Characterization of Extraintestinal Escherichia coli Isolated from a Peacock (Pavo cristatus) with Colisepticemia

Barbieri, Nicolle Lima; Tejkowski, Thiago Moreira; Oliveira, Aline Luísa de; Brito, Benito Guimarães de; Horn, Fabiana

doi:10.1637/9921-090811-case.1

Cited by 12 publications

(9 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bacterial DNA was obtained from whole organisms by boiling [22]. The extracts were stored at 4°C, and the supernatants were used as templates for gene amplification.…”

Section: Methodsmentioning

confidence: 99%

“…The presence of 33 virulence-associated genes in the isolates (Table 1) was investigated using multiplex polymerase chain reactions as described [23] with a few modifications, as outlined in [22].…”

Section: Methodsmentioning

confidence: 99%

“…This method is based on the variability of the ribosomal 16S-23S intergenic spacer region (ISR), which is well-distributed among isolates and has slow rates of mutation, and hence is considered useful for measuring intra-species diversity [26], [27]. The ISR region was amplified and digested with restriction enzymes ( RsaI , HinfI or TaqI ) as previously described [22]. ARDRA restriction fragment length polymorphism profiles were analyzed by eye, and were converted into two-dimensional binary matrices according to the following criteria: 1 if a band was present, and 0 if it was absent.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Genotypes and Pathogenicity of Cellulitis Isolates Reveal Traits That Modulate APEC Virulence

Barbieri

Oliveira

Tejkowski³

et al. 2013

PLoS ONE

Self Cite

View full text Add to dashboard Cite

We characterized 144 Escherichia coli isolates from severe cellulitis lesions in broiler chickens from South Brazil. Analysis of susceptibility to 15 antimicrobials revealed frequencies of resistance of less than 30% for most antimicrobials except tetracycline (70%) and sulphonamides (60%). The genotyping of 34 virulence-associated genes revealed that all the isolates harbored virulence factors related to adhesion, iron acquisition and serum resistance, which are characteristic of the avian pathogenic E. coli (APEC) pathotype. ColV plasmid-associated genes (cvi/cva, iroN, iss, iucD, sitD, traT, tsh) were especially frequent among the isolates (from 66.6% to 89.6%). According to the Clermont method of ECOR phylogenetic typing, isolates belonged to group D (47.2%), to group A (27.8%), to group B2 (17.4%) and to group B1 (7.6%); the group B2 isolates contained the highest number of virulence-associated genes. Clonal relationship analysis using the ARDRA method revealed a similarity level of 57% or higher among isolates, but no endemic clone. The virulence of the isolates was confirmed in vivo in one-day-old chicks. Most isolates (72.9%) killed all infected chicks within 7 days, and 65 isolates (38.1%) killed most of them within 24 hours. In order to analyze differences in virulence among the APEC isolates, we created a pathogenicity score by combining the times of death with the clinical symptoms noted. By looking for significant associations between the presence of virulence-associated genes and the pathogenicity score, we found that the presence of genes for invasins ibeA and gimB and for group II capsule KpsMTII increased virulence, while the presence of pic decreased virulence. The fact that ibeA, gimB and KpsMTII are characteristic of neonatal meningitis E. coli (NMEC) suggests that genes of NMEC in APEC increase virulence of strains.

show abstract

“…Bacterial DNA was obtained from whole organisms by boiling [22]. The extracts were stored at 4°C, and the supernatants were used as templates for gene amplification.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Genotypes and Pathogenicity of Cellulitis Isolates Reveal Traits That Modulate APEC Virulence

Barbieri

Oliveira

Tejkowski³

et al. 2013

PLoS ONE

Self Cite

View full text Add to dashboard Cite

show abstract

“…A pathogenicity score (PS), which takes into account the time that a chick survives after subcutaneous inoculation and the lesions observed at necropsy, was attributed to each strain by the method of Souza et al described in Barbieri et al (2012). The PS ranges from 0 (avirulent strain) to 10 (highly virulent strain).…”

Section: Pathogenicity Scoringmentioning

confidence: 99%

Prevalence of ColV Plasmid-Linked Genes andIn VivoPathogenicity of Avian Strains ofEscherichia coli

Oliveira

Rocha

Finkler³

et al. 2015

Foodborne Pathogens and Disease

Self Cite

View full text Add to dashboard Cite

Avian pathogenic Escherichia coli (APEC) causes extraintestinal infections in birds, leading to an increase in the cost of poultry production. The ColV plasmid-linked genes iroN, ompT, hlyF, iss, and iutA have previously been suggested to be predictors of the virulence of APEC. In this research, we analyzed the frequencies of these genes in a Brazilian collection of E. coli isolated from birds with colibacillosis (APEC) and from apparently healthy birds (avian fecal [A(fecal)]), as well as from the litter of poultry houses of apparently healthy flocks (avian litter [A(litter)]). All the isolates that harbored ompT also harbored hlyF, so they were considered as one trait for statistical analysis. The relationship between in vivo virulence in 1-day-old chicks, expressed as a pathogenicity score, and the number of genes in each isolate showed that isolates with less than two of the four genes were rarely pathogenic, while most pathogenic isolates contained two or more genes. Nevertheless, about half of the nonpathogenic isolates also harbored two or more genes, in agreement with previous observations that commensal E. coli isolates from the birds' microbiota can serve as a reservoir of virulence genes. Thus, the pentaplex polymerase chain reaction can be used to indicate that a strain carrying none or only one gene would be nonpathogenic, but it cannot be used to indicate that a strain with two to four genes would be an APEC. Isolates allocated to phylogenetic group B2, which is frequently associated with extraintestinal infections, had the highest pathogenicity scores, while isolates allocated to group B1 had the lowest.

show abstract

“…About the pathogenicity of E. coli, inoculation assays using chicks have been performed in order to evaluate the in vivo expression of virulence factors of APEC (SOUZA, 2010;BARBIERI et al, 2012;BARBIERI et al, 2013). Nevertheless, there are few studies correlating the degree of pathogenicity of the microorganism to the findings of resistance or sensitivity to antimicrobial agents, especially when it comes to samples of environmental origin.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial susceptibility and pathogenicity of Escherichia coli strains of environmental origin

et al. 2015

View full text Add to dashboard Cite

The study aimed to evaluate the antimicrobial susceptibility of 109 samples of Escherichia coli (E. coli) of environmental origin and to characterize these isolates according to the degree of pathogenicity in vivo, verifying a possible relationship between this variable and susceptibility to the active principles tested. The isolates were subjected to disc diffusion test to 14 antibiotics. From 16.5% to 90% of the samples were sensitive; 1 - 28.5% showed intermediate degree of susceptibility and between 9 to 78% of E. coli analyzed were resistant. The highest resistance percentages were seen in the class of quinolones and tetracyclines (>75%), and for sensitivity in the class of amphenicols (68.8%). By inoculating 1- day - old chicks, the isolates were classified as highly pathogenic (2.7%), intermediate (10.1%), low (42.2%) and apathogenic (45%). It was observed a wide variation in the susceptibility profile of isolates in relation to antimicrobials. It was also found that most of the samples had pathogenic potential (55%), thus being considered as APEC (avian pathogenic E. coli). No relationship between pathogenicity and antimicrobial susceptibility (P≤0.05) was observed.

show abstract

Characterization of Extraintestinal Escherichia coli Isolated from a Peacock (Pavo cristatus) with Colisepticemia

Cited by 12 publications

References 19 publications

Genotypes and Pathogenicity of Cellulitis Isolates Reveal Traits That Modulate APEC Virulence

Genotypes and Pathogenicity of Cellulitis Isolates Reveal Traits That Modulate APEC Virulence

Prevalence of ColV Plasmid-Linked Genes andIn VivoPathogenicity of Avian Strains ofEscherichia coli

Antimicrobial susceptibility and pathogenicity of Escherichia coli strains of environmental origin

Contact Info

Product

Resources

About