Characterization of fibronectin interactions with glycosaminoglycans and identification of active proteolytic fragments.

Yamada, Kenneth M.; Kennedy, Dorothy W.; Kimata, Koji; Pratt, Robert M.

doi:10.1016/s0021-9258(18)43700-3

Cited by 395 publications

(64 citation statements)

References 52 publications

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“…It is uncertain why filaments were seen on ABM but not on CBM . Fibronectin has been shown to complex with heparan sulfate proteoglycans, promoting cell-substrate adhesions (23,24) . The apparent differences in filament distribution between ABM and CBM may therefore relate to the differences in heparan sulfate proteoglycan distribution discussed below.…”

Section: Discussionmentioning

confidence: 99%

Structural features of alveolar wall basement membrane in the adult rat lung.

Vaccaro¹,

Brody²

1981

The Journal of Cell Biology

139

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The ultrastructural characteristics of alveolar (ABM) and capillary (CBM) basement membranes in the adult rat lung have been defined using tannic acid fixation, ruthenium red staining, or incubation in guanidine HCI. ABM is dense and amorphous, has 3-to 5-nm filaments in the lamina rara externa (facing the alveolus) that run between the lamina densa and the basal cell surface of the epithelium, has an orderly array of ruthenium red-positive anionic sites that appear predominantly (79%) on the lamina rara externa, and has discontinuities beneath alveolar type II cells but not type I cells that allow penetration of type II cytoplasmic processes into the interstitium of the alveolar wall . The CBM is fibrillar and less compact than ABM, has no lamina rara filaments, and has one fifth the number of ruthenium red-positive anionic sites of ABM that appear predominantly (64%) overlying the lamina densa. Incubation of lung tissue with Flavobacterium heparinurn enzyme or with chondroitinase has shown that ABM anionic sites represent heparan sulfate proteoglycans, whereas CBM anionic sites contain this and other sulfated proteoglycans. The CBM fuses in a focal fashion with ABM, compartmentalizing the alveolar wall into a thick and thin side and establishing a thin, single, basement-membrane gas-exchange surface between alveolar air, and capillary blood. The potential implications of ABM and CBM ultrastructure for permeability, cell differentiation, and repair and morphogenesis of the lung are discussed.

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Section: Discussionmentioning

confidence: 99%

Structural features of alveolar wall basement membrane in the adult rat lung.

Vaccaro¹,

Brody²

1981

The Journal of Cell Biology

139

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“…Overall, our results demonstrated that both NHFB and CAF produced a higher amount of matrix bound HA when compared to the soluble counterpart. It has been hypothesized that HA is produced at the adhesion plaques [52] and might bind to the cellular fibronectin that is synthesized simultaneously [53,54]. In addition, TGF-β1 enhances the production of soluble HA in NHFB, while it increases matrix-bound HA in CAF, which appears to be incorporated into collagen fibrils.…”

Section: Nhfb and Caf Produced Matrix Bound Ha In Tgf-β1-dependent Mannermentioning

confidence: 99%

Matrix Remodeling and Hyaluronan Production by Myofibroblasts and Cancer-Associated Fibroblasts in 3D Collagen Matrices

Sapudom

Müller

Nguyen

et al. 2020

Gels

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The tumor microenvironment is a key modulator in cancer progression and has become a novel target in cancer therapy. An increase in hyaluronan (HA) accumulation and metabolism can be found in advancing tumor progression and are often associated with aggressive malignancy, drug resistance and poor prognosis. Wound-healing related myofibroblasts or activated cancer-associated fibroblasts (CAF) are assumed to be the major sources of HA. Both cell types are capable to synthesize new matrix components as well as reorganize the extracellular matrix. However, to which extent myofibroblasts and CAF perform these actions are still unclear. In this work, we investigated the matrix remodeling and HA production potential in normal human dermal fibroblasts (NHFB) and CAF in the absence and presence of transforming growth factor beta -1 (TGF-β1), with TGF-β1 being a major factor of regulating fibroblast differentiation. Three-dimensional (3D) collagen matrix was utilized to mimic the extracellular matrix of the tumor microenvironment. We found that CAF appeared to response insensitively towards TGF-β1 in terms of cell proliferation and matrix remodeling when compared to NHFB. In regards of HA production, we found that both cell types were capable to produce matrix bound HA, rather than a soluble counterpart, in response to TGF-β1. However, activated CAF demonstrated higher HA production when compared to myofibroblasts. The average molecular weight of produced HA was found in the range of 480 kDa for both cells. By analyzing gene expression of HA metabolizing enzymes, namely hyaluronan synthase (HAS1-3) and hyaluronidase (HYAL1-3) isoforms, we found expression of specific isoforms in dependence of TGF-β1 present in both cells. In addition, HAS2 and HYAL1 are highly expressed in CAF, which might contribute to a higher production and degradation of HA in CAF matrix. Overall, our results suggested a distinct behavior of NHFB and CAF in 3D collagen matrices in the presence of TGF-β1 in terms of matrix remodeling and HA production pointing to a specific impact on tumor modulation.

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“…The addition of heparin, to inhibit non-α & β " interactions [34][35][36][37], had a pronounced impact on levels induced both in the absence and in the presence of IL-1, decreasing total activity to levels averaging 30 % and 10 % of the total respectively (Figure 6b). Further, the decrease in IL-1-stimulated activity of the SAPK pathway in fibronectin-attached cells, corresponding to 35-50 %, was reversed in the presence of an RGD-containing peptide, resulting in levels similar to those in cells on tissue culture plastic.…”

Section: Figure 4 Il-1-induced Activity Of Sapk Is Inhibited By Fibronectin Attachmentmentioning

confidence: 99%

Regulation of interleukin 1 signalling through integrin binding and actin reorganization: disparate effects on NF-κB and stress kinase pathways

Zhu

Xiong

Rodgers

et al. 1998

Biochemical Journal

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Interleukin 1 (IL-1)-mediated gene regulation is dependent on cell-matrix interactions. Both IL-1-activated pathways, nuclear factor kappaB (NF-kappaB) and the stress-activated protein kinase (SAPK), can be regulated by cell adhesion and changes in the cytoskeleton, suggesting that cell-matrix effects on IL-1 responses are initiated in part though effects on signal transduction. Here we show that IL-1-induced transient alterations in cell shape and in the cytoskeleton in fibronectin attached cells are correlated with effects on peak activity of NF-kappaB and SAPK. Cells on fibronectin showed a 1.5-2-fold enhancement in IL-1-induced NF-kappaB activity compared with levels in cells on poly(l-lysine) or bare tissue culture plates. The effect was increased with increasing concentrations of fibronectin and was most prominent at lower concentrations of IL-1. In contrast, fibronectin attachment caused an approx. 50% decrease in the IL-1 activation of SAPK, eliminating the peak activity after 15 min of stimulation with IL-1. IL-1-induced NF-kappaB activity showed a successive, substratum-independent increase during 4 h of attachment and spreading, whereas the inhibitory effect of fibronectin on the SAPK pathway was induced at the initial stages of attachment. Further, the addition of a peptide containing the motif RGD resulted in a 40% decrease in NF-kappaB activity in cells on fibronectin, largely accounted for by an effect on the p50/p65 heterodimer. Similarly, blocking of integrin aggregation by RGD-containing peptide resulted in a total abrogation of the fibronectin effect on IL-1-induced SAPK activity. The results demonstrate disparate effects of cell adhesion on the activation by IL-1 of the NF-kappaB and SAPK pathways. Thus fibronectin attachment causes an up-regulation of NF-kappaB activity in the presence of IL-1, whereas in contrast it results in a pronounced decrease in IL-1-induced SAPK activity.

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Characterization of fibronectin interactions with glycosaminoglycans and identification of active proteolytic fragments.

Cited by 395 publications

References 52 publications

Structural features of alveolar wall basement membrane in the adult rat lung.

Structural features of alveolar wall basement membrane in the adult rat lung.

Matrix Remodeling and Hyaluronan Production by Myofibroblasts and Cancer-Associated Fibroblasts in 3D Collagen Matrices

Regulation of interleukin 1 signalling through integrin binding and actin reorganization: disparate effects on NF-κB and stress kinase pathways

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