Characterization of Gibberellin Biosynthetic Gene Cluster from Fusarium proliferatum

Rim, Soon Ok; You, Young Hyun; Yoon, Hyeok Jun; Kim, Ye Eun; Lee, Jin Hyung; Kang, Myung Suk; Kim, Chang Mu; Seu, Young‐Bae; Kim, Jong Guk

doi:10.4014/jmb.1212.12029

Cited by 9 publications

(5 citation statements)

References 28 publications

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“…The presence of aforementioned genes involved in the GAs biosynthesis pathway validated the GA producing capability of endophytic P. formosus LHL10. Previously, same GA gene cluster has also been reported in Fusarium fujikuroi , Gibberella fujikuroi , and Fusarium proliferatum (Tudzynski et al, 2001; Albermann et al, 2013; Rim et al, 2013). However, there is no information on GA gene biosynthetic pathway in Paecilomyces sp.…”

Section: Discussionsupporting

confidence: 69%

Endophytic Paecilomyces formosus LHL10 Augments Glycine max L. Adaptation to Ni-Contamination through Affecting Endogenous Phytohormones and Oxidative Stress

et al. 2017

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This study investigated the Ni-removal efficiency of phytohormone-producing endophytic fungi Penicillium janthinellum, Paecilomyces formosus, Exophiala sp., and Preussia sp. Among four different endophytes, P. formosus LHL10 was able to tolerate up to 1 mM Ni in contaminated media as compared to copper and cadmium. P. formosus LHL10 was further assessed for its potential to enhance the phytoremediation of Glycine max (soybean) in response to dose-dependent increases in soil Ni (0.5, 1.0, and 5.0 mM). Inoculation with P. formosus LHL10 significantly increased plant biomass and growth attributes as compared to non-inoculated control plants with or without Ni contamination. LHL10 enhanced the translocation of Ni from the root to the shoot as compared to the control. In addition, P. formosus LHL10 modulated the physio-chemical apparatus of soybean plants during Ni-contamination by reducing lipid peroxidation and the accumulation of linolenic acid, glutathione, peroxidase, polyphenol oxidase, catalase, and superoxide dismutase. Stress-responsive phytohormones such as abscisic acid and jasmonic acid were significantly down-regulated in fungal-inoculated soybean plants under Ni stress. LHL10 Ni-remediation potential can be attributed to its phytohormonal synthesis related genetic makeup. RT-PCR analysis showed the expression of indole-3-acetamide hydrolase, aldehyde dehydrogenase for indole-acetic acid and geranylgeranyl-diphosphate synthase, ent-kaurene oxidase (P450-4), C13-oxidase (P450-3) for gibberellins synthesis. In conclusion, the inoculation of P. formosus can significantly improve plant growth in Ni-polluted soils, and assist in improving the phytoremediation abilities of economically important crops.

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Section: Discussionsupporting

confidence: 69%

Endophytic Paecilomyces formosus LHL10 Augments Glycine max L. Adaptation to Ni-Contamination through Affecting Endogenous Phytohormones and Oxidative Stress

et al. 2017

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“…The experiment was repeated 3 times. (Bömke et al 2008;Bömke and Tudzynski 2009;Rim et al 2013). However, the current findings of GA biosynthesis are to some extent similar to the Sphaceloma spp.…”

Section: Discussionsupporting

confidence: 67%

Preussia sp. BSL-10 producing nitric oxide, gibberellins, and indole acetic acid and improving rice plant growth

Al-Hosni

Shahzad

Khan

et al. 2018

Journal of Plant Interactions

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Preussia sp. have been least known to improve plant growth and produce phytohormones. The current study investigated the production of nitric oxide (NO), indole-3-acetic acid (IAA), and gibberellins (GA 4 , GA 7 , GA 15 , and GA 53) by a novel endophytic-fungal strain Preussia sp. BSL-10 using advanced chromatographic and spectroscopic techniques. Production of these phytohormones were validated by RT-PCR analysis, which indicated the expression of genes encoding tryptophan synthase (TRP), indole-3-acetamide hydrolase (IAAH), tryptophan-2-monooxygenase (IAAM), aldehyde dehydrogenase (ALD), GA 4 desaturase (DES), geranylgeranyl-diphosphate synthase (GGS2), entdesaturase oxidase (P450-4), GA 14 synthase (P450-1) and nitrite reductase (NIRK/NIRS), cytochrome P450 (P450nor), nitrate reductase (NR), NOS-like (NOL), and nitric oxide reductase (QNOR/CNOR). In plant growth-promoting effects, the inoculation of Preussia sp. BSL-10 significantly increased the growth of dwarf mutant Waito-C and wild-type rice cultivars. In conclusion, utilizing new endophytic with the ability to produce NO, IAA, and gibberellins can be used to promote growth and yield of marginalized crops.

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“…Copalyl diphosphate is an intermediate component for biosynthesis of ent -kaurene which, in turn, is an intermediate component of gibberellin biosynthesis ( Hedden & Kamiya, 1997 ). Gibberellin biosynthesis pathway requires additional gene of gibberellic acid (GA 4 ) desaturase ( des ) and four P450 classes of oxidases (GA 14 synthase (P450-1), GA 20 oxidase (P450-2), and C 13 -oxidase (P450-3)) ( Rim et al, 2013 ) aside from genes found in an ent -kaurene gene cluster. des , P450-1, P450-2, and P450-3 genes are not specifically annotated in UM 591 genome data, but there is one P450 gene (UM591_10049) within the putative ent -kaurene cluster.…”

Section: Resultsmentioning

confidence: 99%

Genomic insight into pathogenicity of dematiaceous fungusCorynespora cassiicola

Looi

Toh

Yew

et al. 2017

PeerJ

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Corynespora cassiicola is a common plant pathogen that causes leaf spot disease in a broad range of crop, and it heavily affect rubber trees in Malaysia (Hsueh, 2011; Nghia et al., 2008). The isolation of UM 591 from a patient’s contact lens indicates the pathogenic potential of this dematiaceous fungus in human. However, the underlying factors that contribute to the opportunistic cross-infection have not been fully studied. We employed genome sequencing and gene homology annotations in attempt to identify these factors in UM 591 using data obtained from publicly available bioinformatics databases. The assembly size of UM 591 genome is 41.8 Mbp, and a total of 13,531 (≥99 bp) genes have been predicted. UM 591 is enriched with genes that encode for glycoside hydrolases, carbohydrate esterases, auxiliary activity enzymes and cell wall degrading enzymes. Virulent genes comprising of CAZymes, peptidases, and hypervirulence-associated cutinases were found to be present in the fungal genome. Comparative analysis result shows that UM 591 possesses higher number of carbohydrate esterases family 10 (CE10) CAZymes compared to other species of fungi in this study, and these enzymes hydrolyses wide range of carbohydrate and non-carbohydrate substrates. Putative melanin, siderophore, ent-kaurene, and lycopene biosynthesis gene clusters are predicted, and these gene clusters denote that UM 591 are capable of protecting itself from the UV and chemical stresses, allowing it to adapt to different environment. Putative sterigmatocystin, HC-toxin, cercosporin, and gliotoxin biosynthesis gene cluster are predicted. This finding have highlighted the necrotrophic and invasive nature of UM 591.

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Characterization of Gibberellin Biosynthetic Gene Cluster from Fusarium proliferatum

Cited by 9 publications

References 28 publications

Endophytic Paecilomyces formosus LHL10 Augments Glycine max L. Adaptation to Ni-Contamination through Affecting Endogenous Phytohormones and Oxidative Stress

Endophytic Paecilomyces formosus LHL10 Augments Glycine max L. Adaptation to Ni-Contamination through Affecting Endogenous Phytohormones and Oxidative Stress

Preussia sp. BSL-10 producing nitric oxide, gibberellins, and indole acetic acid and improving rice plant growth

Genomic insight into pathogenicity of dematiaceous fungusCorynespora cassiicola

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