Characterization of entomopathogenic fungi from vineyards in Argentina with potential as biological control agents against the European grapevine moth Lobesia botrana

Plantey, Rodrigo Javier López; Papura, Daciana; Couture, Carole; Thiéry, Denis; Pizzuolo, Pablo Humberto; Bertoldi, María Victoria; Lucero, Gabriela

doi:10.1007/s10526-019-09955-z

Cited by 16 publications

(12 citation statements)

References 38 publications

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“…In addition to the semi and selective media, several other artificial isolation methods have been described, including those that are commonly used for fungi isolation from soils. The most common examples are the insect bait methods involving the use of larvae of the greater wax moth, Galleria mellonella Linnaeus (Lepidoptera: Pyralidae), instar larvae of European grapevine moth, Lobesia botrana Denis and Schiffermüller (Lepidoptera: Tortricidae), and mealworm larvae, Tenebrio molitor Linnaeus (Coleoptera: Tenebrionidae) [ 65 , 66 , 67 , 68 , 69 , 70 ]. Using the traditional insect bait methods, several researchers have evaluated different bait insects from different taxa.…”

Section: The Constraints To Entomopathogenic Endophytic Fungi Application For Plant Improvement Pests and Diseases Managementmentioning

confidence: 99%

General Limitations to Endophytic Entomopathogenic Fungi Use as Plant Growth Promoters, Pests and Pathogens Biocontrol Agents

Bamisile

Siddiqui

Akutse

et al. 2021

Plants

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The multiple roles of fungal entomopathogens in host plants’ growth promotion, pest and pathogen management have drawn huge attention for investigation. Endophytic species are known to influence various activities of their associated host plants, and the endophyte-colonized plants have been demonstrated to gain huge benefits from these symbiotic associations. The potential application of fungal endophytes as alternative to inorganic fertilizers for crop improvement has often been proposed. Similarly, various strains of insect pathogenic fungi have been formulated for use as mycopesticides and have been suggested as long-term replacement for the synthetic pesticides that are commonly in use. The numerous concerns about the negative effects of synthetic chemical pesticides have also driven attention towards developing eco-friendly pest management techniques. However, several factors have been underlined to be militating the successful adoption of entomopathogenic fungi and fungal endophytes as plant promoting, pests and diseases control bio-agents. The difficulties in isolation and characterization of novel strains, negative effects of geographical location, vegetation type and human disturbance on fungal entomopathogens, are among the numerous setbacks that have been documented. Although, the latest advances in biotechnology and microbial studies have provided means of overcoming many of these problems. For instance, studies have suggested measures for mitigating the negative effects of biotic and abiotic stressors on entomopathogenic fungi in inundative application on the field, or when applied in the form of fungal endophytes. In spite of these efforts, more studies are needed to be done to achieve the goal of improving the overall effectiveness and increase in the level of acceptance of entomopathogenic fungi and their products as an integral part of the integrated pest management programs, as well as potential adoption as an alternative to inorganic fertilizers and pesticides.

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Section: The Constraints To Entomopathogenic Endophytic Fungi Application For Plant Improvement Pests and Diseases Managementmentioning

confidence: 99%

General Limitations to Endophytic Entomopathogenic Fungi Use as Plant Growth Promoters, Pests and Pathogens Biocontrol Agents

Bamisile

Siddiqui

Akutse

et al. 2021

Plants

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“…Consistent with our results, previous studies have also shown the effectiveness of EPF strains in controlling immature stages of L. botrana , including larvae and pupae [ 32 ]. Similarly, recent findings [ 65 ] showed that Metarhizium and Beauveria , which were isolated from soils under vineyards in Argentina, were effective against the fifth-instar larvae of L. botrana in vitro. Additionally, several studies have shown that Metarhizium is a successful EPF genus in the biological control of some lepidopteran pests in vitro, such as Spodoptera litura , S. frugiperda , Tuta absoluta , and Plutella xylostella [ 66 – 69 ], suggesting that strains of this genus have the potential to be used as biopesticides against several lepidopteran species.…”

Section: Discussionmentioning

confidence: 57%

“…Previous studies on the effectiveness of EPF strains against L. botrana have been particularly focused on a single developmental stage of the insect’s life cycle [ 55 , 65 , 70 , 71 ]. In this study, we have shown the strong effectiveness of M. anisopliae strains against different developmental stages of L. botrana , including larvae, pupae, and adults in vitro.…”

Section: Discussionmentioning

confidence: 99%

Dual effects of entomopathogenic fungi on control of the pest Lobesia botrana and the pathogenic fungus Eutypella microtheca on grapevine

Sammaritano

Deymié

et al. 2021

Biol Res

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Background Entomopathogenic fungi (EPF) are the natural enemies of insect pests. Nevertheless, research on the use of EPF for simultaneous prevention of pest and disease agents on the same crop is limited. In this study, we explored the potential dual effects of three strains of the EPF Metarhizium anisopliae on the control of detrimental agents of Vitis vinifera L., including different developmental stages (larvae, pupae, and adult) of the insect pest Lobesia botrana and the phytopathogenic fungus Eutypella microtheca. Methods Laboratory pathogenicity trials were performed to examine the effects of the three M. anisopliae strains on the mortality rate of L. botrana. In addition, field trials were conducted to assess the biocontrol potential of one selected M. anisopliae strain on the larval stage of L. botrana. Moreover, inhibitory effects of the three EPF strains on E. microtheca growth were examined in vitro. Results All the M. anisopliae strains were highly effective, killing all stages of L. botrana as well as inhibiting the growth of E. microtheca. The in vitro mortality of larvae treated with the strains was over 75%, whereas that of treated pupae and adults was over 85%. The three EPF strains showed similar efficacy against larvae and adult stages; nevertheless, pupal mortality was observed to be strain dependent. Mortality of L. botrana larvae ranged from 64 to 91% at field conditions. Inhibition of E. microtheca growth reached 50% in comparison to the control. Conclusions Our study showed that M. anisopliae strains were highly effective in ensuring control of two different detrimental agents of V. vinifera L., providing new evidence to support the dual effects of entomopathogenic fungi.

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“…Due to the mentioned progressive EGVM expansion and all of the damage that can they can cause to vineyards by grey mold induction [10], the interest in controlling EGVM is more pressing than ever [15]. Different effective measures to manage EGVM exist based on classical biological control methods and on the use of eco-friendly biotechnical tools (Table 1).…”

Section: Introductionmentioning

confidence: 99%

Exploring the Use of Entomopathogenic Nematodes and the Natural Products Derived from Their Symbiotic Bacteria to Control the Grapevine Moth, Lobesia botrana (Lepidoptera: Tortricidae)

Vicente-Díez

Blanco-Pérez

Chelkha

et al. 2021

Insects

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The European grapevine moth (EGVM) Lobesia botrana (Lepidoptera: Tortricidae) is a relevant pest in the Palearctic region vineyards and is present in the Americas. Their management using biological control agents and environmentally friendly biotechnical tools would reduce intensive pesticide use. The entomopathogenic nematodes (EPNs) in the families Steinernematidae and Heterorhabditidae are well-known virulent agents against arthropod pests thanks to symbiotic bacteria in the genera Xenorhabdus and Photorhabdus (respectively) that produce natural products with insecticidal potential. Novel technological advances allow field applications of EPNs and those bioactive compounds as powerful bio-tools against aerial insect pests. This study aimed to determine the viability of four EPN species (Steinernema feltiae, S. carpocapsae, S. riojaense, and Heterorhabditis bacteriophora) as biological control agents against EGVM larval instars (L1, L3, and L5) and pupae. Additionally, the bioactive compounds from their four symbiotic bacteria (Xenorhabdus bovienii, X. nematophila, X. kozodoii, and Photorhabdus laumondii subsp. laumondii, respectively) were tested as unfiltered ferment (UF) and cell-free supernatant (CFS) against the EGVM larval instars L1 and L3. All of the EPN species showed the capability of killing EGVM during the larval and pupal stages, particularly S. carpocapsae (mortalities of ~50% for L1 and >75% for L3 and L5 in only two days), followed by efficacy by S. feltiae. Similarly, the bacterial bioactive compounds produced higher larval mortality at three days against L1 (>90%) than L3 (~50%), making the application of UF more virulent than the application of CFS. Our findings indicate that both steinernematid species and their symbiotic bacterial bioactive compounds could be considered for a novel agro-technological approach to control L. botrana in vineyards. Further research into co-formulation with adjuvants is required to expand their viability when implemented for aboveground grapevine application.

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Characterization of entomopathogenic fungi from vineyards in Argentina with potential as biological control agents against the European grapevine moth Lobesia botrana

Cited by 16 publications

References 38 publications

General Limitations to Endophytic Entomopathogenic Fungi Use as Plant Growth Promoters, Pests and Pathogens Biocontrol Agents

General Limitations to Endophytic Entomopathogenic Fungi Use as Plant Growth Promoters, Pests and Pathogens Biocontrol Agents

Dual effects of entomopathogenic fungi on control of the pest Lobesia botrana and the pathogenic fungus Eutypella microtheca on grapevine

Exploring the Use of Entomopathogenic Nematodes and the Natural Products Derived from Their Symbiotic Bacteria to Control the Grapevine Moth, Lobesia botrana (Lepidoptera: Tortricidae)

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