Characterization of estrogenic receptor agonists and evaluation of estrogenic activity in the sediments of Liaohe River protected areas

Ke, Xin; Wang, Chunyong; Zhang, Haijun; Zhang, Yun; Gui, Shaofeng

doi:10.1016/j.marpolbul.2015.09.008

Cited by 18 publications

(3 citation statements)

References 37 publications

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“…Such methods have been established and applied for endocrine disrupters. For example, in the process of estrogen-like substances causing breast cancer in mammals [90], and damage on the reproductive system of fish [91], amphibians [92] and birds [93], ER activation and cell proliferation are considered as MIE and early KE, respectively [94]. However, at present, the research on pollutant immunotoxicity is so scarce that the mechanisms of action are still unclear, and that there are significant knowledge gaps for a complete AOP network.…”

Section: Establishment Of Immune Aop For Htsmentioning

confidence: 99%

Immunotoxicity In Vitro Assays for Environmental Pollutants under Paradigm Shift in Toxicity Tests

Wang

et al. 2022

IJERPH

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With the outbreak of COVID-19, increasingly more attention has been paid to the effects of environmental factors on the immune system of organisms, because environmental pollutants may act in synergy with viruses by affecting the immunity of organisms. The immune system is a developing defense system formed by all metazoans in the course of struggling with various internal and external factors, whose damage may lead to increased susceptibility to pathogens and diseases. Due to a greater vulnerability of the immune system, immunotoxicity has the potential to be the early event of other toxic effects, and should be incorporated into environmental risk assessment. However, compared with other toxicity endpoints, e.g., genotoxicity, endocrine toxicity, or developmental toxicity, there are many challenges for the immunotoxicity test of environmental pollutants; this is due to the lack of detailed mechanisms of action and reliable assay methods. In addition, with the strong appeal for animal-free experiments, there has been a significant shift in the toxicity test paradigm, from traditional animal experiments to high-throughput in vitro assays that rely on cell lines. Therefore, there is an urgent need to build high-though put immunotoxicity test methods to screen massive environmental pollutants. This paper reviews the common methods of immunotoxicity assays, including assays for direct immunotoxicity and skin sensitization. Direct immunotoxicity mainly refers to immunosuppression, for which the assays mostly use mixed immune cells or isolated single cells from animals with obvious problems, such as high cost, complex experimental operation, strong variability and so on. Meanwhile, there have been no stable and standard cell lines targeting immune functions developed for high-throughput tests. Compared with direct immunotoxicity, skin sensitizer screening has developed relatively mature in vitro assay methods based on an adverse outcome pathway (AOP), which points out the way forward for the paradigm shift in toxicity tests. According to the experience of skin sensitizer screening, this paper proposes that we also should seek appropriate nodes and establish more complete AOPs for immunosuppression and other immune-mediated diseases. Then, effective in vitro immunotoxicity assay methods can be developed targeting key events, simultaneously coordinating the studies of the chemical immunotoxicity mechanism, and further promoting the paradigm shift in the immunotoxicity test.

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Section: Establishment Of Immune Aop For Htsmentioning

confidence: 99%

Immunotoxicity In Vitro Assays for Environmental Pollutants under Paradigm Shift in Toxicity Tests

Wang

et al. 2022

IJERPH

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“…The Liaohe River Protected Area was established in 2010, mainly to establish ecological protection planning for protected areas, to carry out ecological assessment. At present, the study of sediments in the Liaohe River Protected Area is mainly focused on heavy metals, endocrine disrupting chemicals, AhR-agonists, and organ chlorine [17][18][19][20].…”

Section: Study Area and Sample Collectionmentioning

confidence: 99%

Distribution, Source and Potential Risk Assessment of Polychlorinated Biphenyls (PCBs) in Sediments from the Liaohe River Protected Area, China

Yun

Wang

2021

Sustainability

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This study aimed to assess the occurrence, distribution, toxicity equivalency and health risks of dl-PCBs (dioxin-like PCBs) from nine sites collected in surface sediments from Liaohe River Protected Area. ∑dl-PCBs concentrations in sediments range from 79.2 to 365.1 pg/g. Sediment profiles showed that pentachlorobiphenyl is the most abundant congener among all sampling sites. The results of principal component analysis and cluster analysis indicated that PCBs were mainly derived from electronic waste and paint additives in the sediments of Liaohe River Protected Area. Toxic equivalent quantity (TEQ) values of the PCBs in the Liaohe River Protected Area sediments are at comparatively lower levels compared with the previously reported data. Hazardous ratio (HR) for human health risk assessment allied to cancer was found to be lower than the non-carcinogenic risk assessment within an acceptable range.

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“…Both are complementary and thus their integration using chemical and bioanalytical tools for the characterization of sediment contamination should provide a more comprehensive picture of ecotoxicological risks to aquatic organisms [2]. In addition to effect concentrations, bioassays may provide information on modes of action (MoA) and may thus improve the diagnosis of risks in sediment samples [3][4][5][6][7][8][9][10]. In the last years, batteries of sensitive, rapid and cost-effective in vitro bioassays have been proposed as promising tools for the toxicological profiling of environmental samples and were also successfully applied for the characterization of aquatic sediments [11][12][13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Toxicity and neurotoxicity profiling of contaminated sediments from Gulf of Bothnia (Sweden): a multi-endpoint assay with Zebrafish embryos

et al. 2019

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Background: The toxicological characterization of sediments is an essential task to monitor the quality of aquatic environments. Many hazardous pollutants may accumulate in sediments and pose a risk to the aquatic community. The present study provides an attempt to integrate a diagnostic whole mixture assessment workflow based on a slightly modified Danio rerio embryo acute toxicity test with chemical characterization. Danio rerio embryos were directly exposed to sieved sediment (≤ 63 μm) for 96 h. Sediment samples were collected from three polluted sites (Kramfors, Sundsvall and Örnsköldsvik) in the Gulf of Bothnia (Sweden) which are characterized by a long history of pulp and paper industry impact. Effect data were supported by chemical analyses of 237 organic pollutants and 30 trace elements. Results: The results show that malformations and neurotoxic compounds are the main drivers of differentiation in chemical and effects analyses, respectively. Specific spinal cord malformations and delayed hatching were observed only in sediments from Kramfors while light hyperactivity was seen only after exposure to sediments from Sundsvall. Conclusions: Our experiments demonstrate that specific chemical profiles lead to specific effect patterns in Danio rerio embryos. In fact, behavioral endpoints could help detect the exposure to neurotoxins, and the observation of body malformations seems to be a potential tool for the identification of site-specific pollutants as polychlorinated biphenyl (PCBs), brominated flame retardants (BFRs) and several pesticides. Overall, results show the suitability of Danio rerio embryos for the fast screening of sediment samples.

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Characterization of estrogenic receptor agonists and evaluation of estrogenic activity in the sediments of Liaohe River protected areas

Cited by 18 publications

References 37 publications

Immunotoxicity In Vitro Assays for Environmental Pollutants under Paradigm Shift in Toxicity Tests

Immunotoxicity In Vitro Assays for Environmental Pollutants under Paradigm Shift in Toxicity Tests

Distribution, Source and Potential Risk Assessment of Polychlorinated Biphenyls (PCBs) in Sediments from the Liaohe River Protected Area, China

Toxicity and neurotoxicity profiling of contaminated sediments from Gulf of Bothnia (Sweden): a multi-endpoint assay with Zebrafish embryos

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