Characterization of fatty acid delta-6 desaturase gene in Nile tilapia and heterogenous expression in Saccharomyces cerevisiae

Tanomman, Supamas; Ketudat-Cairns, Mariena; Jangprai, Araya; Boonanuntanasarn, Surintorn

doi:10.1016/j.cbpb.2013.07.011

Cited by 55 publications

(47 citation statements)

References 49 publications

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“…Bifunctionality appears a relatively widespread feature among fish Fads2 as a consequence of sub- (acquisition of additional substrate specificities) and neo-functionalisation (substitution and/or acquisition of new substrate specificities) events that have occurred in teleost Fads2 6, 29 . More specifically, bifunctional ∆6∆5 Fads2 have been found in D. rerio 35 , S. canaliculatus 27 , O. niloticus 37 , C. estor 29 , C. gariepinus 38 and C. striata 39 . Moreover, all the ∆4 Fads2 found so far in fish also exhibited some ∆5 desaturase activity 27–30 , although none of them had ∆6 activity, which is consistent with the lack of ∆6 desaturase activity towards C 24 PUFA substrates observed in all the ∆4 Fads2 assayed in the present study.…”

Section: Discussionmentioning

confidence: 98%

Two alternative pathways for docosahexaenoic acid (DHA, 22:6n-3) biosynthesis are widespread among teleost fish

Oboh

Kabeya

Carmona‐Antoñanzas

et al. 2017

Sci Rep

113

108

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Docosahexaenoic acid (DHA) plays important physiological roles in vertebrates. Studies in rats and rainbow trout confirmed that DHA biosynthesis proceeds through the so-called “Sprecher pathway”, a biosynthetic process requiring a Δ6 desaturation of 24:5n−3 to 24:6n−3. Alternatively, some teleosts possess fatty acyl desaturases 2 (Fads2) that enable them to biosynthesis DHA through a more direct route termed the “Δ4 pathway”. In order to elucidate the prevalence of both pathways among teleosts, we investigated the Δ6 ability towards C24 substrates of Fads2 from fish with different evolutionary and ecological backgrounds. Subsequently, we retrieved public databases to identify Fads2 containing the YXXN domain responsible for the Δ4 desaturase function, and consequently enabling these species to operate the Δ4 pathway. We demonstrated that, with the exception of Δ4 desaturases, fish Fads2 have the ability to operate as Δ6 desaturases towards C24 PUFA enabling them to synthesise DHA through the Sprecher pathway. Nevertheless, the Δ4 pathway represents an alternative route in some teleosts and we identified the presence of putative Δ4 Fads2 in a further 11 species and confirmed the function as Δ4 desaturases of Fads2 from medaka and Nile tilapia. Our results demonstrated that two alternative pathways for DHA biosynthesis exist in teleosts.

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Section: Discussionmentioning

confidence: 98%

Two alternative pathways for docosahexaenoic acid (DHA, 22:6n-3) biosynthesis are widespread among teleost fish

Oboh

Kabeya

Carmona‐Antoñanzas

et al. 2017

Sci Rep

113

108

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“…Marine fi sh species, including Asian sea bass, Atlantic cod, and cobia, had the highest expression levels of LC-PUFA biosynthesis genes in brain ( 15,19,21 ). The relatively low ⌬ 6/ ⌬ 8 ratio of Fads2b, which was typical of marine species on one hand, and the higher expression of desaturase/elongase mRNA in liver, which was typical of freshwater species on the other, probably refl ected the three groups, including A. regius , T. thynnus , L. calcarifer , D. labrax , S. aurata , R. canadum (Percomorpharia), Psetta maxima (Carangimorphariae), and Oreochromis niloticus (Ovalentariae), have Fads2 enzymes functionally characterized as ⌬ 6 desaturases ( 11,16,19,21,26,27,29,30 ). However, investigation of desaturases from representatives of other phylogenetic branches is required to confi rm this hypothesis.…”

Section: Discussionmentioning

confidence: 99%

Diversification of substrate specificities in teleostei Fads2: characterization of Δ4 and Δ6Δ5 desaturases of Chirostoma estor

Fonseca-Madrigal

Navarro

Hontoria

et al. 2014

Journal of Lipid Research

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Biosynthesis of long-chain PUFAs (LC-PUFAs) in vertebrates involves sequential desaturation and elongation of C 18 PUFA, linoleic acid (LOA; 18:2n-6), and ␣ -linolenic acid (LNA; 18:3n-3) ( 1 ). Synthesis of arachidonic acid (ARA; 20:4n-6) and EPA (20:5n-3) from LOA and LNA, respectively, utilizes the same enzymes and pathways. The pre dominant pathway involves ⌬ 6 desaturation of LOA or LNA to 18:3n-6/18:4n-3 that are elongated to 20:3n-6/20:4n-3 followed by ⌬ 5 desaturation to ARA/EPA ( 1 ), but an alternative pathway with initial elongation of LOA or LNA followed by ⌬ 8 desaturation, an inherent ability of some ⌬ 6 desaturases, may be possible ( 2 ). Biosynthesis of DHA (22:6n-3) from EPA can also occur by two pathways. First, the so-called "Sprecher pathway" involves two sequential elongation steps from EPA to 24:5n-3 and a subsequent ⌬ 6 desaturation to 24:6n-3, followed by peroxisomal chain shortening ( 3 ). Second, a more direct pathway has been postulated in some marine fi sh that involves elongation of EPA to docosapentaenoic acid (22:5n-3) followed by ⌬ 4 desaturation to DHA ( 4,5 ).Dietary PUFAs are essential in fi sh, although requirements vary with species ( 6, 7 ). Generally, C 18 PUFAs can satisfy essential FA requirements of freshwater and salmonid species, but most marine fi sh have a requirement for LC-PUFAs such as EPA and DHA ( 8 ). Differing essential FA requirements have been linked to differences in the complement of fatty acyl desaturase (Fads) and elongase of very longchain FA (Elovl) genes ( 9-31 ). Thus, the dependence of Abstract Currently existing data show that the capability for long-chain PUFA (LC-PUFA) biosynthesis in teleost fi sh is more diverse than in other vertebrates. Such diversity has been primarily linked to the subfunctionalization that teleostei fatty acyl desaturase (Fads)2 desaturases have undergone during evolution. We previously showed that Chirostoma estor , one of the few representatives of freshwater atherinopsids, had the ability for LC-PUFA biosynthesis from C 18 PUFA precursors, in agreement with this species having unusually high contents of DHA. The particular ancestry and pattern of LC-PUFA biosynthesis activity of C. estor make this species an excellent model for study to gain further insight into LC-PUFA biosynthetic abilities among teleosts. The present study aimed to characterize cDNA sequences encoding fatty acyl elongases and desaturases, key genes involved in the LC-PUFA biosynthesis. Results show that C. estor expresses an elongase of very long-chain FA (Elovl)5 elongase and two Fads2 desaturases displaying ⌬ 4 and ⌬ 6/ ⌬ 5 specifi cities, thus allowing us to conclude that these three genes cover all the enzymatic abilities required for LC-PUFA biosynthesis from C 18 PUFA. In addition, the specifi cities of the C. estor Fads2 enabled us to propose potential evolutionary patterns and mechanisms for subfunctionalization of Fads2 among fi sh lineages. -GA-2010-276916, LONGFA). Additional funding was obtained from CONACYT, Mexico (INSAM FOINS 102/201...

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“…To date, Fads2 enzymes with Δ6 desaturase activities have been identified from freshwater fish including common carp ( Cyprinus carpio var. Jian) [11] and Nile tilapia ( Oreochromis niloticus ) [28], diadromous species such as Atlantic salmon ( Salmo salar ) [29], masu salmon ( Oncorhynchus masou ) [30] and meagre ( Argyrosomus regius ) [31], and marine fish including gilthead seabream [32], Atlantic cod ( Gadus morhua ) [33], cobia ( Rachycentron canadum ) [34], European sea bass [35], Asian sea bass ( Lates calcarifer ) [36], nibe croaker ( Nibea mitsukurii ) [37], Atlantic bluefin tuna ( Thunnus thynnus ) [38], striped snakehead fish ( Channa striata ) [39] and black seabream ( Acanthopagruss chlegeli ) [40]. In addition, a further Fads2 from Atlantic salmon was shown to be a monofunctional Δ5 desaturase [41], and bifunctional Δ6/Δ5 Fads2 enzymes were reported in the freshwater species zebrafish ( Danio rerio ) [42] and marine species rabbitfish [16].…”

Section: Discussionmentioning

confidence: 99%

Cloning, Functional Characterization and Nutritional Regulation of Δ6 Fatty Acyl Desaturase in the Herbivorous Euryhaline Teleost Scatophagus Argus

et al. 2014

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Marine fish are generally unable or have low ability for the biosynthesis of long-chain polyunsaturated fatty acids (LC-PUFA) from C18 PUFA precursors, with some notable exceptions including the herbivorous marine teleost Siganus canaliculatus in which such a capability was recently demonstrated. To determine whether this is a unique feature of S. canaliculatus or whether it is common to the herbivorous marine teleosts, LC-PUFA biosynthetic pathways were investigated in the herbivorous euryhaline Scatophagus argus. A putative desaturase gene was cloned and functionally characterized, and tissue expression and nutritional regulation were investigated. The full-length cDNA was 1972 bp, containing a 1338 bp open-reading frame encoding a polypeptide of 445 amino acids, which possessed all the characteristic features of fatty acyl desaturase (Fad). Functional characterization by heterologous expression in yeast showed the protein product of the cDNA efficiently converted 18:3n-3 and 18:2n-6 to 18:4n-3 and 18:3n-6, respectively, indicating Δ6 desaturation activity. Quantitative real-time PCR showed that highest Δ6 fad mRNA expression was detected in liver followed by brain, with lower expression in other tissues including intestine, eye, muscle, adipose, heart kidney and gill, and lowest expression in stomach and spleen. The expression of Δ6 fad was significantly affected by dietary lipid and, especially, fatty acid composition, with highest expression of mRNA in liver of fish fed a diet with a ratio of 18:3n-3/18:2n-6 of 1.72:1. The results indicated that S. argus may have a different LC-PUFA biosynthetic system from S. canaliculatus despite possessing similar habitats and feeding habits suggesting that LC-PUFA biosynthesis may not be common to all marine herbivorous teleosts.

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Characterization of fatty acid delta-6 desaturase gene in Nile tilapia and heterogenous expression in Saccharomyces cerevisiae

Cited by 55 publications

References 49 publications

Two alternative pathways for docosahexaenoic acid (DHA, 22:6n-3) biosynthesis are widespread among teleost fish

Two alternative pathways for docosahexaenoic acid (DHA, 22:6n-3) biosynthesis are widespread among teleost fish

Diversification of substrate specificities in teleostei Fads2: characterization of Δ4 and Δ6Δ5 desaturases of Chirostoma estor

Cloning, Functional Characterization and Nutritional Regulation of Δ6 Fatty Acyl Desaturase in the Herbivorous Euryhaline Teleost Scatophagus Argus

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