Characterization of eosinophils and detection of eotaxin in skin chamber fluid after challenge with relevant allergen in patients with mild asthma

Fernvik,; Grönneberg,; Lundahl,; Raud, Rein; Zetterström,; Hage‐Hamsten, Van; Halldén,

doi:10.1046/j.1365-2222.1999.00572.x

Cited by 9 publications

(7 citation statements)

References 36 publications

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“…Our results indicate that M.M. 's peripheral blood eosinophils were in a state of activation since there was an increased number of eosinophils and increased expression of the activity markers EG2, CD9, and CD11b ( 2) (compared to reference material from healthy adult subjects analyzed in our laboratory). Suomalainen et al ( 13) found ECP levels to increase after cow's milk provocation.…”

Section: Discussionmentioning

confidence: 58%

A case of allergy to cow's milk hydrolysate

Nilsson

Öman

Halldén

et al. 1999

Allergy

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We here report a girl, now 3 years old, who has suffered from severe food allergy since her first year of life. She was strongly allergic to cow's milk, and had high levels of IgE antibody (AB) to casein (210 kU/I), beta-lactoglobulin (43 kU/I), and alpha-lactalbumin (23 kU/l) at 12 months of age. In addition, at the same age, she showed positive (2-4+) skin prick reactions to both unboiled and boiled formulas (Profylac, Nutramigen, and Neocate), besides being positive in RAST to Nutramigen (0.6 kU/l). During the first 3 years, IgE Ab levels against casein and Nutramigen increased to 310 and 1.6 kU/l, respectively. Furthermore, at 3 years of age, she had positive RAST to 14 of 15 tested food allergens, being negative only to codfish. Assessment of eosinophil-related markers revealed high total eosinophil count, increased eosinophil activity, and a low ratio of interferon (IFN)-gamma:IL-5, indicating enhanced IL-5 production. The food allergy was correlated to poor weight gain and increasing problems with atopic allergy in the airways.

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Section: Discussionmentioning

confidence: 58%

A case of allergy to cow's milk hydrolysate

Nilsson

Öman

Halldén

et al. 1999

Allergy

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“…For example, the receptor for eotaxin and RANTES, two of the up‐regulated chemokines which we identified, is selectively expressed on human Th2 cells, 32,33 in addition to eosinophils and basophils 34,35 . While the involvement of RANTES in Th2 rather than Th1 type responses is controversial, 36 there is extensive literature on the role of eotaxin in promoting atopic, Th2 responses 37–40 and in the selective recruitment of Th2 cells to the lung in a model of allergic airway disease 41 . As a Th2 cell response is generally thought to be disadvantageous to the control of M. tuberculosis infection, this would suggest that the Mφ response could result in the recruitment of a response which does not favour bacterial elimination.…”

Section: Discussionmentioning

confidence: 92%

Changes in gene expression in macrophages infected with Mycobacterium tuberculosis: a combined transcriptomic and proteomic approach

Ragno¹,

Romanó²,

Howell³

et al. 2001

Immunology

145

101

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SUMMARYWe investigated the changes which occur in gene expression in the human macrophage cell line, THP1, at 1, 6 and 12 hr following infection with Mycobacterium tuberculosis. The analysis was carried out at the transcriptome level, using microarrays consisting of 375 human genes generally thought to be involved in immunoregulation, and at the proteomic level, using two-dimensional gel electrophoresis and mass spectrometry. The analysis of the transcriptome using microarrays revealed that many genes were up-regulated at 6 and 12 hr. Most of these genes encoded proteins involved in cell migration and homing, including the chemokines interleukin (IL)-8, osteopontin, monocyte chemotactic protein-1 (MCP-1), macrophage in¯ammatory protein-1a (MIP-1a), regulated on activation, normal, T-cell expressed and secreted (RANTES), MIP-1b, MIP-3a, myeloid progenitor inhibitory factor-1 (MPIF-1), pulmonary and activation regulated chemokine (PARC), growth regulated gene-b (GRO-b), GRO-c, MCP-2, I-309, and the T helper 2 (Th2) and eosinophil-attracting chemokine, eotaxin. Other genes involved in cell migration which were up-regulated included the matrix metalloproteinase MMP-9, vascular endothelial growth factor (VEGF) and its receptor Flk-1, the chemokine receptor CCR3, and the cell adhesion molecules vesicular cell adhesion molecule-1 (VCAM-1) and integrin a3. In addition to the chemokine response, genes encoding the proin¯ammatory cytokines IL-1b (showing a 433-fold induction), IL-2 and tumour necrosis factor-a (TNF-a), were also found to be induced at 6 and/or 12 hr. It was more dif®cult to detect changes using the proteomic approach. Nevertheless, IL-1b was again shown to be strongly up-regulated. The enzyme manganese superoxide dismutase was also found to be strongly up-regulated; this enzyme was found to be macrophage-, rather than M. tuberculosis, derived. The heat-shock protein hsp27 was found to be down-regulated following infection. We also identi®ed a mycobacterial protein, the product of the atpD gene (thought to be involved in the regulation of cytoplasmic pH) in the infected macrophage extracts.

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“…The in vivo correlation of chemokine expression and eosinophil infiltration has recently been demonstrated (41). Using the skin chamber technique in human atopic subjects, skin blisters were induced after allergen challenge with relevant allergen.…”

Section: The Role Of Chemokines In Allergic Skin Diseasesmentioning

confidence: 99%

“…The number of eosinophils was significantly increased in the allergen‐challenged chambers, and elevated levels of released ECP were found. In the skin chamber fluids, higher peptide levels of eotaxin were detected in the allergen‐challenged individuals than in controls (41). Therefore, eotaxin seems to be of relevance for the recruitment of eosinophils into the skin after allergen challenge in atopic dermatitis (Fig.…”

Section: The Role Of Chemokines In Allergic Skin Diseasesmentioning

confidence: 99%

Chemokine receptor antagonists: a novel therapeutic approach in allergic diseases

Elsner

Escher

Forssmann

2004

Allergy

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The aim of this review is to give an overview of the role of chemokines, particularly ligands of the CC chemokine receptor CCR3, in allergic diseases and to show the new concept in the treatment of allergies using chemokine receptor antagonists. Allergic diseases such as allergic asthma, allergic rhinitis and atopic dermatitis are characterized by a complex interaction of different cell types and mediators. Among this, Th2 cells, mast cells, basophils and eosinophils are found in the inflamed tissue due to the attraction of chemokines. Of all the known chemokine receptors, the chemokine receptor CCR3 seems to play the major role in allergic diseases which is supported by the detection of this receptor on the cell types mentioned above. Therefore, academic and industrial research focus on compounds to block this receptor. To date, certain chemokine receptor antagonists derived from peptides and small molecules exist to block the chemokine receptor CCR3. However, the in vivo data about these compounds and the mechanisms of receptor interaction are poorly understood, as yet. For the development of additional chemokine receptor antagonists, more details about the interaction between the ligands and their receptors are required. Therefore, additional studies will lead to the identification of novel CCR3 chemokine receptor antagonists, which can be therapeutically used in allergic asthma, allergic rhinitis, and atopic dermatitis.

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Characterization of eosinophils and detection of eotaxin in skin chamber fluid after challenge with relevant allergen in patients with mild asthma

Cited by 9 publications

References 36 publications

A case of allergy to cow's milk hydrolysate

A case of allergy to cow's milk hydrolysate

Changes in gene expression in macrophages infected with Mycobacterium tuberculosis: a combined transcriptomic and proteomic approach

Chemokine receptor antagonists: a novel therapeutic approach in allergic diseases

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