoureteral Reflux Nephropathy

Yousefichaijan

Rezagholizamenjany

Safi

et al. 2018

Arch Pediatr Infect Dis

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The first evaluation relationship of integron genes and the multidrug-resistance in class A ESBLs genes in enteropathogenic Escherichia coli strains isolated from children with diarrhea in Southwestern Iran

Jomehzadeh¹,

Ahmadi²,

Javaherizadeh

et al. 2020

Mol Biol Rep

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Characterization of Enteropathogenic Escherichia coli Associated with Diarrhea Among Iranian Infants

Cited by 3 publications

References 23 publications

<p>Multi-Drug-Resistant Diarrheagenic <em>Escherichia coli</em> Pathotypes in Pediatric Patients with Gastroenteritis from Central Iran</p>

<p>Multi-Drug-Resistant Diarrheagenic <em>Escherichia coli</em> Pathotypes in Pediatric Patients with Gastroenteritis from Central Iran</p>

Detection of Extended - Spectrum Beta - Lactamases in Escherichia coli Isolates and it’s Correlation with Vesicoureteral Reflux Nephropathy

The first evaluation relationship of integron genes and the multidrug-resistance in class A ESBLs genes in enteropathogenic Escherichia coli strains isolated from children with diarrhea in Southwestern Iran

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