Characterization of FimA in<i>Porphyromonas gingivalis</i>genotype IV

Choi, Young-Suk; Moon, Ji-Hoi; Park, Jaehong; Lee, Jin‐Yong

doi:10.1111/j.1574-695x.2012.00955.x

Cited by 5 publications

(4 citation statements)

References 12 publications

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“…Our negative control strain YPF1, a 33277 fimA deletion mutant, as expected, was devoid of detectable amounts of FimA. We were unable to detect FimA IV protein from strain W83 (IV), which is not surprising, as previous reports have shown that levels of expression are low in the this strain, and it is antigenically distinct from the type I protein, thus less reactive with FimA I antibody [54], [55]. Interestingly, while expression of FimA IV protein in strain W83 was undetectable, allelic exchange mutant A4 produced detectable amounts of FimA IV protein, implying that much higher levels of this protein are being expressed in the 33277 background than in the W83 background.…”

Section: Resultssupporting

confidence: 66%

Genetic Exchange of Fimbrial Alleles Exemplifies the Adaptive Virulence Strategy of Porphyromonas gingivalis

et al. 2014

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Porphyromonas gingivalis is a gram–negative anaerobic bacterium, a member of the human oral microbiome, and a proposed “keystone” pathogen in the development of chronic periodontitis, an inflammatory disease of the gingiva. P. gingivalis is a genetically diverse species, and is able to exchange chromosomal DNA between strains by natural competence and conjugation. In this study, we investigate the role of horizontal DNA transfer as an adaptive process to modify behavior, using the major fimbriae as our model system, due to their critical role in mediating interactions with the host environment. We show that P. gingivalis is able to exchange fimbrial allele types I and IV into four distinct strain backgrounds via natural competence. In all recombinants, we detected a complete exchange of the entire fimA allele, and the rate of exchange varies between the different strain backgrounds. In addition, gene exchange within other regions of the fimbrial genetic locus was identified. To measure the biological implications of these allele swaps we compared three genotypes of fimA in an isogenic background, strain ATCC 33277. We demonstrate that exchange of fimbrial allele type results in profound phenotypic changes, including the quantity of fimbriae elaborated, membrane blebbing, auto-aggregation and other virulence-associated phenotypes. Replacement of the type I allele with either the type III or IV allele resulted in increased invasion of gingival fibroblast cells relative to the isogenic parent strain. While genetic variability is known to impact host-microbiome interactions, this is the first study to quantitatively assess the adaptive effect of exchanging genes within the pan genome cloud. This is significant as it presents a potential mechanism by which opportunistic pathogens may acquire the traits necessary to modify host-microbial interactions.

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Section: Resultssupporting

confidence: 66%

Genetic Exchange of Fimbrial Alleles Exemplifies the Adaptive Virulence Strategy of Porphyromonas gingivalis

et al. 2014

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“…Pili (or fimbriae) are filamentous structures on the bacterial cell surface, with crucial role in virulence and host cell attachment (Yoshimura et al, 2009;Ellison et al, 2022). The pathogenic role of type IV pilins has been reported for the periodontitis associated bacteria P. gingivalis (Choi et al, 2012) and Eikenella corrodens (Hood and Hirschberg, 1995), and their enhanced expression under periodontal disease conditions has been previously reported (Szafrański et al, 2015). Transcription of flagellin from Treponema was also enhanced in periodontal disease.…”

Section: Discussionmentioning

confidence: 99%

Microbial signatures in human periodontal disease: a metatranscriptome meta-analysis

Ovsepian,

Kardaras,

Skoulakis

et al. 2024

Front. Microbiol.

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The characterization of oral microbial communities and their functional potential has been shaped by metagenomics and metatranscriptomics studies. Here, a meta-analysis of four geographically and technically diverse oral shotgun metatranscriptomics studies of human periodontitis was performed. In total, 54 subgingival plaque samples, 27 healthy and 27 periodontitis, were analyzed. The core microbiota of the healthy and periodontitis group encompassed 40 and 80 species, respectively, with 38 species being common to both microbiota. The differential abundance analysis identified 23 genera and 26 species, that were more abundant in periodontitis. Our results not only validated previously reported genera and species associated with periodontitis with heightened statistical significance, but also elucidated additional genera and species that were overlooked in the individual studies. Functional analysis revealed a significant up-regulation in the transcription of 50 gene families (UniRef-90) associated with transmembrane transport and secretion, amino acid metabolism, surface protein and flagella synthesis, energy metabolism, and DNA supercoiling in periodontitis samples. Notably, the overwhelming majority of the identified gene families did not exhibit differential abundance when examined across individual datasets. Additionally, 4 bacterial virulence factor genes, including TonB dependent receptor from P. gingivalis, surface antigen BspA from T. forsynthia, and adhesin A (PsaA) and Type I glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from the Streptococcus genus, were also found to be significantly more transcribed in periodontitis group. Microbial co-occurrence analysis demonstrated that the periodontitis microbial network was less dense compared to the healthy network, but it contained more positive correlations between the species. Furthermore, there were discernible disparities in the patterns of interconnections between the species in the two networks, denoting the rewiring of the whole microbial network during the transition to the disease state. In summary, our meta-analysis has provided robust insights into the oral active microbiome and transcriptome in both health and disease.

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“…It is known that P. gingivalis fimbriae are highly immunogenic and immunization with purified fimbria from P. gingivalis 381 inhibits the progression of periodontal disease. 37 Fimbriae are responsible for the adhesion and invasion of bacteria that can cause periodontal tissue damage. Some researchers have made it a vaccine candidate, because of its ability to facilitate adhesion and invasion of bacteria into tissues.…”

Section: Discussionmentioning

confidence: 99%

The Role of Porphyromonas gingivalis Virulence Factors in Periodontitis Immunopathogenesis

Septiwidyati

Bachtiar

2020

Dentika Dental J.

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Porphyromonas gingivalis is an anaerobic Gram-negative oral bacterium involved in the pathogenesis of periodontitis. Periodontitis is an infection that is characterized by damage to the supporting tissues of the teeth so that it can cause tooth loss if not given treatment. P. gingivalis locally can invade periodontal tissue and avoid host defense mechanisms. This bacterium has virulence factors which can cause deregulation of innate immune responses and inflammation in the host. The role of P. gingivalis virulence factors such as capsules, fimbriae, lipopolysaccharides, and gingipain in the pathogenesis of periodontitis will be discussed in this paper.

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Characterization of FimA inPorphyromonas gingivalisgenotype IV

Cited by 5 publications

References 12 publications

Genetic Exchange of Fimbrial Alleles Exemplifies the Adaptive Virulence Strategy of Porphyromonas gingivalis

Genetic Exchange of Fimbrial Alleles Exemplifies the Adaptive Virulence Strategy of Porphyromonas gingivalis

Microbial signatures in human periodontal disease: a metatranscriptome meta-analysis

The Role of Porphyromonas gingivalis Virulence Factors in Periodontitis Immunopathogenesis

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