Characterization of FcεRI-bearing CD123+ blood dendritic cell antigen-2+ plasmacytoid dendritic cells in atopic dermatitis

Novak, Natalija; Allam, Jean‐Pierre; Hagemann, Tobias; Jenneck, Claudia; Laffer, Sylvia; Valenta, Rudolf; Kochan, Jarema; Bieber, Thomas

doi:10.1016/j.jaci.2004.05.038

Cited by 132 publications

(130 citation statements)

References 39 publications

Supporting

Mentioning

117

Contrasting

Unclassified

Order By: Relevance

“…PBMCs were prepared by Ficoll gradient centrifugation (Lymphoprep, Axis-Shield, Dundee, Scotland), as described previously (32), from buffy coats of human blood from healthy donors provided by the blood bank of the University of Bonn. Purification of PDCs was accomplished by a combination of negative and positive selection with immunomagnetic beads.…”

Section: Cell Purificationmentioning

confidence: 99%

“…Intracellular staining was performed as reported in detail elsewhere (32). MAbs used included PE-Cy5-labeled mAb against CD123 (9F5), PE-labeled mAb against IL-1b (8516.311), PE-labeled mAb against IL-6 (AS12), PE-labeled mAb against TNF-a (MAb11), and PE-labeled mouse IgG1k isotype control (MOPC-21), all purchased from BD Biosciences (Heidelberg, Germany).…”

Section: Intracellular Cytokine Stainingmentioning

confidence: 99%

See 1 more Smart Citation

Human Plasmacytoid Dendritic Cells Support Th17 Cell Effector Function in Response to TLR7 Ligation

Peng

Oldenburg

et al. 2009

The Journal of Immunology

Self Cite

100

View full text Add to dashboard Cite

Signals involved in the commitment of Th17 differentiation are of substantial interest for our understanding of antimicrobial defense mechanisms and autoimmune disorders. Various ways in which myeloid dendritic cells modulate Th17 differentiation have been identified. However, although plasmacytoid dendritic cells (PDCs) are regarded as important players in antiviral/antimicrobial host defense and autoimmune diseases, a putative modulatory role of PDCs in Th17 differentiation has not yet been elucidated in detail. We demonstrated that PDCs are capable of promoting Th17 differentiation in response to TLR7 stimulation. Further, both the differentiation of Th17 cells from naive T cells and the amplification of Th17 effector functions of memory T cells are promoted by PDCs after TLR7 activation. Our data are of strong clinical relevance because TLR7 activation in PDCs might represent one of the missing links between innate and adaptive immune mechanisms and contribute to the amplification of Th17-driven autoimmune disorders as well as viral host defense.

show abstract

Section: Cell Purificationmentioning

confidence: 99%

Section: Intracellular Cytokine Stainingmentioning

confidence: 99%

Human Plasmacytoid Dendritic Cells Support Th17 Cell Effector Function in Response to TLR7 Ligation

Peng

Oldenburg

et al. 2009

The Journal of Immunology

Self Cite

100

View full text Add to dashboard Cite

show abstract

“…Two major types of DC have been characterized in humans: the CD11c ϩ CD123 Ϫ myeloid (mDC, DC1), for which there are several subtypes, and the CD11c Ϫ CD123 ϩ lymphoid (DC2), also known as the plasmacytoid DC (pDC) (1). Studies have long supported the presence of the high-affinity IgE receptor (Fc⑀RI) on mDC, particularly those from allergic subjects, but have only recently identified this receptor on pDC (2,3). However, unlike the classical ␣␤␥ 2 tetramer that is uniquely expressed on basophils and mast cells and is involved in triggering mediator release from these cells, the role of the ␣␥ 2 variant on mDC and pDC is less clear, although the data at this time support it having a role in allergen presentation or "focusing" (4).…”

Section: Endritic Cells (Dc)mentioning

confidence: 99%

TLR9- and FcεRI-Mediated Responses Oppose One Another in Plasmacytoid Dendritic Cells by Down-Regulating Receptor Expression

Schroeder

Bieneman

Xiao

et al. 2005

The Journal of Immunology

142

160

View full text Add to dashboard Cite

Plasmacytoid dendritic cells (pDC) express not only TLR9 molecules through which ligation with CpG DNA favors Th1 responses but also possess IgE receptors (FcεRI) implicated in allergen presentation and induction of Th2 responses. This dichotomy prompted an investigation to determine whether TLR9- and IgE receptor-mediated responses oppose one another in pDC by affecting receptor expression and associated functional responses. Results showed that IgE cross-linking reduced TLR9 in pDC and inhibited the capacity of these cells to secrete IFN-α when stimulated with the CpG oligodeoxynucleotide (ODN)-2216. In contrast, an ∼15-fold reduction in FcεRIα mRNA and a loss in surface protein were seen in pDC first exposed to TLR9 ligation with ODN-2216. Results indicated that type I IFNs partly mediated this effect, as rIFN-α also caused a significant ∼4-fold reduction in FcεRIα mRNA. Finally, this reduction in FcεRIα mediated by ODN-2216 correlated with a selective suppression of allergen-induced CD4+ T cell proliferation, but not of responses resulting from tetanus toxoid. Overall, these results imply mechanisms by which specific innate and IgE-dependent immune responses counterregulate one another at the dendritic cell level and may have significant impact on whether an ensuing response is either of Th1 or Th2 in nature.

show abstract

“…It has been reported that low numbers of plasmacytoid DCs in the epidermal skin lesions of AD patients allows them to be highly prone to viral skin infections like herpes simplex-induced eczema herpeticum. 83 …”

Section: B Infectious Triggersmentioning

confidence: 99%

Clinical correlations of recent developments in the pathogenesis of atopic dermatitis

Sehra

Tuana

Holbreich

et al. 2008

An. Bras. Dermatol.

View full text Add to dashboard Cite

Atopic dermatitis is a chronic inflammatory skin disease with a steadily increasing prevalence affecting 10-20 % of infants and 1-3% of adults globally. It is often the first clinical manifestation of atopic disease preceding asthma and allergic rhinitis. Probably half of the children with atopic dermatitis develop some other form of atopic disease later in life. The pathogenesis involves a complex interplay of factors including genetic predisposition due to altered immune or skin barrier function, interactions with the environment such as food and allergen exposures, and infectious triggers of inflammation. In this review, we summarize the recent advances in understanding the contribution of different factors in the pathophysiology of atopic dermatitis and how insights provide new therapeutic potential for its treatment. Keywords: Asthma; Atopic Dermatitis, Eczema; Eosinophils; Genetics; Immunoglobulin E; Keratinocytes; Rhinitis, T-Lymphocytes Resumo: A dermatite atópica é uma doença cutânea inflamatória crônica cuja prevalência tem aumentado de forma constante, afetando 10-20% dos lactentes e 1-3% dos adultos em todo o mundo. Ela é freqüentemente a primeira manifestação clínica de doença atópica, precedendo a asma e a rinite alérgica. Provavelmente metade das crianças com dermatite atópica desenvolvem alguma

show abstract

Characterization of FcεRI-bearing CD123+ blood dendritic cell antigen-2+ plasmacytoid dendritic cells in atopic dermatitis

Cited by 132 publications

References 39 publications

Human Plasmacytoid Dendritic Cells Support Th17 Cell Effector Function in Response to TLR7 Ligation

Human Plasmacytoid Dendritic Cells Support Th17 Cell Effector Function in Response to TLR7 Ligation

TLR9- and FcεRI-Mediated Responses Oppose One Another in Plasmacytoid Dendritic Cells by Down-Regulating Receptor Expression

Clinical correlations of recent developments in the pathogenesis of atopic dermatitis

Contact Info

Product

Resources

About