Characterization of fungi isolated from the equipment used in the International Space Station or Space Shuttle

Satoh, Kazuo; Yamazaki, Takashi; Nakayama, Takako; Umeda, Yoshiko; Alshahni, Mohamed Mahdi; Makimura, Mizue; Makimura, Koichi

doi:10.1111/1348-0421.12375

Cited by 27 publications

(19 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(2015) in the USA. This species was also reported by Cruywagen et al (2015) on baobab trees in southern Africa, and on equipment used in the International Space Station or Space Shuttle in Japan (Satoh et al . 2016).…”

Section: Introductionsupporting

confidence: 62%

“…Substrate and distribution : Isolated from mouldy paint, Suriname (Crous et al 2007); ancient laid-paper documents, Portugal (Mesquita et al 2009); associated with patients with atopic dermatitis, Japan (Zhang et al 2011); colonizing tattoo inks, Italy (Bonadonna et al 2014); on parchment manuscripts, Italy (Piñar et al 2015); from human blood and finger nail, USA (Sandoval-Denis et al 2015); on Adansonia digitata , South Africa (Cruywagen et al 2015); and on equipment used in the International Space Station or Space Shuttle, Japan (Satoh et al 2016). …”

Section: Taxonomymentioning

confidence: 99%

See 1 more Smart Citation

New endophytic Toxicocladosporium species from cacti in Brazil, and description of Neocladosporium gen. nov.

et al. 2017

View full text Add to dashboard Cite

Brazil harbours a unique ecosystem, the Caatinga, which belongs to the tropical dry forest biome. This region has an important diversity of organisms, and recently several new fungal species have been described from different hosts and substrates within it. During a survey of fungal endophyte diversity from cacti in this forest, we isolated cladosporium-like fungi that were subjected to morphological and multigene phylogenetic analyses including actA, ITS, LSU, rpb2 and tub2 gene sequences. Based on these analyses we identified two new species belonging to the genus Toxicocladosporium, described here as T. cacti and T. immaculatum spp. nov., isolated from Pilosocereus gounellei subsp. gounellei and Melocactus zehntneri, respectively. To improve the species recognition and assess species diversity in Toxicocladosporium we studied all ex-type strains of the genus, for which actA, rpb2 and tub2 barcodes were also generated. After phylogenetic reconstruction using five loci, we differentiated 13 species in the genus. Toxicocladosporium velox and T. chlamydosporum are synonymized based on their phylogenetic position and limited number of unique nucleotide differences. Six strains previously assigned to T. leucadendri, including the ex-type strain (CBS 131317) of that species, were found to belong to an undescribed genus here named as Neocladosporium gen. nov., with N. leucadendri comb. nov. as type species. Furthermore, this study proposes the actA, ITS, rpb2 and tub2 as main phylogenetic loci to recognise Toxicocladosporium species.

show abstract

Section: Introductionsupporting

confidence: 62%

Section: Taxonomymentioning

confidence: 99%

New endophytic Toxicocladosporium species from cacti in Brazil, and description of Neocladosporium gen. nov.

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Surfaces onboard the ISS should of course not be exposed to nutrient rich liquid over such a long timeframe as it was done in this experiment, but our findings emphasize that local moisture has to be kept down to a minimum to prevent potentially harmful biofilm formation. This is additionally important with respect to fungal growth, as these might affect human health indirectly by causing allergic reactions and asthmatic responses 18,43–45 .…”

Section: Discussionmentioning

confidence: 99%

Space Station conditions are selective but do not alter microbial characteristics relevant to human health

Mora

Wink

et al. 2019

Nat Commun

View full text Add to dashboard Cite

The International Space Station (ISS) is a unique habitat for humans and microorganisms. Here, we report the results of the ISS experiment EXTREMOPHILES, including the analysis of microbial communities from several areas aboard at three time points. We assess microbial diversity, distribution, functional capacity and resistance profile using a combination of cultivation-independent analyses (amplicon and shot-gun sequencing) and cultivation-dependent analyses (physiological and genetic characterization of microbial isolates, antibiotic resistance tests, co-incubation experiments). We show that the ISS microbial communities are highly similar to those present in ground-based confined indoor environments and are subject to fluctuations, although a core microbiome persists over time and locations. The genomic and physiological features selected by ISS conditions do not appear to be directly relevant to human health, although adaptations towards biofilm formation and surface interactions were observed. Our results do not raise direct reason for concern with respect to crew health, but indicate a potential threat towards material integrity in moist areas.

show abstract

“…condensate) could support biofouling, biofilm formation and material damage through acid production (6,73–75). This is particularly important with respect to fungal growth, as these might affect human health indirectly by causing allergic reactions and asthmatic responses (17,76–78).…”

Section: Discussionmentioning

confidence: 99%

The International Space Station selects for microorganisms adapted to the extreme environment, but does not induce genomic and physiological changes relevant for human health

Mora

Wink

et al. 2019

Preprint

View full text Add to dashboard Cite

The International Space Station (ISS) is a unique, completely confined habitat for the human crew and co-inhabiting microorganisms. Here, we report on the results of the ISS experiment “EXTREMOPHILES”. We aimed to exploit the microbial information obtained from three surface and air sampling events aboard the International Space Station during increments 51 and 52 (2017) with respect to: i) microbial sources, diversity and distribution within the ISS, ii) functional capacity of microbiome and microbial isolates, iii) extremotolerance and antibiotics-resistance (compared to ground controls), and iv) microbial behavior towards ISS-relevant materials such as biofilm formation, or potential for degradation. We used wipe samples and analyzed them by amplicon and metagenomics sequencing, cultivation, comparative physiological studies, antibiotic resistance tests, genome analysis of isolates and co-incubation experiments with ISS-relevant materials. The major findings were: i) the ISS microbiome profile is highly similar to ground-based confined indoor environments, ii) the ISS microbiome is subject to fluctuations and indicative for the (functional) location, although a core microbiome was present over time and independent from location, iii) the ISS selects for microorganisms adapted to the extreme environment, but does not necessarily induce genomic and physiological changes which might be relevant for human health, iv) cleanrooms and cargo seems to be a minor source of microbial contamination aboard, and v) microorganisms can attach to and grow on ISS-relevant materials. Biofilm formation might be a threat for spacecraft materials with the potential to induce instrument malfunctioning with consequences for mission success. We conclude that our data do not raise direct reason for concern with respect to crew health, but indicate a potential threat towards biofilm formation and material integrity in moist areas.

show abstract

Characterization of fungi isolated from the equipment used in the International Space Station or Space Shuttle

Cited by 27 publications

References 26 publications

New endophytic Toxicocladosporium species from cacti in Brazil, and description of Neocladosporium gen. nov.

New endophytic Toxicocladosporium species from cacti in Brazil, and description of Neocladosporium gen. nov.

Space Station conditions are selective but do not alter microbial characteristics relevant to human health

The International Space Station selects for microorganisms adapted to the extreme environment, but does not induce genomic and physiological changes relevant for human health

Contact Info

Product

Resources

About