Characterization of Genetic Basis on Synergistic Interactions between Root Architecture and Biological Nitrogen Fixation in Soybean

Yang, Yongqing; Zhao, Qingsong; Li, Xinxin; Ai, Wenqin; Liu, Dong; Qi, Wandong; Zhang, Mengchen; Yang, Chen; Liao, Hong

doi:10.3389/fpls.2017.01466

Cited by 45 publications

(59 citation statements)

References 75 publications

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“…In 2 years of field trials, both CPCoA and ANOSIM results suggest that soybean genotypes significantly influence the structure and composition of bacterial communities (Figure 2). Although we have not yet identified the genes responsible for the control of nodulation in soybean, nodulation traits are indeed determined by the genetic traits of Yang, Zhao, et al (2017), who found that three BNF QTLs could explain over 30% of the variation in soybean nodulation traits. Interestingly, the three high nodulation RILs studied herein carried at least two of the three BNF QTLs, whereas the three low nodulation RILs did not carry any of the three BNF QTLs ( Figure S1).…”

Section: Rhizobium Inoculation Changes the Co-occurrence Network Ofmentioning

confidence: 93%

“…Two soybean cultivars (P1: low nodulation and P2: high nodulation) contrasting in nodulation and a previously identified set of six RILs with high‐nodulation (HN1–3) and low‐nodulation (LN1–3) phenotypes (Yang et al, ) were included in this study. The rhizobia used for inoculation were previously isolated from soybean nodules (Chen et al, ).…”

Section: Methodsmentioning

confidence: 99%

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Genotype and rhizobium inoculation modulate the assembly of soybean rhizobacterial communities

Zhong

Yang

Liu

et al. 2019

Plant Cell & Environment

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Rhizosphere bacterial communities are vital for plants, yet the composition of rhizobacterial communities and the complex interactions between roots and microbiota, or between microbiota, are largely unknown. In this study, we investigated the structure and composition of rhizobacterial communities in two soybean cultivars and their recombinant inbred lines contrasting in nodulation through 16S rRNA amplicon sequencing in two years of field trials. Our results demonstrate that soybean plants are able to select microbes from bulk soils at the taxonomic and functional level. Soybean genotype significantly influenced the structure of rhizobacterial communities and resulted in dramatically different co‐occurrence networks of rhizobacterial communities between different genotypes of soybean plants. Furthermore, the introduction of exogenous rhizobia through inoculation altered soybean rhizobacterial communities in genotype‐dependent manner. Rhizobium inoculation not only stimulated the proliferation of potential beneficial microbes but also increased connections in rhizobacterial networks and changed the hub microbes, all of which led to the association of distinctive bacterial communities. Taken together, we demonstrated that the assembly of soybean rhizobacterial communities was determined by both genotype and the introduction of exogenous rhizobia. These findings bolster the feasibility of root microbiome engineering through inoculation of specific microbial constituents.

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Section: Rhizobium Inoculation Changes the Co-occurrence Network Ofmentioning

confidence: 93%

Section: Methodsmentioning

confidence: 99%

Genotype and rhizobium inoculation modulate the assembly of soybean rhizobacterial communities

Zhong

Yang

Liu

et al. 2019

Plant Cell & Environment

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“…Two soybean cultivars contrasting in nodulation (P1, Low nodulation; P2, high nodulation), together with eight of their F 9:11 recombinant inbred lines (RILs; HN1-4, four lines with high nodulation; LN1-4, four lines with low nodulation) were tested. The RIL lines were selected based on nodule number and the presence (HN) or absence (LN) of at least two QTLs responsible for biological nitrogen fixation (BNF) (Supplementary Figure S1 and Supplementary Table S8; Yang et al, 2017). Field trials were conducted from June to October in 2016 and 2017 on the experimental farm (E114.48 • , N38.03 • ) of the Institute of Cereal and Oil crops, Shijiazhuang City, Heibei Province, China.…”

Section: Plant Materials and Field Trialsmentioning

confidence: 99%

“…Besides, the Bradyrhizobium spp. strains have been used to identified the QTLs responsible for BNF (Yang et al, 2017).…”

Section: Plant Materials and Field Trialsmentioning

confidence: 99%

“…To date, the extent to which rhizobium introduction influences fungal communities is unclear, with few interactions between rhizobium and fungal community members of soybean rhizospheres revealed. As hosts, this study included two parental soybean cultivars contrasting in nodulation (P1: low nodulation, P2: high nodulation), along with their low nodulation (LN1-4) and high nodulation (HN1-4) recombinant inbred lines (RILs) that also varied across markers for BNF (Biological Nitrogen Fixation), as determined by q BNF QTL (Yang et al, 2017). ITS1 region amplicon sequencing was used to investigate the structure of fungal communities in bulk soils and in rhizospheres of the two parental soybean lines and their RILs grown under rhizobium inoculation and non-inoculation conditions, in order to reveal the potential complex interactions among rhizobia, soybean plants and fungal community.…”

Section: Introductionmentioning

confidence: 99%

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Rhizobium Inoculation Drives the Shifting of Rhizosphere Fungal Community in a Host Genotype Dependent Manner

Yang

Tian

et al. 2020

Front. Microbiol.

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Rhizosphere microorganisms play important roles in plant health and nutrition, and interactions among plants and microorganisms are important for establishment of root microbiomes. As yet, plant-microbe and microbe-microbe interactions in the rhizosphere remain largely mysterious. In this study, rhizosphere fungal community structure was first studied in a field experiment with two soybean cultivars contrasting in nodulation grown in two rhizobium inoculation treatments. Following this, recombinant inbred lines (RILs) contrasting in markers across three QTLs for biological nitrogen fixation (BNF) were evaluated for effects of genotype and rhizobium inoculation to the rhizosphere fungal community as assessed using ITS1 amplicon sequencing. The soybean plants tested herein not only hosted rhizosphere fungal communities that were distinct from bulk soils, but also specifically recruited and enriched Cladosporium from bulk soils. The resulting rhizosphere fungal communities varied among soybean genotypes, as well as, between rhizobium inoculation treatments. Besides, Cladosporium were mostly enriched in the rhizospheres of soybean genotypes carrying two or three favorable BNF QTLs, suggesting a close association between soybean traits associated with nodulation and those affecting the rhizosphere fungal community. This inference was bolstered by the observation that introduction of exogenous rhizobia significantly altered rhizosphere fungal communities to the point that these communities could be distinguished based on the combination of soybean genotype and whether exogenous rhizobia was applied. Interestingly, grouping of host plants by BNF QTLs also distinguished fungal community responses to rhizobium inoculation. Taken together, these results reveal that complex crosskingdom interactions exist among host plants, symbiotic N 2 fixing bacteria and fungal communities in the soybean rhizosphere.

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Soybean growth and production as affected by struvite as a phosphorus source in eastern Arkansas

et al. 2022

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Struvite (MgNH 4 PO 4 •6H 2 O) has been precipitated from liquid waste streams to recover valuable nutrients, such as phosphorus (P) and nitrogen (N), that can be used as an alternative fertilizer-P source. Because prior research has focused on greenhouse studies, it is necessary to expand struvite evaluations to the field-scale to include row-crop responses. The objective of this field study was to evaluate the effects of two struvite materials (electrochemically precipitated struvite, ECST; and chemically precipitated struvite, CPST) relative to other common fertilizer-P sources (diammonium phosphate, DAP; triple superphosphate, TSP; rock phosphate, RP; and monoammonium phosphate, MAP) on soybean [Glycine max (L.) Merr.] response and economics in two consecutive growing seasons in a P-deficient, silt-loam soil (Aquic Fraglossudalfs) in eastern Arkansas. Averaged across years, soybean aboveground tissue P uptake was largest (P < .05) from ECST (28.4 kg ha −1 ), which was similar to CPST (26.7 kg ha −1 ) and TSP (25.9 kg ha −1 ) and was smallest from RP (21.4 kg ha −1 ). In 2019, seed yield was largest (P < .05) from ECST (4.1 Mg ha −1 ), which was similar to DAP, CPST, RP, TSP, and MAP, and was smallest from the unamended control (3.6 Mg ha −1 ). In 2020, seed yield was numerically greatest from CPST (2.8 Mg ha −1 ) and was numerically smallest from ECST (2.2 Mg ha −1 ). Results showed that wastewater-recovered struvite materials have the potential to be a viable, alternative fertilizer-P source for soybean production in a P-deficient, silt-loam soil, but further work is needed to confirm struvite's cost effectiveness.

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Characterization of Genetic Basis on Synergistic Interactions between Root Architecture and Biological Nitrogen Fixation in Soybean

Cited by 45 publications

References 75 publications

Genotype and rhizobium inoculation modulate the assembly of soybean rhizobacterial communities

Genotype and rhizobium inoculation modulate the assembly of soybean rhizobacterial communities

Rhizobium Inoculation Drives the Shifting of Rhizosphere Fungal Community in a Host Genotype Dependent Manner

Soybean growth and production as affected by struvite as a phosphorus source in eastern Arkansas

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