Characterization of Four New Rhinovirus Serotypes

Ca, Phillips; Jl, Melnick; Sullivan, Lacey

doi:10.3181/00379727-134-34914

Cited by 6 publications

(3 citation statements)

References 7 publications

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“…The fact that four reciprocal and five one-way cross-reactions were observed in the 37 rhinoviruses studied suggests that a substantially greater number of cross-reactions will be observed among the 90 (including rhinovirus 1B) presently classified viruses (1). There are undoubtedly caiididate rhinoviruses which will increase this number (13). A simple comparison of the areas (372 versus 902) of the matrices of antisera and viruses suggests that perhaps six times as many crosses might be observed in the larger matrix than we have observed in our present small matrix of 37 by 37.…”

Section: Resultsmentioning

confidence: 49%

Cross Relationships Among 37 Rhinoviruses Demonstrated by Virus Neutralization with Potent Monotypic Rabbit Antisera

et al. 1973

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Antisera produced in rabbits to 37 rhinovirus (RV) types have been examined for antibody to the 36 corresponding heterologous types. Reciprocal neutralization was noted between RV types 2 and 49 and RV types 13 and 41. Five additional monotypic rhinoviral rabbit antisera neutralized one heterologous rhinovirus. Neutralizing antibody titers against heterologous RV serotypes were similar to those shown in the RV 1A, 1B and RV 9, 32 reciprocal cross-reactions, and would not be expected to result in false identification of serotypes. Comparison of neutralizing antibody titers and neutralization rate constants in serial serum specimens of immunized rabbits showed that antibody response to the immunizing RV type and the cross-reacting type followed a similar time pattern.

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Section: Resultsmentioning

confidence: 49%

Cross Relationships Among 37 Rhinoviruses Demonstrated by Virus Neutralization with Potent Monotypic Rabbit Antisera

et al. 1973

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“…Resistance to repeated illnesses by any one of the cightynine different serotypes (3) is due to typespecific antibody at the nasal membrane silrfaces (4,5). Recovery from a rhinovirus infection is followed by a transient period of nonspecific resistance to subsequent infection and establishment of URI by heterologous rhinoviruses ; the period of nonspecific resistance can last for several weeks (6)(7)(8).…”

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confidence: 99%

Interferon Induction by Rhinoviruses and Effect of Interferon on Rhinovirus Yields in Human Cell Lines

Stoker

Kiernat

Gauntt

1973

Experimental Biology and Medicine

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confidence: 99%

Growth of rhinovirus type 14

Gauntt

Sauck

Carlson

1973

Archiv f Virusforschung

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The rhinoviruses, a subgroup within the pieornaviruses, currently consist of 89 different serotypes (8). A number of differences in biological properties have been reported for the few rhinovirus serotypes studied (see review, 10), however, additional data are needed to warrant continued classification of this large a group of viruses within one subgroup. In this study, we describe some biological characteristics of the growth of rhinovirus type 14 (RV 14) in IteL~ and KB cell cultures.Methods for the propagation and assay of RV14 and for the culture and preparation of KB and HeLa cells for experiments were previously described (9). Zero time in all studies was regarded as the instant of addition of virus to cell cultures.l~ates of adsorption of RV14 to KB and HeLa monolayer cell cultures at 34.5~ C were measured by determining the amount of virus remMning in an inoeulum after challenge of cells with 10 plaque-forming units (PFU)/cell in 0.2 ml. Virus adsorption to KB cell cultures was greater (55 per cent) than to HeLa cell cultures (36 per cent) within the initial 30 minute period. Thereafter, similar rates of adsorption occurred to either cell culture and adsorption was approximately linear for the next 90 minutes, resulting in adsorption of 95 and 79 per cent of virus to KB and HeLa cell cultures, respectively. In published studies, only 30 to 35 per cent of t~V 14 adsorbed to tteLa cells in suspension culture and adsorption was essentially complete by 30 minutes post inoculation (p.i.) (6, 11). Under comparable conditions, rhinovirus type 2 (RV2) attached at a-rate which was 20 to 40 times greater than that of RV 14 (6).The time course of production of infectious RV 14 in KB and HeLa cell cultures following challenge with 20 to 25 PFU/eell is shown in Fig. 1. The growth cycle of I~V 14 in KB cells required only two-thirds as much time as a complete growth cycle in HeLa cells. Eclipse and rise periods in KB cells were 1 and 2 to 3 hours shorter, respectively, than similar periods in infected HeLa cells: Approximately 1 per cent and between 5 and 8 per cent of infectious virus were released as free

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Characterization of Four New Rhinovirus Serotypes

Cited by 6 publications

References 7 publications

Cross Relationships Among 37 Rhinoviruses Demonstrated by Virus Neutralization with Potent Monotypic Rabbit Antisera

Cross Relationships Among 37 Rhinoviruses Demonstrated by Virus Neutralization with Potent Monotypic Rabbit Antisera

Interferon Induction by Rhinoviruses and Effect of Interferon on Rhinovirus Yields in Human Cell Lines

Growth of rhinovirus type 14

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