1996
DOI: 10.17660/actahortic.1996.411.37
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Characterization of Erwinia Amylovora Strains From Different Hosts and Geographical Areas

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Cited by 21 publications
(25 citation statements)
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“…The analysis can be used to differentiate a group of isolates from plants belonging to the subfamily Pomoideae, from the isolates originating from plants belonging to the genus Rubus. Among the latter group, two subgroups were distinguished: Rubus I and Rubus II (Kim et al 1996). In case of strains from Bulgaria, it was found that 41 out of 95 carbon sources were differentially used by the strains and numerical analysis of BIOLOG results allowed to distinguish five groups within tested strains.…”
Section: Host Range and Virulencementioning
confidence: 98%
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“…The analysis can be used to differentiate a group of isolates from plants belonging to the subfamily Pomoideae, from the isolates originating from plants belonging to the genus Rubus. Among the latter group, two subgroups were distinguished: Rubus I and Rubus II (Kim et al 1996). In case of strains from Bulgaria, it was found that 41 out of 95 carbon sources were differentially used by the strains and numerical analysis of BIOLOG results allowed to distinguish five groups within tested strains.…”
Section: Host Range and Virulencementioning
confidence: 98%
“…Of the primers complementary to the pEA29 plasmid DNA, a few (Bereswill et al 1992;Llop et al 2000;McManus and Jones 1995a) allow the amplification of 0.9 kb plasmid fragment flanked by PstI digestion sites. Several authors, however, obtained products of different length in amplification with some of these primer sets (Brown et al 1996;Kim et al 1996;Lecomte et al 1997). Restriction analysis of these products showed that in this plasmid fragment, an insertion which has the size of 30-90 bp occurs (Lecomte et al 1997).…”
Section: Analysis Of Nucleic Acidsmentioning
confidence: 99%
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“…Basándose nuevamente en la utilización de carbohidratos, Kim et al (1996) et al (1991), se demostró que sólo tres de las doce cepas ensayadas fueron capaces de producir este metabolito.…”
Section: Caracterización Fenotípicaunclassified
“…Éstos se encuentran en varias copias en el genoma procariótico, y la región que los separa está menos conservada que los genes que la flanquean. Este método se ha aplicado a E. amylovora en varias ocasiones (McManus y Jones, 1995b;Jeng et al, 1999;McGhee et al, 2002b Sabat et al, 2003).En el producto de PCR obtenido a partir del plásmido de 29 kb de E. amylovora se ha observado una ligera variación de tamaño entre cepas McManus y Jones, 1995a;Kim et al, 1996;Lecomte et al, 1997;Momol et al, 1997), debida a la presencia de una serie de SSR. Esta estructura repetitiva consta de 8 nucleótidos (GAATTACA) y aparece de 3 a 15 veces en el fragmento amplificado en la pEA29-PCR .…”
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