Characterization of fructooligosaccharide-degrading enzymes in human commensal Bifidobacterium longum and Anaerostipes caccae

Tanno, Hiroki; Fujii, Tadashi; Ose, Riichi; Hirano, Katsuaki; Tochio, Takumi; Endo, Akihito

doi:10.1016/j.bbrc.2019.08.049

Cited by 17 publications

(11 citation statements)

References 26 publications

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“…Bifidobacterium used MFS transporter to transport FOS and B. longum SB22 had similar gene clusters of FOS utilization to that in the previous study. 28 Likewise, the gene clusters of FOS utilization in L. paracasei were similar to L. paracasei 1195 as reported by Goh et al 10 In addition, S. alactolyticus had the same gene clusters of FOS utilization with Lactobacillus plantarum WCFS1. 29 Both Lactobacillus and Enterococcus species utilize the PTS system to transport FOS.…”

Section: Discussionsupporting

confidence: 75%

Bifidobacteria exhibited stronger ability to utilize fructooligosaccharides, compared with other bacteria in the mouse intestine

Mao

Cui

et al. 2021

J Sci Food Agric

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BACKGROUND: Fructooligosaccharides (FOS) have been identified as important prebiotics. Previous studies have found that they can significantly promote the proliferation of Bifidobacterium pseudolongum in the mouse intestine. However, it is still unclear which other bacteria in the mouse intestine can utilize FOS, and the differences in the ability to utilize FOS. In this study, the bacteria capable of utilizing FOS were isolated from mice feces and their ability to utilize FOS was compared. Draft genome sequencing was also applied to explain the differences in FOS utilization at the gene levels.RESULTS: A total of 15 species were isolated from mouse feces and 13 species were able to utilize fructofuranosylnystose (GF2). Eleven species could utilize nistose (GF3), but not Enterococcus hirae and Lactobacillus reuteri. In contrast, 1-kestose (GF4) was hardly utilized. The enzyme activity determination and draft genome sequencing-based analyses revealed that all isolated species used the phosphotransferase system or permease system to transport FOS into the cells before hydrolysis by ⊎-fructofuranosidase. Although ⊎-fructofuranosidase exists in all strains, there are big differences in the corresponding coding genes between bifidobacteria and non-bifidobacteria.CONCLUSION: Compared with the other isolates, Bifidobacterium species exhibited higher enzyme activity and shorter generation time, leading to a stronger ability to utilize FOS.

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Section: Discussionsupporting

confidence: 75%

Bifidobacteria exhibited stronger ability to utilize fructooligosaccharides, compared with other bacteria in the mouse intestine

Mao

Cui

et al. 2021

J Sci Food Agric

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“…DP4 nystose is usually the major component of commercialized FOS [ 13 , 20 ], whereas DP3 1-kestose showed better growth promoting activity in bifidobacteria and butyrate-producing human commensal Anaerostipes caccae than nystose [ 41 ]. The different activity was due to absence of genes involved in nystose-hydrolyzing enzymes and/or poor gene induction activity of nystose in the organisms [ 42 ]. Thus, FOS, especially in 1-kestose, is a promising prebiotic to alleviate insulin resistance-related disorders in obese-prone adults.…”

Section: Discussionmentioning

confidence: 99%

Supplementation of 1-Kestose Modulates the Gut Microbiota Composition to Ameliorate Glucose Metabolism in Obesity-Prone Hosts

Watanabe

Tochio

Kadota³

et al. 2021

Nutrients

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Insulin resistance leads to the onset of medical conditions such as type 2 diabetes, and its development is associated with the alteration in the gut microbiota. Although it has been demonstrated that supplementation with prebiotics modulates the gut microbiota, limited evidence is available for effects of prebiotics on insulin resistance, especially for humans. We investigated the prebiotic effect of 1-kestose supplementation on fasting insulin concentration in obesity-prone humans and rats. In the preliminary study using rats, the hyperinsulinemia induced by high-fat diet was suppressed by intake of water with 2% (w/v) 1-kestose. In the clinical study using obese-prone volunteers, the fasting serum insulin level was significantly reduced from 6.5 µU/mL (95% CI, 5.5–7.6) to 5.3 (4.6–6.0) by the 12-week intervention with supplementation of 10 g 1-kestose/day, whereas it was not changed by the intervention with placebo (6.2 µU/mL (5.4–7.1) and 6.5 (5.5–7.6) before and after intervention, respectively). The relative abundance of fecal Bifidobacterium was significantly increased to 0.3244 (SD, 0.1526) in 1-kestose-supplemented participants compared to that in control participants (0.1971 (0.1158)). These results suggest that prebiotic intervention using 1–kestose may potentially ameliorate insulin resistance in overweight humans via the modulation of the gut microbiota. UMIN 000028824.

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“…This study also found multiple FOS utilization gene clusters in bifidobacteria. FOS-C1 and FOS-C2 have been characterized in several strains [ 58 , 59 ]. FOS-C3/C4 seems to represent species-specific clusters of B. breve genes, and its function needs to be characterized.…”

Section: Discussionmentioning

confidence: 99%

Gene–Phenotype Associations Involving Human-Residential Bifidobacteria (HRB) Reveal Significant Species- and Strain-Specificity in Carbohydrate Catabolism

et al. 2021

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Bifidobacteria are among the first colonizers of the human gastrointestinal tract. Different bacterial species use different mechanisms for utilization of various carbon sources in order to establish themselves in the complex microbial ecosystem of the gut. However, these mechanisms still need to be explored. Here, a large gene–phenotype correlation analysis was carried out to explore the metabolic and genetic diversity of bifidobacterial carbohydrate utilization abilities. In this study, we used 21 different carbohydrates to determine the growth phenotypes, the distribution of glycoside hydrolases (GHs), and gene clusters related to the utilization of multiple carbon sources in six human-residential Bifidobacterium species. Five carbohydrates significantly stimulated growth of almost all strains, while the remaining sugars exhibited species- and strain-specificity. Correspondingly, different Bifidobacterium species also had specific GHs involved in fermentation of plant or host glycans. Moreover, we analyzed several carbohydrate utilization gene clusters, such as 2-fucosyllactose (2′FL), sialic acid (SA), and fructooligosaccharide (FOS). In summary, by using 217 bifidobacterial strains and a wide range of growth substrates, our research revealed inter- and intra-species differences in bifidobacterial in terms of carbohydrate utilization. The findings of this study are useful for the process of developing prebiotics for optimum growth of probiotics, especially Bifidobacterium species.

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Characterization of fructooligosaccharide-degrading enzymes in human commensal Bifidobacterium longum and Anaerostipes caccae

Cited by 17 publications

References 26 publications

Bifidobacteria exhibited stronger ability to utilize fructooligosaccharides, compared with other bacteria in the mouse intestine

Bifidobacteria exhibited stronger ability to utilize fructooligosaccharides, compared with other bacteria in the mouse intestine

Supplementation of 1-Kestose Modulates the Gut Microbiota Composition to Ameliorate Glucose Metabolism in Obesity-Prone Hosts

Gene–Phenotype Associations Involving Human-Residential Bifidobacteria (HRB) Reveal Significant Species- and Strain-Specificity in Carbohydrate Catabolism

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