Characterization of epidermal neural crest stem cell (EPI-NCSC) grafts in the lesioned spinal cord

Sieber‐Blum, Maya; Schnell, Lisa; Grim, Miloš; Hu, Yao; Schneider, René Peter; Schwab, Martin E.

doi:10.1016/j.mcn.2006.02.003

Cited by 117 publications

(112 citation statements)

References 65 publications

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“…Initially derived from rodents, now several groups reported isolation of SKPs from human scalp, foreskin, arm, beard and chin, in subjects ranging from fetal to old age [22,23,30,31,34,[36][37][38][39]. Transplantation of SKPs to animal models of disease has raised expectations for their potential use in human cell therapy [23][24][25][26][27][28][29]. However, translation of results from animal models to a human setting has often resulted in the past in inconsistencies or plain lack of success.…”

Section: Discussionmentioning

confidence: 99%

“…These progenitor cells have been reported to reside in the dermal papillae (DP) of human hair follicles [21,22], their physiological role being unclear at the moment. This easily obtainable multipotent cell population has raised expectations because of their potential use in cell therapy of neurodegeneration [23][24][25][26][27][28][29]. However, we still lack a clear understanding of the spatiotemporal abundance and phenotype of human SKPs.…”

Section: Introductionmentioning

confidence: 99%

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Age-Dependent Depletion of Human Skin-Derived Progenitor Cells

Gago¹,

Pérez-López²,

Sanz-Jaka

et al. 2009

Stem Cells

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A major unanswered question in autologous cell therapy is the appropriate timing for cell isolation. Many of the putative target diseases arise with old age and previous evidence, mainly from animal models, suggests that the stem/progenitor cell pool decreases steadily with age. Studies with human cells have been generally hampered to date by poor sample availability. In recent years, several laboratories have reported on the existence, both in rodents and humans, of skin-derived precursor (SKP) cells with the capacity to generate neural and mesodermal progenies. This easily obtainable multipotent cell population has raised expectations for their potential use in cell therapy of neurodegeneration. However, we still lack a clear understanding of the spatiotemporal abundance and phenotype of human SKPs. Here we show an analysis of human SKP abundance and in vitro differentiation potential, by using SKPs isolated from four distinct anatomic sites (abdomen, breast, foreskin, and scalp) from 102 healthy subjects aged 8 months to 85 years. Human SKP abundance and differentiation potential decrease sharply with age, being extremely difficult to isolate, expand, and differentiate when obtained from the elderly. Our data suggest preserving human SKP cell banks early in life would be desirable for use in clinical protocols in the aging population.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Age-Dependent Depletion of Human Skin-Derived Progenitor Cells

Gago¹,

Pérez-López²,

Sanz-Jaka

et al. 2009

Stem Cells

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“…(21) These cells have the capacity to express neuronal markers if implanted into the spinal cord. (22) It is likely that these cells are related to the neural-crest-derived Merkel cells, (110) a population of cells that express two genes essential for hair cell development, Pou4f3 (111)(112)(113)(114) and Atoh1. (67,115) These cells seem to retain their gene expression profile while proliferating.…”

Section: Forming the Right Number Of Hair Cells: Complex Regulations mentioning

confidence: 99%

“…(17,18) However, only a limited set of stem cell sources have been investigated. Thus far, the easily accessible stem cells derived from hair follicles (19)(20)(21)(22) have not been explored for ear regeneration.…”

Section: Introductionmentioning

confidence: 99%

The molecular basis of neurosensory cell formation in ear development: a blueprint for hair cell and sensory neuron regeneration?

2006

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SummaryThe inner ear of mammals uses neurosensory cells derived from the embryonic ear for mechanoelectric transduction of vestibular and auditory stimuli (the hair cells) and conducts this information to the brain via sensory neurons. As with most other neurons of mammals, lost hair cells and sensory neurons are not spontaneously replaced and result instead in age-dependent progressive hearing loss. We review the molecular basis of neurosensory development in the mouse ear to provide a blueprint for possible enhancement of therapeutically useful transformation of stem cells into lost neurosensory cells. We identify several readily available adult sources of stem cells that express, like the ectoderm-derived ear, genes known to be essential for ear development. Use of these stem cells combined with molecular insights into neurosensory cell specification and proliferation regulation of the ear, might allow for neurosensory regeneration of mammalian ears in the near future.

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“…136 Importantly, similar to ASCs, intraspinal administration of NCSCs did not result in the formation of tumours or teratomas. 137 Many studies have shown that transplantation of NCSCs could induce the regeneration of connective tissues, SMCs, skeletal muscle and endothelium. In 2008, the feasibility of using human NCSCs for corpus cavernosum repair and potential differentiation of NCSCs into penile cavernous sinus ECs and SMCs was investigated.…”

Section: Neural Crest Stem Cells (Ncscs)mentioning

confidence: 99%