Characterization of genes expressed in Casuarina equisetifolia in response to elicitation by cell wall components of Trichosporium vesiculosum

Abstract

Casuarina equisetifolia has the widest distribution of all Casuarina species and is a nitrogen-fixing tree planted in tropical/subtropical littoral zones of Asia, the Pacific and Africa for coastal reclamation, charcoal making, pulp and timber. Trichosporium vesiculosum, the causal agent of blister bark disease, is a serious pathogen of C. equisetifolia. The present study was undertaken to understand the molecular mechanisms involved during pathogen cell wall elicitation in this hardy tree species. Transcript profiling during elicitation induced by cell wall components of T. vesiculosum revealed expression of resistance genes; cytochrome oxidase; trans membrane proteins; genes involved in programmed cell death like 26S proteasome and ubiquitin activating enzyme; early nodulin gene, wound inducible metallocarboxy peptidase inhibitor, glucanase, metal binding protein and signal recognition particle. The fold expression of selected transcripts including glucanase, 26 S proteasome, signal recognition particle, cytochrome oxidase and the metal binding protein using RT-qPCR revealed 12-59 fold increase in expression after 48 hours of elicitor treatment. The expression of these transcripts during abiotic stresses like heat, mechanical wounding, salt (NaCl) and drought (PEG) was also analyzed. Glucanase was up-regulated significantly during wounding and heat stress while proteasome was up-regulated 1-4 fold during NaCl, PEG and wounding stress. The novel transcript CeHMA was up-regulated under all the stress conditions. This is the first report on molecular defense in C. equisetifolia and has provided a pool of candidate genes for detailed molecular dissection to further broaden the knowledge on the response of woody perennials during pathogen cell wall elicitation.

Genomics studies for trait improvement in four important tree species: Current status and future prospects

“…Other functional genomics achievements include identifying cellulose, hemicellulose, and lignin-related genes related to secondary growth, chlorophyll synthesis, cell wall biosynthesis, and secondary cell wall synthesis in different Casuarina species reported by Ye et al (2019). The molecular defence in Casuarina equisetifolia by the novel transcript CeHMA, which was upregulated under biotic and abiotic stress conditions, broadened the knowledge of the response of woody perennials during pathogen cell wall elicitation (Modhumita and Karpaga, 2017). These genomics studies can enhance genomic resources to elucidate the functional understanding of Casuarina trees further and will be useful in genetic improvement.…”