Characterization of fructan oligomers from species of the genus Allium L.

Ernst, Michael; Chatterton, N. J.; Harrison, P. A.; Matitschka, Gudrun

doi:10.1016/s0176-1617(98)80044-8

Cited by 75 publications

(57 citation statements)

References 20 publications

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“…These newly produced fructooligosaccharides were also isolated from chicory roots [18]. Similar inulon-ose series of DP 2 and DP 3 of fructose polymers were identified in both sprouted and unsprouted onion bulbs after two-month storage [19]. However, although the degree of polymerization was determined, these authors did not report indeed the structural conformation of the fructosyl terminal of these isolated saccharides as reported in the present investigation.…”

Section: Resultssupporting

confidence: 57%

“…Later, Timmermans et al [18] confirmed the presence of three new series of oligosaccharides in forced chicory roots (one disaccharide, one trisaccharide, and one tetrasaccharide) which contain neither β(2 → 1) nor β(2 → 6) linkages. Ernst et al [19] reported later the presence of two inulo-n-ose series without a terminal glucose from species of the Allium genus.…”

Section: Introductionmentioning

confidence: 99%

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Isolation and Structural Analysis In Vivo of Newly Synthesized Fructooligosaccharides in Onion Bulbs Tissues (Allium cepa L.) during Storage

Fujishima

Furuyama

Ishihiro

et al. 2009

International Journal of Carbohydrate Chemistry

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Recommended by Thomas HeinzeFructooligosaccharides are involved in physiological activities and quality attributes of onion bulbs. This work describes structures of newly synthesized oligosaccharides formed by fructose moieties in onion bulb tissues during storage. Onion bulbs were stored for four weeks at 10 • C. HPAEC-PAD analysis showed that saccharide 1 was eluted after 1-kestose while saccharide 2 was eluted after nystose (4a). Saccharides 1 and 2 have R-sucrose values of 1.55 and 2.15 by HPAEC, a reducing terminal, a reducing sugar-to-fructose ratio of 0.5 and 0.3, and a degree of polymerization of 2 and 3 by TOF-MS, respectively. GLC analysis of the methyl derivatives and NMR measurement of the saccharides confirmed the presence of two different structures: the structure of saccharide 1 is composed by two fructose moieties and linked by β(2 → 1) linkage and was identified as inulobiose [β-Dfructofuranosyl-(2 → 1)-β-D-fructopyranose]. The structure of saccharide 2 consists of three units of fructose linked by β(2 → 1) linkage and was identified as inulotriose [β-D-The spectra also showed that 70 to 80% of the terminal fructose residue of the two saccharides is of pyranosyl form, while 20 to 30% is of furanosyl form. This finding demonstrated that these newly produced saccharides, catalyzed by onion-purified 6G-FFT, were synthesized by the action of 1-FFT fructosyltransfer from 1-kestose to free fructopyranose yielding inulobiose and sucrose, while elongation of fructofuranosyl units occurs at this transferred fructofuranosyl residue to produce inulooligosaccharide having an additional unit of fructofuranose.

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Section: Resultssupporting

confidence: 57%

Section: Introductionmentioning

confidence: 99%

Isolation and Structural Analysis In Vivo of Newly Synthesized Fructooligosaccharides in Onion Bulbs Tissues (Allium cepa L.) during Storage

Fujishima

Furuyama

Ishihiro

et al. 2009

International Journal of Carbohydrate Chemistry

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“…This complex is rich in oligosaccharide, pectin and other substances and recently has been recognized as a effective in treating digestion, wounds, festering sores and cholesterol, among other ailments (Inden and Asahira, 1990). In bunching onion, a limited number of studies have characterized fructose-oligosaccharides in the leaf sheath (Ernst et al, 1998). The result of a preliminary TLC analysis showed that monosaccharides to pentasaccharides were contained in the 70% ethanol extract from a leaf blade.…”

Section: Resultsmentioning

confidence: 99%

Effect of single alien chromosome from shallot (Allium cepa L. Aggregatum group) on carbohydrate production in leaf blade of bunching onion (A. fistulosum L.)

et al. 2004

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We used a complete set of Allium fistulosum -shallot ( A. cepa Aggregatum group) monosomic addition lines (FF+1A -FF+8A) to identify shallot chromosomes affecting the production of sugars. In the alien addition lines grown over two years in an experimental field at Yamaguchi University (34 ° N, 131 ° E), shallot chromosomes 2A and 8A altered sugar contents in leaf-bunching onion ( A. fistulosum ). Except for FF+2A, every monosomic addition accumulated nonreducing sugars in winter leaf blades. FF+8A caused an increase in the amounts of non-reducing sugars in the winter. FF+2A hardly produced non-reducing sugar throughout the two-year study. These results indicated that genes related to non-reducing sugar metabolism are located on the 2A and 8A chromosomes. The results of regression analyses using 2002 data on A. fistulosum and the monosomic addition set revealed a correlation (r = 0.63 ± 0.07; mean ± SE., n = 9) between reducing sugar and monosaccharide (Glc+Fru) contents but no correlation between non-reducing sugar and sucrose contents. This result indicates the existence of other polysaccharides (e.g., scorodose) as non-reducing sugars in the leaf blade.

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“…It has been noted that content of low DP FOS is correlated to that of dry matter (DM 10%), 28) while in high dry matter onion bulbs, the maximum degree of polymerization is between 10 and 15. 8, 29) 2. Anabolism of FOS in onion bulb tissues.…”

Section: Fructooligosaccharides In Onion Bulbsmentioning

confidence: 99%

The Metabolism of the Fructooligosaccharides in Onion Bulbs: A Comprehensive Review

2005

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In the past two decades, there has been vast expansion in the research of fructooligosaccharides (FOS), including their chemistry, biochemistry and enzymology in higher plants. However, in spite of these considerable advances in fructan science, many other aspects of the mechanisms of fructan metabolism have not been fully understood. The bulbing and yield of dry onion cultivars vary, but they depend on the contents of the high dry matter and non-structural carbohydrates (fructooligosaccharides) which contribute to keepability. The knowledge of the mechanisms of the synthesis and the degradation of the FOS occurring during growth and storage are of great interest. Important progress has been made in the research area of onion FOS, and in addition to their role as accessible reserve carbohydrates in bulbs during sprouting, FOS participate in many physiological processes of production, protection and preservation of the bulbs. This review aims to contribute to a better understanding of the metabolism of FOS in onion bulbs, based on recent investigations. The activities of the main enzymes involved in the synthesis and the hydrolysis of the FOS during the pre-harvest (growth) and post-harvest (storage) life of the bulbs are reviewed, although present knowledge is too limited to explain clearly the mechanisms triggering the enzymes activities or the mechanisms by which FOS contribute to quality and long keepability of the bulbs.

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Characterization of fructan oligomers from species of the genus Allium L.

Cited by 75 publications

References 20 publications

Isolation and Structural Analysis In Vivo of Newly Synthesized Fructooligosaccharides in Onion Bulbs Tissues (Allium cepa L.) during Storage

Isolation and Structural Analysis In Vivo of Newly Synthesized Fructooligosaccharides in Onion Bulbs Tissues (Allium cepa L.) during Storage

Effect of single alien chromosome from shallot (Allium cepa L. Aggregatum group) on carbohydrate production in leaf blade of bunching onion (A. fistulosum L.)

The Metabolism of the Fructooligosaccharides in Onion Bulbs: A Comprehensive Review

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