“…This caused severe problems in performing the oil extraction from wet microalgae, since the oil-containing microalgae were located in dilute solutions containing microalgae fragments that were difficult to concentrate. To cope with this problem, the present study tried to utilize the properties of the cell wall-associated polysaccharides of microalgae to induce flocculation and precipitation of the microalgal biomass by treating it with a sufficient amount of methanol (Guo et al, 2013;Xu et al, 2014). After the microwave disruption and methanol flocculation steps, the concentrated microalgae biomass with a solid content of 56.6-60.5% could be easily and effectively collected using a commercial filtration bag and a spin dryer, thus significantly reducing the volume of the microalgae feedstock for the subsequent oil extraction.…”