Characterization of genetic variance within and among five populations of Sperata seenghala (Skyes, 1839) revealed by random amplified polymorphic DNA markers

Garg, R. K.; Sairkar, Pramod; Chouhan, Shweta; Batav, Nitin; Silawat, N.; Sharma, Rama; Singh, R. K.; Mehrotra, Nitin

doi:10.1016/j.jgeb.2013.11.001

Cited by 6 publications

(5 citation statements)

References 9 publications

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“…These results indicated high genetic polymorphism using RAPD markers, which means that allele frequencies and molecular polymorphism can be examined using RAPD markers. The RAPD approach is reliable and uncomplicated, and manageable [2].…”

Section: Phylogenetic Analysismentioning

confidence: 99%

“…Because of numerous environmental factors, biodiversity documentation has grown in importance as a scientific endeavor in recent years [1]. One of the largest issues facing contemporary society is the loss of biodiversity, as the depletion of genetic resources and habitats is a growing indicator of environmental disaster [2]. A species genetic variability and stock structure must be known in advance for scientific management, genetic improvement programs to prevent population decline in the species native habitat and insure the sustainability of its resources [3].…”

Section: Introductionmentioning

confidence: 99%

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Pattern and Dynamics of Genetic Stock of Freshwater Fishes Using Various Molecular Markers for Their Conservation Management Concerns in the Indian Riverine System

Batham,

Garg

2024

Asian J. Biochem. Gen. Mol. Biol.

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India has a world's richest, most abundant and most promising inland fisheries resources with variety fishes in tributaries, streams, canals, lakes, ponds, and reservoirs. There are several significant river systems in India, including the Cauvery, Tapi, Narmada, Krishna, Indus, Brahmaputra, Ganga, Mahanadi, and Godavari which showing shrinkage of fish fauna because of environmental disturbance, human interference and human threats. In order to conserve the fish diversity, the molecular markers are helpful in determining genetic diversity, gene polymorphism and gene flows from generation to generation. The topic experienced a surge in interest due to the introduction of strong statistical analysis tools and the accessibility of DNA fingerprinting, DNA sequencing techniques and consequently population genetic studies. Some molecular markers, Microsatellite markers, RAPD, Allozymes and mitochondrial (cox1, cytob, ATPase6/8) implications have been discussed in this review which will provide an overview that have been used by scientists to studied population genetic structure and genetic variations at various levels.

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Section: Phylogenetic Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Pattern and Dynamics of Genetic Stock of Freshwater Fishes Using Various Molecular Markers for Their Conservation Management Concerns in the Indian Riverine System

Batham,

Garg

2024

Asian J. Biochem. Gen. Mol. Biol.

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“…"Challenges of tomatoes due to changes associated to climate conditions, use of pesticides against agricultural pests, inadequate rainfall and microorganisms particularly fungi can make tomatoes unsafe to human consumption. One of the limiting factors that influence tomato economic value is its relatively short shelf life caused by pathogen attack" [1,2,3].…”

Section: Introductionmentioning

confidence: 99%

“…"The microorganisms found in waste dump sites obtain their nutritional requirements from the waste constituents, thus aiding the detoxification of complex organic molecules" [2].…”

Section: Introductionmentioning

confidence: 99%

Major Component for the Isolation and Identification of Microorganisms Found in Tomato Soil at Akwata Garriki

Ngozi,

Jacinta,

Ifeanyi

2023

AJEE

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This study was conducted to isolate and identify microorganisms from swamp water samples from the Akwata market in Enugu, where tomatoes were sold. Three wetland water samples, one each at the entrance, middle and end of the Akwata market were collected and analyzed for the presence of bacteria and fungi. The microorganisms were isolated by culturing on Nutrient, MacConkey and Sabouraud glucose agar plates using standard methods. After the incubation period, the number of unit-forming colonies was counted to determine the average total bacterial count. Bacterial isolates were identified based on colony, microscopic and biochemical reactions. Fungal isolates were identified based on their morphological and microscopic features. Wetland water samples taken at the entrance, middle, and edge of the Akwata market had an average total bacterial count of 1.4 x 104 CFU/mL, 2.0 x 104 CFU/mL, and 1.5 x 104 CFU/mL. Fungal counts were 1.6 x 104 CFU/mL, 3.5 x 104 CFU/mL, and 2.4 x 104 CFU/mL, respectively, in swamp water samples collected at the entrance collected at the middle and end of the Akwata market. Bacterial isolates identified were Escherichia coli (27.58%), Pseudomonas sp. (7.93%), Klebsiella spp. (20.7%) and Salmonella spp. (13.79%). The identified fungal isolate was Aspergillus sp. (38.88%), Candida sp. (16.67%), Fusarium sp. (27.78) and Penicillium sp. (16.67%). This study showed that bacteria and fungi are associated with the swamp water of the Akwata market where tomatoes were sold. Efforts should therefore be made to improve the quality of the water supplied to the Akwata market and thus guarantee the hygienic and sanitary quality of the tomatoes sold.

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“…S. seenghala and S. aor are recognized as “least concern” in the RedList of International Union for Conservation of Nature (IUCN). Previous studies on giant river catfish gradually focused on the genetic assessment of S. seenghala using Randomly amplified polymorphic DNA (RAPD) marker (Saini et al , 2010; Garg et al , 2014), mtDNA markers (Kumari et al , 2017), and microsatellite marker (Acharya et al , 2019). The microsatellite-based stock discrimination of S. aor revealed the existence of three genetic stocks from the connected vicinity of the Ganga river (Nazir & Khan, 2017).…”

Section: Introductionmentioning

confidence: 99%

Molecular evidence indicates the existence of multiple lineages ofSperata speciesin Indian Rivers

Yadav

Kumar

Yadav

et al. 2020

Preprint

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Sperata seenghala (Giant river-catfish) and Sperata aor (Long-whiskered catfish) are commercially important freshwater catfishes of India, belongs to family Bagridae. Due to high nutritional significance and the low number of intramuscular bones, both fishes have considerable demand in South Asian countries. Both of the Sperata species are morphologically close and well adapted to the same habitat. In this study, we have assessed the level of genetic diversity and differentiation of S. seenghala and S. aor in the Ganga River based on the mitochondrial DNA (mtDNA) control region and compared with the other major Indian rivers. We found high haplotypes diversity for both the species in the Ganga. However, it was comparatively low for S. seenghala in Mahanadi and Brahmaputra populations. The phylogenetic and median-joining network strongly indicated the presence of two distinct maternal lineages of S. seenghala from the Ganga river. Interestingly, the genetic differentiation between S. seenghala of Ganga-Brahmaputra was much higher (~25.3%) than the S. seenghala and S. aor (~17%), whereas it was comparatively low between Ganges-Mahanadi (~8.0%). Our finding provided evidence that all the three rivers: Ganga, Mahanadi, and the Brahmaputra sustain a highly diverse and genetically distinct stock of giant river catfish; therefore, all populations should be considered as a different management unit for the protection of stocks. Our findings indicated that Brahmaputra lineages qualify the species level variations. This study can be further used as a reference database for proper lineage identification of S. seenghala and S. aor that could formulate the appropriate conservation and management plans.

show abstract

Characterization of genetic variance within and among five populations of Sperata seenghala (Skyes, 1839) revealed by random amplified polymorphic DNA markers

Cited by 6 publications

References 9 publications

Pattern and Dynamics of Genetic Stock of Freshwater Fishes Using Various Molecular Markers for Their Conservation Management Concerns in the Indian Riverine System

Pattern and Dynamics of Genetic Stock of Freshwater Fishes Using Various Molecular Markers for Their Conservation Management Concerns in the Indian Riverine System

Major Component for the Isolation and Identification of Microorganisms Found in Tomato Soil at Akwata Garriki

Molecular evidence indicates the existence of multiple lineages ofSperata speciesin Indian Rivers

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