Characterization of GABA-Transaminase Gene from Mulberry (Morus multicaulis) and Its Role in Salt Stress Tolerance

Zhang, Mengru; Fan, Yiting; Liu, Chaorui; Wang, Hairui; Li, Yan; Xin, Youchao; Gai, Yingping; Ji, Xiaodong

doi:10.3390/genes13030501

Cited by 23 publications

(11 citation statements)

References 57 publications

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“…In this study, salt stress led to an increase in H 2 O 2 content and a concomitant increase in MDA content in the leaves of ‘Tahe 2’ and ‘Xinluzhong 62’ seedlings. This aligned with previous observations of salt-induced oxidative stress in different plants [ 4 , 14 , 20 , 35 , 36 ]. Under salt stress, compared to the no GABA treatment, treatment with 1~4 mM GABA reduced the H 2 O 2 and malondialdehyde (MDA) content in cotton seedlings, consistent with previous research findings [ 14 , 15 , 20 , 35 , 36 ].…”

Section: Discussionsupporting

confidence: 92%

“…Under salt stress, exogenous GABA treatment increased the activities of SOD and POD in cotton seedlings. This alignment with previous research findings [ 14 , 35 ] further indicated that GABA could alleviate oxidative damage caused by salt stress through direct or indirect mechanisms [ 10 , 11 ]. Interestingly, the impact of exogenous GABA on CAT activity in cotton seedlings of ‘Tahe 2’ and ‘Xinluzhong 62’ under salt stress was the opposite.…”

Section: Discussionsupporting

confidence: 92%

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Exogenous γ-Aminobutyric Acid Can Improve Seed Germination and Seedling Growth of Two Cotton Cultivars under Salt Stress

Dong,

Huang,

et al. 2023

Plants

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Excessive salt content in soil has adverse effects on cotton production, especially during the germination and seedling stages. γ-aminobutyric acid (GABA) is an important active substance that is expected to improve the resistance of plants to abiotic stresses. This study focused on two cotton cultivars (Gossypium hirsutum L.: Tahe 2 and Xinluzhong 62) and investigated the impact of exogenous GABA (0, 1, 2, 3, and 4 mM) on seed germination, seedling growth, and related morphological, physiological, and biochemical indicators under salt stress (150 mM NaCl). The results showed that salt stress significantly reduced the germination rate and germination index of cotton seeds (decreased by 20.34% and 32.14% for Tahe 2 and Xinluzhong 62, respectively), leading to decreased seedling height and biomass and causing leaf yellowing. Salt stress induced osmotic stress in seedlings, resulting in ion imbalance (marked reduction in K+/Na+ ratio) and oxidative damage. Under salt stress conditions, exogenous GABA increased the germination rate (increased by 10.64~23.40% and 2.63~31.58% for Tahe 2 and Xinluzhong 62, respectively) and germination index of cotton seeds, as well as plant height and biomass. GABA treatment improved leaf yellowing. Exogenous GABA treatment increased the content of proline and soluble sugars, with varying effects on betaine. Exogenous GABA treatment reduced the Na+ content in seedlings, increased the K+ content, and increased the K+/Na+ ratio (increased by 20.44~28.08% and 29.54~76.33% for Tahe 2 and Xinluzhong 62, respectively). Exogenous GABA treatment enhanced the activities of superoxide dismutase and peroxidase, and reduced the accumulation of hydrogen peroxide and malondialdehyde, but had a negative impact on catalase activity. In conclusion, exogenous GABA effectively improved cotton seed germination. By regulating osmoprotectant levels, maintaining ion homeostasis, and alleviating oxidative stress, GABA mitigated the adverse effects of salt stress on cotton seedling growth.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 92%

Exogenous γ-Aminobutyric Acid Can Improve Seed Germination and Seedling Growth of Two Cotton Cultivars under Salt Stress

Dong,

Huang,

et al. 2023

Plants

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“…These results suggest that the GABA-T mutation may prevent GABA degradation, which would affect plants’ ability to grow normally [ 31 ]. In addition, similar to GAD , GABA-T expression is also induced by a variety of stress conditions [ 23 , 25 , 32 ], GABA-T overexpressed plants have been seen to be more sensitive to salt stress in mulberry [ 33 ]. The increase in GABA in the root and above-ground parts of gaba-t seedlings has been seen to be significantly higher than that of the wild type, indicating that the seedling is more sensitive to salt stress than the wild type.…”

Section: Synthesis and Metabolism Of Gabamentioning

confidence: 99%

Role of Gamma-Aminobutyric Acid in Plant Defense Response

et al. 2023

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Gamma-aminobutyric acid (GABA) is a four-carbon non-protein amino acid that acts as a defense substance and a signaling molecule in various physiological processes, and which helps plants respond to biotic and abiotic stresses. This review focuses on the role of GABA’s synthetic and metabolic pathways in regulating primary plant metabolism, redistributing carbon and nitrogen resources, reducing the accumulation of reactive oxygen species, and improving plants’ tolerance of oxidative stress. This review also highlights the way in which GABA maintains intracellular pH homeostasis by acting as a buffer and activating H+-ATPase. In addition, calcium signals participate in the accumulation process of GABA under stress. Moreover, GABA also transmits calcium signals through receptors to trigger downstream signaling cascades. In conclusion, understanding the role of GABA in this defense response provides a theoretical basis for applying GABA in agriculture and forestry and feasible coping strategies for plants in complex and changeable environments.

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“…Thus, the study revealed that GABA accumulation under drought stress appears to be involved in stomatal regulation [ 252 ]. Exogenous application of GABA in mulberry resulted in increased antioxidant enzyme activity and decreased oxygen-induced injury associated with increased salt resistance [ 253 ]. In addition, GABA appears to act as a signaling molecule in flooding stress by activating H 2 O 2 signaling and preventing cells from entering programmed cell death (PCD) [ 254 ].…”

Section: Role Of Secondary Metabolites Under Abiotic Stressmentioning

confidence: 99%

Ethylene and Jasmonates Signaling Network Mediating Secondary Metabolites under Abiotic Stress

Pérez-Llorca

Pollmann

Müller

2023

IJMS

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Plants are sessile organisms that face environmental threats throughout their life cycle, but increasing global warming poses an even more existential threat. Despite these unfavorable circumstances, plants try to adapt by developing a variety of strategies coordinated by plant hormones, resulting in a stress-specific phenotype. In this context, ethylene and jasmonates (JAs) present a fascinating case of synergism and antagonism. Here, Ethylene Insensitive 3/Ethylene Insensitive-Like Protein1 (EIN3/EIL1) and Jasmonate-Zim Domain (JAZs)-MYC2 of the ethylene and JAs signaling pathways, respectively, appear to act as nodes connecting multiple networks to regulate stress responses, including secondary metabolites. Secondary metabolites are multifunctional organic compounds that play crucial roles in stress acclimation of plants. Plants that exhibit high plasticity in their secondary metabolism, which allows them to generate near-infinite chemical diversity through structural and chemical modifications, are likely to have a selective and adaptive advantage, especially in the face of climate change challenges. In contrast, domestication of crop plants has resulted in change or even loss in diversity of phytochemicals, making them significantly more vulnerable to environmental stresses over time. For this reason, there is a need to advance our understanding of the underlying mechanisms by which plant hormones and secondary metabolites respond to abiotic stress. This knowledge may help to improve the adaptability and resilience of plants to changing climatic conditions without compromising yield and productivity. Our aim in this review was to provide a detailed overview of abiotic stress responses mediated by ethylene and JAs and their impact on secondary metabolites.

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Characterization of GABA-Transaminase Gene from Mulberry (Morus multicaulis) and Its Role in Salt Stress Tolerance

Cited by 23 publications

References 57 publications

Exogenous γ-Aminobutyric Acid Can Improve Seed Germination and Seedling Growth of Two Cotton Cultivars under Salt Stress

Exogenous γ-Aminobutyric Acid Can Improve Seed Germination and Seedling Growth of Two Cotton Cultivars under Salt Stress

Role of Gamma-Aminobutyric Acid in Plant Defense Response

Ethylene and Jasmonates Signaling Network Mediating Secondary Metabolites under Abiotic Stress

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