Characterization of Extensively Drug-Resistant Salmonella enterica Serovar Kentucky Sequence Type 198 Isolates from Chicken Meat Products in Xuancheng, China

Jiang, Yue; Wang, Zhenyu; Li, Qiuchun; Lu, Meng-Jun; Wu, Han; Mei, Cai-Yue; Shen, Ping; Jiao, Xinan; Wang, Jing

doi:10.1128/spectrum.03219-22

Cited by 6 publications

(5 citation statements)

References 29 publications

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“…Kentucky ST198 strains. 10 , 11 Additionally, increasing dynamics were observed in several resistance genes including bla TEM-1b , aph(3’)-Ia, floR , and dfrA14 in Salmonella from China. 22 …”

Section: Discussionmentioning

confidence: 99%

“…Kentucky ST198 strains. 10 , 11 Multiple resistance genes such as bla TEM-1b , aph(3’)-Ia, aadA17, aac(3)-IId, floR, qnrS1, dfrA14, lnu (F), mph (A), and arr-2 were previously detected within the multiresistance region on the chromosomes in S . Kentucky ST198 strains.…”

Section: Discussionmentioning

confidence: 99%

“…Kentucky strains contained the bla CTX-M-55 gene, which is frequently reported in both animals and humans in China 27 and has been sporadically reported in subclade ST198.2–2 from China. 10 , 11 Mutations in gyrA and parC genes and the presence of PMQR genes (eg, qnrS1 ) have been the primary causes of fluoroquinolone resistance in Salmonella , including S . Kentucky ST198 clone.…”

Section: Discussionmentioning

confidence: 99%

“…This dataset consists of 27 strains collected from patients and food products in China from 2013 to 2017, 7 one isolate from a patient in 2022, 19 and our previously described ten strains from chicken meat in 2019. 11 To analyze their genetic relatedness, a phylogenetic tree was constructed using Parsnp (https://harvest.readthedocs.io/en/latest/content/parsnp.html), based on core genome single-nucleotide polymorphisms (cgSNPs). The genome of S. Kentucky strain AH19MCS1 (accession number CP102719.1) was used as a reference for comparison.…”

Section: Phylogenetic Analysismentioning

confidence: 99%

“…[5][6][7][8][9][10] Recently, there has been a growing prevalence of ciprofloxacin-and third-generation cephalosporin-resistant S. Kentucky ST198 strains worldwide. 6,7,[10][11][12] Inflammatory bowel diseases (IBDs) are chronic inflammatory diseases of the gastrointestinal tract characterized by a relapsing and remitting course, with Crohn's disease and ulcerative colitis being the most common clinical manifestations. 13,14 Although the etiology of IBD is not well defined, environmental, genetic, and dietary risk factors influence or contribute to the development and severity of this disease.…”

Section: Introductionmentioning

confidence: 99%

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Persistent Colonization of Ciprofloxacin-Resistant and Extended-Spectrum β-Lactamase (ESBL)-Producing Salmonella enterica Serovar Kentucky ST198 in a Patient with Inflammatory Bowel Disease

Jiang,

Yang,

Wang

et al. 2024

IDR

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Salmonella enterica serovar Kentucky ST198 has emerged as a global threat to humans. In this study, we aimed to characterize the prolonged carriage of ciprofloxacin-resistant and extended-spectrum β-lactamase (ESBL)-producing S. Kentucky ST198 in a single patient with inflammatory bowel disease (IBD). Methods: Three S. Kentucky strains were collected from a single patient with IBD on 11th January, 23rd January, and 8th February, 2022, respectively. Antimicrobial susceptibility testing, whole-genome sequencing, and phylogenetic analysis with 38 previously described Chinese S. Kentucky ST198 strains from patients and food were performed. Results: All three S. Kentucky isolates belonged to ST198. They carried identical 16 resistance genes, such as bla CTX-M-55 , tet(A), and qnrS1, and had identical mutations within gyrA (S83F and D87N) and parC (S80I). Therefore, they exhibited identical multidrugresistant profiles, including the clinically important antibiotics cephalosporins (ceftazidime and cefepime), fluoroquinolones (ciprofloxacin and levofloxacin), and third-generation tetracycline (tigecycline). Our three S. Kentucky strains were classified into the subclade ST198.2-2, and were genetically identical (2-6 SNPs) to each other. They exhibited a close genetic similarity (15-20 SNPs) to the isolate NT-h3189 from a patient and AH19MCS1 from chicken meat in China, indicating a possible epidemiological link between these S. Kentucky ST198 isolates from the patients and chicken meat. Conclusion: Long-term colonization of ciprofloxacin-resistant and ESBL-producing S. Kentucky ST198 in a single patient is a matter of concern. Due to the potential transfer of S. Kentucky ST198 from food sources to humans, ongoing surveillance of this particular clone in animals, animal-derived food products, and humans should be strengthened.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%